Cell patterning without chemical surface modification: Cell–cell interactions between printed bovine aortic endothelial cells (BAEC) on a homogeneous cell-adherent hydrogel

Chen, Christopher Y.; Barron, Jason A.; Ringeisen, Bradley R.

doi:10.1016/j.apsusc.2005.11.088

Cited by 65 publications

(53 citation statements)

References 27 publications

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“…1 Previously, pulsed laser printing techniques have shown great promise for printing numerous types of mammalian cells. [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] Pulsed laser cell depositions have shown high cell viability, 4,5,8,[11][12][13][14][15][16] little to no DNA damage, 6,16 unaltered apoptosis rates, 8,16 little to no increase in heat shock protein expression, 11,15 and normal cell proliferation. 8,11,16 Current laser-based direct-write techniques have proven their efficacy and potential; however, many rely on commercially available basement membrane matrix, MatrigelÔ (BD Biosciences, Bedford, MA).…”

Section: Introductionmentioning

confidence: 99%

Gelatin-Based Laser Direct-Write Technique for the Precise Spatial Patterning of Cells

Schiele

Chrisey

Corr

2011

Tissue Engineering Part C: Methods

107

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Section: Introductionmentioning

confidence: 99%

Gelatin-Based Laser Direct-Write Technique for the Precise Spatial Patterning of Cells

Schiele

Chrisey

Corr

2011

Tissue Engineering Part C: Methods

107

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“…32 In contrast, the method presented here allows rapid prototyping of cellular patterns depending only on the generated Microsoft Windows Bitmap file and enables a practically infinitive variety of patterning materials as they can be derived from different kinds of monomers and the combinations of them. Other attempts, apart from photolithography, include inkjet-and aerosol-based direct deposition of animal cells, 20,22,50,51 laser-guided direct writing (Odde and Renn, 2000) 51 and the direct spraying of polymers. 32 Inkjetand aerosol-based cell deposition require encapsulation of living cells in droplets.…”

Section: Comparisons With Other Cellular Patterning Methodsmentioning

confidence: 99%

In Situ Nanoliter-Scale Polymer Fabrication for Flexible Cell Patterning

Liberski

Zhang

Bradley

2009

JALA: Journal of the Association for Laboratory Automation

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Drug-testing technologies, biosensor fabrication, tissue engineering, and basic biological research depend strongly on the patterning of live animal cells. Current techniques for controlling cellular adhesion are restricted with two primary limitations. Firstly, the complexity of the available patterns is very limited and, secondly, the pallet of materials that induce cellular patterning is exhaustible. Here, we demonstrate a method for computer-aided control of cell patterning using a scientific inkjet printer that yields a highly complex cellular pattern suitable for applications in regenerative medicine and rapid prototyping, and a strategy for using in situ polymerization for fabrication polymeric patterns directly on-chip.

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“…[67][68][69] The repetition rate of the laser (5 kHz) has showed high cell density (10 8 cells/mL), high-speed (200 mm/s), and highresolution (10 lm) patterning with multiple cell seeding on the scaffolds. 21 Gaebel et al compared the benefit of LIFT-based versus non-LIFT-based tissue engineered cardiac patches for the potential treatment of myocardial infarction.…”

Section: Biomaterials Printing Using Laser Induced Forward Transfermentioning

confidence: 99%

Laser-assisted biofabrication in tissue engineering and regenerative medicine

et al. 2016

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Controlling the spatial arrangement of biomaterials and living cells provides the foundation for fabricating complex biological systems. Such level of spatial resolution (less than 10 lm) is difficult to be obtained through conventional cell processing techniques, which lack the precision, reproducibility, automation, and speed required for the rapid fabrication of engineered tissue constructs. Recently, laserassisted biofabrication techniques are being intensively developed with the use of computer-aided processes for patterning and assembling both living and nonliving materials with prescribed 2D or 3D organization. In this review, we discuss laser-assisted fabrication methods, including laser tweezers, multi-photon polymerization, laser-induced forward transfer (LIFT), matrix assisted pulsed laser evaporation (MAPLE), and laser ablation as well as their applications in biological science and biomedical engineering. These advanced technologies enable the precise manipulation of in vitro cellular microenvironments and the ability to engineer functional tissue constructs with high complexity and heterogeneity, which serve in regenerative medicine, pharmacology, and basic cell biology studies.

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Cell patterning without chemical surface modification: Cell–cell interactions between printed bovine aortic endothelial cells (BAEC) on a homogeneous cell-adherent hydrogel

Cited by 65 publications

References 27 publications

Gelatin-Based Laser Direct-Write Technique for the Precise Spatial Patterning of Cells

Gelatin-Based Laser Direct-Write Technique for the Precise Spatial Patterning of Cells

In Situ Nanoliter-Scale Polymer Fabrication for Flexible Cell Patterning

Laser-assisted biofabrication in tissue engineering and regenerative medicine

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