Cell penetrable, clickable and tagless activity-based probe of human cathepsin L

Zebrafish as a Translational Model: An Experimental Alternative to Study the Mechanisms Involved in Anosmia and Possible Neurodegenerative Aspects of COVID-19?

“…These enzymes could be used as a target for new drug development. A methodology to evaluate cathepsin L activity was already developed and tested in zebrafish ( Dana et al, 2019 ). It was developed for different purposes, happened before SARS-CoV-2 was brought to light, but it could be helpful to assess the efficacy of drugs that target cathepsin L.…”

“…Cathepsin L is involved in the development and hatching processes (Vogel and Gerster, 1997). Dana and colleagues (2019) used zebrafish as the in vivo model to ensure the efficacy of a selective probe synthesized to study human cathepsin L (Dana et al, 2019). Therefore, cathepsin L was proposed as a biomarker for chemical exposure by evaluating its enzymatic activity in zebrafish embryos (Küster, 2005).…”

Coumarin as a structural component of substrates and probes for serine and cysteine proteases

“…Three-component probes have been reported for cathepsin B and L that produce in the cleavage step a two-component leaving group which undergoes spontaneous immolation to generate the fluorescent reporter AMC [98,99].…”

The Impact of Chemical Biology on Drug Discovery

“…Fluorescence, despite its limited spatial resolution, can prove to be a valuable tool for evaluation of compound effects not only in cells but also in small rodents. As attachment of bulky and frequently charged fluorophores and quenchers limits the permeability of the obtained probes, simplified clickable probes can contribute to overcome these issues, however their application requires a two‐step protocol with subsequent functionalization.…”