Cell Orientation by a Microgrooved Substrate Can Be Predicted by Automatic Control Theory

Kemkemer, Ralf; Jungbauer, Simon; Kaufmann, Dieter; Gruler, Hans

doi:10.1529/biophysj.105.067967

Cited by 52 publications

(60 citation statements)

References 42 publications

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“…1A) (30,31). S = 0 corresponds to a random orientation, S = 1 corresponds to a perfect alignment with the main axis, and S = −1 corresponds to a perfect orientation perpendicular to the main axis.…”

Section: Resultsmentioning

confidence: 99%

Architecture and migration of an epithelium on a cylindrical wire

Yevick

Duclos

Bonnet

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

104

116

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In a wide range of epithelial tissues such as kidney tubules or breast acini, cells organize into bidimensional monolayers experiencing an out-of-plane curvature. Cancer cells can also migrate collectively from epithelial tumors by wrapping around vessels or muscle fibers. However, in vitro experiments dealing with epithelia are mostly performed on flat substrates, neglecting this out-ofplane component. In this paper, we study the development and migration of epithelial tissues on glass wires of well-defined radii varying from less than 1 μm up to 85 μm. To uncouple the effect of out-of-plane curvature from the lateral confinement experienced by the cells in these geometries, we compare our results to experiments performed on narrow adhesive tracks. Because of lateral confinement, the velocity of collective migration increases for radii smaller than typically 20 μm. The monolayer dynamics is then controlled by front-edge protrusions. Conversely, high curvature is identified as the inducer of frequent cell detachments at the front edge, a phenotype reminiscent of the Epithelial−Mesenchymal Transition. High curvature also induces a circumferential alignment of the actin cytoskeleton, stabilized by multiple focal adhesions. This organization of the cytoskeleton is reminiscent of in vivo situations such as the development of the trachea of the Drosophila embryo. Finally, submicron radii halt the monolayer, which then reconfigures into hollow cysts.collective cell behaviors | out-of-plane curvature | epithelial tubes

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Section: Resultsmentioning

confidence: 99%

Architecture and migration of an epithelium on a cylindrical wire

Yevick

Duclos

Bonnet

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

104

116

View full text Add to dashboard Cite

show abstract

“…In the simple case, a cell follows bipolar dilation with little lateral expansion (apart from a prototypical CHO cell, melanocytes would be a good example). In this case, it may be feasible to describe the response to various surface geometries in a dose-response fashion as successfully done by several authors [10,23]. One has to postulate that these cells possess a kind of "quorum sensing" mechanism which allows the existence of not more than two poles and supresses expansion in other directions.…”

Section: Discussionmentioning

confidence: 99%

“…It is also not clear if there is one uniform mechanism active over the entire range of scales which evoke a response, or if different processes accomplish guidance on the micro-and nanoscale, respectively. The extent of contact guidance depends on cell type used, plating density, and, most import, feature geometry and feature aspect ratio [6,[9][10][11][12][13]. In some instances, growth perpendicular to the groove direction has been observed [14,15], and the response of cells may depend on soluble factors [15].…”

Section: Introductionmentioning

confidence: 99%

Dynamics of Spreading and Alignment of Cells CulturedIn Vitroon a Grooved Polymer Surface

Peterbauer

Yakunin

Siegel

et al. 2011

Journal of Nanomaterials

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We used mechanically embossed polyester films to analyze the dynamics of cell alignment and cell-specific factors modulating the response of Chinese hamster ovary (CHO) cells and of a rat myogenic cell line to the surface topography. The films used had grooves with a periodicity of approximately 750 nm and a depth of 150 nm. Both cell lines responded to the topographical feature. On unpatterned control areas, cells of both lines showed a random distribution with orientation angles close to 45• . Both cell types exhibited an elongated morphology on the patterned surface. CHO cells typically showed bipolar spreading. Their contact area increased almost exclusively along the groove direction. Likewise, freshly seeded rat myoblasts displayed protrusions emerging in parallel with the grooves. However, myoblasts frequently had more than two sites with plasma protrusions pulling the cells along different grooves. They could also develop lamellipodia expanding without a preferred direction and long filopodia.

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“…by PBS and finally used in the experiments. The surface topography of the substrates was verified by atomic force microscopy and scanning electron microscopy [Kemkemer et al, 2006].…”

Section: Cell Culture Substratesmentioning

confidence: 99%

Partial Blindness to Submicron Topography in <b><i>NF1</i></b> Haploinsufficient Cultured Fibroblasts Indicates a New Function of Neurofibromin in Regulation of Mechanosensoric

Kaufmann

Hoesch

Su³

et al. 2012

Mol Syndromol

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Cells sense physical properties of their extracellular environment and translate them into biochemical signals. In this study, cell responses to surfaces with submicron topographies were investigated in cultured human NF1 haploinsufficient fibroblasts. Age-matched fibroblasts from 8 patients with neurofibromatosis type 1 (NF1^+/–) and 9 controls (NF1^+/+) were cultured on surfaces with grooves of 200 nm height and lateral distance of 2 µm. As cellular response indicator, the mean cell orientation along microstructured grooves was systematically examined. The tested NF1 haploinsufficient fibroblasts were significantly less affected by the topography than those from healthy donors. Incubation of the NF1^+/– fibroblasts with the farnesyltransferase inhibitor FTI-277 and other inhibitors of the neurofibromin pathway ameliorates significantly the cell orientation. These data indicate that NF1 haploinsufficiency results in an altered response to specific surface topography in fibroblasts. We suggest a new function of neurofibromin in the sensoric mechanism to topographies and a partial mechanosensoric blindness by NF1 haploinsufficiency.

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Cell Orientation by a Microgrooved Substrate Can Be Predicted by Automatic Control Theory

Cited by 52 publications

References 42 publications

Architecture and migration of an epithelium on a cylindrical wire

Architecture and migration of an epithelium on a cylindrical wire

Dynamics of Spreading and Alignment of Cells CulturedIn Vitroon a Grooved Polymer Surface

Partial Blindness to Submicron Topography in <b><i>NF1</i></b> Haploinsufficient Cultured Fibroblasts Indicates a New Function of Neurofibromin in Regulation of Mechanosensoric

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