Cell-Penetrating Peptides as Carriers for Transepithelial Drug Delivery

Stereochemistry and Intermolecular Interactions Influence Carrier Peptide-Mediated Insulin Delivery

“…Following TEER measurements in the insulin permeation study, the Caco-2 cell monolayers in filter inserts were kept with 350 μL MTS-PMS solution (240 μg/mL MTS and 2.4 μg/mL PMS in HEPES-HBSS) and 1000 μL RT HEPES-HBSS on the apical and basolateral sides, respectively, on the shaking table (50 rpm, 37 °C) for 1.5 h. A volume of 100 μL sample was transferred from the apical side to clear 96-well plates, and the absorbance was measured at 492 nm and subtracted the background at 630 nm using the FLUOstar Omega plate reader (BMG Labtech). The Caco-2 cell monolayers were washed twice in 37 °C HEPES-HBSS and restituted in the growth medium in the incubator (5% CO 2 , 37 °C) for 1 d prior to repeated TEER measurements and MTS-PMS assay as previously described …”

“…The Caco-2 cell monolayers were washed twice in 37 °C HEPES-HBSS and restituted in the growth medium in the incubator (5% CO 2 , 37 °C) for 1 d prior to repeated TEER measurements and MTS-PMS assay as previously described. 14 Real-time cellular viability of proliferating Caco-2 cells incubated with 100 μL test sample was evaluated using a RealTime-Glo MT cell viability assay kit (Promega) as described by the manufacturer. The Caco-2 cells were incubated in a CLARIOstar Plus plate reader (BMG Labtech; 37 °C) for 3 h with measurements of the luminescence at 420−510 nm every 2 min.…”