Cell Patterning by Laser-Assisted Bioprinting

Devillard, Raphaël; Pagès, E.; Correa, Manuela Medina; Kériquel, Virginie; Rémy, Murielle; Kalisky, Jérôme; Ali, M. Yakut; Guillotin, Bertrand; Guillemot, Fabien

doi:10.1016/b978-0-12-416742-1.00009-3

Cited by 69 publications

(44 citation statements)

References 49 publications

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“…For example, during droplet generation substantial energy is delivered to the sample leading to vibration, heating and/or strong electrical fields 12. Milder bioprinting methods are currently developed, such as the laser‐assisted bioprinting 14. In this method, a laser pulse locally melts a “bioribbon” that consists of a cell‐embedding gel, thus generating a droplet deposited with high precision according to the desired 3D pattern.…”

Section: Bioink‐based Bioprinting and Its Limitationsmentioning

confidence: 99%

Progress in scaffold‐free bioprinting for cardiovascular medicine

Moldovan

2018

J Cellular Molecular Medi

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Biofabrication of tissue analogues is aspiring to become a disruptive technology capable to solve standing biomedical problems, from generation of improved tissue models for drug testing to alleviation of the shortage of organs for transplantation. Arguably, the most powerful tool of this revolution is bioprinting, understood as the assembling of cells with biomaterials in three‐dimensional structures. It is less appreciated, however, that bioprinting is not a uniform methodology, but comprises a variety of approaches. These can be broadly classified in two categories, based on the use or not of supporting biomaterials (known as “scaffolds,” usually printable hydrogels also called “bioinks”). Importantly, several limitations of scaffold‐dependent bioprinting can be avoided by the “scaffold‐free” methods. In this overview, we comparatively present these approaches and highlight the rapidly evolving scaffold‐free bioprinting, as applied to cardiovascular tissue engineering.

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Section: Bioink‐based Bioprinting and Its Limitationsmentioning

confidence: 99%

Progress in scaffold‐free bioprinting for cardiovascular medicine

Moldovan

2018

J Cellular Molecular Medi

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“…Previous studies on LIFT systems used either solid receiver substrates when patterning non-sensitive material [21], or receiver slides coated with a thin layer of damping material such as Matrigel or gelatin to increase cell viability for bioprinting applications [23][24][25]. Here, to investigate direct three-dimensional liquid delivery, the receiver substrate consists of a 300 μm thick soft gelatin substrate.…”

Section: Lift For Direct Three-dimensional Liquid Deliverymentioning

confidence: 99%

Depth-controlled laser-induced jet injection for direct three-dimensional liquid delivery

et al. 2018

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Needle-free jet injection enables the delivery of drugs into skin or soft tissue by puncturing them with a high-velocity liquid jet. However, precise and efficient drug delivery requires generating such liquid jets with both a controlled velocity and a high throughput, which remains challenging with current spring-and gas-actuated jet injectors. Here, we propose a depth-controlled and high-throughput injection method by adapting laser-induced forward transfer (LIFT), a high-resolution two-dimensional printing technique, for direct three-dimensional liquid delivery into soft tissues.The velocity of thin liquid jets is laser actuated from 10 to 85 m/s so that doses as small as 10 pL, not achievable with other injectors, are injected at a 1 Hz repetition rate into a 300 μm thick soft gelatin substrate with a 25 μm depth precision and 12 μm lateral resolution. We further investigate the potential of this liquid delivery technique as a direct three-dimensional cell-delivery vehicle and show that depth-controlled particle delivery requires high-delivery efficiency. Our direct three-dimensional liquid delivery system opens up more possibilities for pinpoint drug delivery in soft tissues or tissue-engineered constructs.

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“…The laser assisted bio-printing [24], setup is composed of a Nd:YAG laser, a microscopic objective and a ribbon positioned onto an XYZ translation stage. Each layer of the 3D image represents the shape of the cross-section of the model at a specific level.…”

Section: Rapid Prototyping Technologymentioning

confidence: 99%

Biomaterial and Regenerative Medicine Approaches to Restoration of Flexion in Lumbar Herniated Disc

Leela¹

2016

Glob J Biotechnol Biomater Sci

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Objective: The systematic objective of this paper is to restore flexion in vertebral bone and to nourish the nucleus pulposus through the approach of biomaterial and regenerative medicine.Method: Studies were made on the treatment of lumbar herniated disc in CINAHIL, PubMed, EMBASE, Cochrane Central Register of Controlled Trials (CENTRAL) and Cochrane Database of Systematic Reviews(CDSR). This paper provides methods for restoration of flexion in the bone. A biomaterial scaffold made of poly-β-hydroxybutyrate (PHB) consisting of extracellular matrix (ECM) proteins which is synthesized in vitro through regenerative medicine is replaced in the place of degenerated disc. The scaffold model is designed in CAD and then printed in LAB. The metabolic functions of the disc are performed by the scaffold.Results: Fifteen papers, published between 2004 and 2014 were included in this study. Disc height restoration is possible using biomaterial and tissue engineering but none of the papers provide reports on flexion of the bone. The treatment of non-operative modalities like physical therapy, antiinflammatory medications and steroid injections still results in low back pain. The surgical methods like Joint arthroplasty and non-fusion techniques spare motion but not restore normal spine biomechanics. The study shows that the treatment with cells and biomaterials shows better results. Conclusion:Both the non-operative and surgical methods do not provide a good result. They do not offer flexion in the bone. The use of biomaterial scaffolds with ECM proteins paves a way for the medical professionals to treat lumbar herniated disc.

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Cell Patterning by Laser-Assisted Bioprinting

Cited by 69 publications

References 49 publications

Progress in scaffold‐free bioprinting for cardiovascular medicine

Progress in scaffold‐free bioprinting for cardiovascular medicine

Depth-controlled laser-induced jet injection for direct three-dimensional liquid delivery

Biomaterial and Regenerative Medicine Approaches to Restoration of Flexion in Lumbar Herniated Disc

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