Cell multiplication and blastoderm development in relation to egg envelope formation during uterine development of quail (<i>Coturnix coturnix japonica</i>) embryo

Stępińska, U.; Olszańska, Bożenna

doi:10.1002/jez.1402280310

Cited by 36 publications

(16 citation statements)

References 13 publications

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“…As demonstrated in the present study and by Stępinska and Olszańska [19], in natural conditions in the oviduct quail embryos developed to stages III-IV at 9-10 h, to stage V at 12 h, to stage VI at 13-14 h, to stages VII-VIII at 15-17 h, and to stages X-XI at 24 h after ovulation. Delay of embryo development in our ICSI experiment may be attributable at least in part to the high volume of PVP injected as well as mechanical damage to the germinal disc due to injection.…”

Section: Discussionsupporting

confidence: 49%

Fertilization and Development of Quail Oocytes after Intracytoplasmic Sperm Injection1

Hrabia

Takagi

Ono³

et al. 2003

Biology of Reproduction

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The present study was conducted to establish the intracytoplasmic sperm injection (ICSI) method for in vitro fertilization and development in quail. The efficiency of fertilization of oocytes was compared 1) between spontaneous and premature ovulation and 2) among testicular round spermatids, elongated spermatids, and immature and mature spermatozoa. The oocytes were injected with a single spermatozoon or spermatid and cultured for 24 h. Cell division was histologically observed with hematoxylin-eosin (HE) and a nucleus-specific fluorescent dye (DAPI). Five of 30 (16.6%) and 4 of 30 (13.3%) oocytes injected with mature sperm were fertilized in the spontaneous and induced ovulation group, respectively. Those embryos showed development at stages II-VII. Half the number (three of six) of the oocytes injected with testicular spermatozoa were fertilized and developed to stages IV-VII, and two of five oocytes injected with elongated spermatids were fertilized and developed to stage VI. All ooocytes injected with round spermatids were unfertilized. The results demonstrate that intracytoplasmic injection of a single sperm into quail oocyte can activate the oocyte and lead to fertilization. Oocytes prematurely ovulated are capable of fertilizing with mature sperm as are those spontaneously ovulated. In addition, the results suggest that the testicular round spermatids may not possess sufficient oocyte-activating potency but that the elongated spermatids and immature spermatozoa are competent to participate in fertilization and early embryonic development in quail.

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Section: Discussionsupporting

confidence: 49%

Fertilization and Development of Quail Oocytes after Intracytoplasmic Sperm Injection1

Hrabia

Takagi

Ono³

et al. 2003

Biology of Reproduction

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“…This RNA corresponds to the RNA contained in the blastodiscs, as we were not able to extract RNA from yolk. The amount of 2.1 pg/oocyte for the hen (Table 2) is found, logically, to be lower than in the blastoderm of a laid hen egg-3.26 pg (McMasters and Modak, 19771, since the latter contains about 4-5 x lo4 cells (Kochav et al, 1980;Stepinska and Olszanska, 1983) and where embryo RNA synthesis has already started during uterine development several hours before egg laying (Olszanska et al, 1984). The oocyte RNA content for hen, guinea hen, and turkey are quite similar, if not the same, and equal about 2 pg/oocyte, but that of quail seems to be about half that amount.…”

Section: Resultsmentioning

confidence: 99%

Maternal RNA content in oocytes of several mammalian and avian species

Olszańska

Borgul

1993

J. Exp. Zool.

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The amount of maternal RNA in the oocytes of several mammalian (mouse, rabbit, cow, pig, sheep) and avian (hen, Japanese quail, guinea hen, turkey) species was determined. For mammals these were in the range of 0.47 ng/oocyte (mouse) to 0.98 ng/oocyte (cow), with the exception of the rabbit, where it was up to 15 ng/oocyte. Birds' oocytes contain much higher levels of RNA, from 1.0 microgram (Japanese quail) up to 2.1 micrograms/oocyte (hen). The RNA levels were determined by direct measurement of EtBr-induced fluorescence of the oocyte homogenates without prior extraction and purification, and correlate well with standard spectrophotometric OD260 measurements. This method is useful when there are limited amounts of a sample, and it permits nucleic acid determination in the presence of proteins.

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“…The quail blastoderm from a freshly laid egg represents an embryo of about 40 000 cells (Stępińska & Olszańska, 1983) where transcription of all kinds of RNA (rRNA, poly(A+)RNA, 4-5 S RNA) has been observed (Olszań-ska et al, 1984). Thus the RNA from a germinal disc consists solely of maternal RNA accumulated during oogenesis, while the RNA from a blastoderm also contains in addition to the maternal RNA, the RNA synthesised by the embryo itself on maternal and paternal templates.…”

Section: Discussionmentioning

confidence: 98%

Expression of melatonin receptor transcripts (mel-1a, mel-1b and mel-1c) in Japanese quail oocytes and eggs

Oblap

Olszańska

2001

Zygote

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Cloning and sequencing of the cDNA for avian melatonin (MEL) receptors have made it possible to investigate the expression of these receptors in different animal tissues and organs by reverse transcription polymerase chain reaction. Our study demonstrates for the first time, the presence of MEL receptor transcripts in maternal RNA from Japanese quail oocytes and in RNA from the early embryos of the laid eggs. Specific primers permitted discrimination between mel-1a, mel-1b and mel-1c receptor sequences, and special techniques used to obtain the biological material made it possible to avoid accidental contamination with cells of somatic origin. Mel-1c transcript was the main one found in the oocytes and was constantly present in the maternal RNA from individual ovulated oocytes, while mel-1a and mel-1b transcripts were only sporadically present. All three MEL receptor transcripts appeared in the RNA from blastoderms of laid eggs, pointing to their de novo synthesis during uterine embryo development. The relative amount of the MEL receptor transcripts was low in relation to that of β-actin, roughly 3 × 10-4 the amount of control β-actin transcript. The presence of MEL receptor transcripts and their de novo synthesis in the 24-h-old bird embryos point to their probable wider distribution and earlier appearance in development than originally thought; the existence of the corresponding functional receptor proteins in such early embryos has not previously been reported.

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Cell multiplication and blastoderm development in relation to egg envelope formation during uterine development of quail (Coturnix coturnix japonica) embryo

Cited by 36 publications

References 13 publications

Fertilization and Development of Quail Oocytes after Intracytoplasmic Sperm Injection1

Fertilization and Development of Quail Oocytes after Intracytoplasmic Sperm Injection1

Maternal RNA content in oocytes of several mammalian and avian species

Expression of melatonin receptor transcripts (mel-1a, mel-1b and mel-1c) in Japanese quail oocytes and eggs

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