Cell Patterning Method on a Clinically Ubiquitous Culture Dish Using Acoustic Pressure Generated From Resonance Vibration of a Disk-Shaped Ultrasonic Transducer

Imashiro, Chikahiro; Kurashina, Yuta; Kuribara, Taiki; Hirano, Masao; Totani, Kiichiro; Takemura, Kenjiro

doi:10.1109/tbme.2018.2835834

Cited by 17 publications

(13 citation statements)

References 45 publications

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“…Every proposed new structure oriented to the operation target has to do all the work from beginning to end. [69,70], Lu et al [71], and Imashiro et al [72,73]. (a) Very small micropillers are machined equidistantly along two symmetrical spiral lines.…”

Section: Vibrated Geometric Substratesmentioning

confidence: 99%

“…This effect can be observed in the vibration of the drumhead. Imashiro et al [72,73] proposed a manipulator where a vibrating glass Using silicon substrate vibration to control particle motion is another method. The vibration generated or propagated through the substrate can induce the ARF.…”

Section: Vibrated Geometric Substratesmentioning

confidence: 99%

“…This effect can be observed in the vibration of the drumhead. Imashiro et al [72,73] proposed a manipulator where a vibrating glass substrate was used. Cells amass along with the node of resonance vibration when the vibration is excited on the substrate, since with the resonance vibration, the antinode position largely vibrates while the node position does not.…”

Section: Vibrated Geometric Substratesmentioning

confidence: 99%

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Advances in Micromanipulation Actuated by Vibration-Induced Acoustic Waves and Streaming Flow

et al. 2020

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The use of vibration and acoustic characteristics for micromanipulation has been prevalent in recent years. Due to high biocompatibility, non-contact operation, and relatively low cost, the micromanipulation actuated by the vibration-induced acoustic wave and streaming flow has been widely applied in the sorting, translating, rotating, and trapping of targets at the submicron and micron scales, especially particles and single cells. In this review, to facilitate subsequent research, we summarize the fundamental theories of manipulation driven by vibration-induced acoustic waves and streaming flow. These methods are divided into two types: actuated by the acoustic wave, and actuated by the steaming flow induced by vibrating geometric structures. Recently proposed representative vibroacoustic-driven micromanipulation methods are introduced and compared, and their advantages and disadvantages are summarized. Finally, prospects are presented based on our review of the recent advances and developing trends.

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Section: Vibrated Geometric Substratesmentioning

confidence: 99%

Section: Vibrated Geometric Substratesmentioning

confidence: 99%

Section: Vibrated Geometric Substratesmentioning

confidence: 99%

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Advances in Micromanipulation Actuated by Vibration-Induced Acoustic Waves and Streaming Flow

et al. 2020

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“…However, manual steps present a risk of contamination and result in variable culture efficiency and stability between trials and depending on the technician [6]. In the interest of standardizing these processes, several techniques have been developed, such as cell patterning, detaching, collecting, and harvesting [7]- [12]. Cell counting technologies, which are key to realizing stable and effective cell culture, have been developed, but they require dedicated devices or labor-intensive processes [13], [14].…”

Section: Introductionmentioning

confidence: 99%

Direct Cell Counting Using Macro-Scale Smartphone Images of Cell Aggregates

et al. 2020

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The field of bioengineering depends on technologies for stable cell culture. Conventionally, every process involved in cell culture has been performed manually, so the culture efficiency and stability can vary between trials or depending on the technician. Among these processes, cell counting is particularly important because cell density affects cell function. Conventional cell counting techniques for cell number estimation are inefficient and unstable because they involve the manual work of collecting a sample of the cell suspension. Thus, a cell counting method that is not susceptible to human error is needed. In this study, we present a novel cell counting method based on smartphone imaging and convolutional neural network-based image processing. Cells are aggregated by centrifuging in a tube and then imaged using a smartphone. The image is transferred to a server, and the cell number is predicted using convolutional neural networks on the server. All processes are performed by a custom-developed smartphone-compatible web app. Compared with the conventional method using a hemocytometer, our method yields more stable cell counting. Furthermore, the time and labor required for cell counting are significantly reduced. Our new method could potentially replace conventional cell counting techniques and thus enhance the stability and efficiency of bioengineering studies that require cell culture.

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“…Fluid flow through a microfluidic device applies shear stress to cells cultured in the device [14]. Vibrational stimulation is gaining attention because it is easy to apply in the ubiquitous cell culture dish format and is highly biocompatibile [15]. The method using vibration stimulation has the advantage that the culture environment is not changed, compared with the conventional migration regulation method represented by using chemical stimulation.…”

mentioning

confidence: 99%

Collective cell migration of fibroblasts is affected by horizontal vibration of the cell culture dish

Enomoto

Imashiro

Takemura

2020

Engineering in Life Sciences

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Regulating the collective migration of cells is an important issue in bioengineering. Enhancing or suppressing cell migration and controlling the migration direction is useful for various physiological phenomena such as wound healing. Several methods of migration regulation based on different mechanical stimuli have been reported. While vibrational stimuli, such as sound waves, show promise for regulating migration, the effect of the vibration direction on collective cell migration has not been studied in depth. Therefore, we fabricated a vibrating system that can apply horizontal vibration to a cell culture dish. Here, we evaluated the effect of the vibration direction on the collective migration of fibroblasts in a wound model comprising two culture areas separated by a gap. Results showed that the vibration direction affects the cell migration distance: vibration orthogonal to the gap enhances the collective cell migration distance while vibration parallel to the gap suppresses it. Results also showed that conditions leading to enhanced migration distance were also associated with elevated glucose consumption. Furthermore, under conditions promoting cell migration, the cell nuclei become elongated and oriented orthogonal to the gap. In contrast, under conditions that reduce the migration distance, cell nuclei were oriented to the direction parallel to the gap.

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Cell Patterning Method on a Clinically Ubiquitous Culture Dish Using Acoustic Pressure Generated From Resonance Vibration of a Disk-Shaped Ultrasonic Transducer

Cited by 17 publications

References 45 publications

Advances in Micromanipulation Actuated by Vibration-Induced Acoustic Waves and Streaming Flow

Advances in Micromanipulation Actuated by Vibration-Induced Acoustic Waves and Streaming Flow

Direct Cell Counting Using Macro-Scale Smartphone Images of Cell Aggregates

Collective cell migration of fibroblasts is affected by horizontal vibration of the cell culture dish

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