Cell morphology as a design parameter in the bioengineering of cell–biomaterial surface interactions

Frost, Oliver G.; Owji, Nazanin; Thorogate, Richard; Kyriakidis, Christos; Sawadkar, Prasad; Mordan, Nicola; Knowles, Jonathan C.; Lali, Ferdinand V.; García‐Gareta, Elena

doi:10.1039/d1bm01149a

Cited by 10 publications

(13 citation statements)

References 44 publications

(63 reference statements)

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“…A cell culture material that allows cell sheet formation and subsequent detachment by simply changing temperature is typically used [ 7 ]. Much research on 2D tissue engineering focuses on the investigation of optimized cell culture surfaces that promote cell growth and allow investigation of cellular phenomena in response to stimuli [ 11 , 46 , 47 , 48 ]. Finally, functional coatings are important to, for example, enhance osseointegration of metallic implants [ 41 , 43 ].…”

Section: Resultsmentioning

confidence: 99%

“…In the last decade, this original purpose has evolved towards the production of tissue analogues and models to study organ formation, tumor progression, or new drug therapies, among others [ 2 , 3 , 4 , 5 ]. These models can be 2D or 3D, including intelligent cell culture surfaces, organs-on-chip, organoids, or scaffold-based models [ 6 , 7 , 8 , 9 , 10 , 11 ]. One component remains key in their development: a biomaterial scaffold.…”

Section: Introductionmentioning

confidence: 99%

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Hydrocolloids of Egg White and Gelatin as a Platform for Hydrogel-Based Tissue Engineering

Pele,

Amaveda,

Mora

et al. 2023

Gels

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Innovative materials are needed to produce scaffolds for various tissue engineering and regenerative medicine (TERM) applications, including tissue models. Materials derived from natural sources that offer low production costs, easy availability, and high bioactivity are highly preferred. Chicken egg white (EW) is an overlooked protein-based material. Whilst its combination with the biopolymer gelatin has been investigated in the food technology industry, mixed hydrocolloids of EW and gelatin have not been reported in TERM. This paper investigates these hydrocolloids as a suitable platform for hydrogel-based tissue engineering, including 2D coating films, miniaturized 3D hydrogels in microfluidic devices, and 3D hydrogel scaffolds. Rheological assessment of the hydrocolloid solutions suggested that temperature and EW concentration can be used to fine-tune the viscosity of the ensuing gels. Fabricated thin 2D hydrocolloid films presented globular nano-topography and in vitro cell work showed that the mixed hydrocolloids had increased cell growth compared with EW films. Results showed that hydrocolloids of EW and gelatin can be used for creating a 3D hydrogel environment for cell studies inside microfluidic devices. Finally, 3D hydrogel scaffolds were fabricated by sequential temperature-dependent gelation followed by chemical cross-linking of the polymeric network of the hydrogel for added mechanical strength and stability. These 3D hydrogel scaffolds displayed pores, lamellae, globular nano-topography, tunable mechanical properties, high affinity for water, and cell proliferation and penetration properties. In conclusion, the large range of properties and characteristics of these materials provide a strong potential for a large variety of TERM applications, including cancer models, organoid growth, compatibility with bioprinting, or implantable devices.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hydrocolloids of Egg White and Gelatin as a Platform for Hydrogel-Based Tissue Engineering

Pele,

Amaveda,

Mora

et al. 2023

Gels

View full text Add to dashboard Cite

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“…The literature shows that cell morphology is a prime property of cells because changes in cellular function, including cell phenotype, cause morphological variations, and vice versa. 53 , 54 When osteogenic differentiation of stem cells occurs, the cellular morphology changes from the characteristic fibroblastic monolayer to a multilayered fibroblastic culture. As cells differentiate, the organization of cytoskeletal elements leads to these morphological changes.…”

Section: Resultsmentioning

confidence: 99%

A bone‐on‐a‐chip collagen hydrogel‐based model using pre‐differentiated adipose‐derived stem cells for personalized bone tissue engineering

Alamán‐Díez

García‐Gareta

Arruebo

et al. 2022

J Biomedical Materials Res

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Mesenchymal stem cells have contributed to the continuous progress of tissue engineering and regenerative medicine. Adipose‐derived stem cells (ADSC) possess many advantages compared to other origins including easy tissue harvesting, self‐renewal potential, and fast population doubling time. As multipotent cells, they can differentiate into osteoblastic cell linages. In vitro bone models are needed to carry out an initial safety assessment in the study of novel bone regeneration therapies. We hypothesized that 3D bone‐on‐a‐chip models containing ADSC could closely recreate the physiological bone microenvironment and promote differentiation. They represent an intermedium step between traditional 2D–in vitro and in vivo experiments facilitating the screening of therapeutic molecules while saving resources. Herein, we have differentiated ADSC for 7 and 14 days and used them to fabricate in vitro bone models by embedding the pre‐differentiated cells in a 3D collagen matrix placed in a microfluidic chip. Osteogenic markers such as alkaline phosphatase activity, calcium mineralization, changes on cell morphology, and expression of specific proteins (bone sialoprotein 2, dentin matrix acidic phosphoprotein‐1, and osteocalcin) were evaluated to determine cell differentiation potential and evolution. This is the first miniaturized 3D‐in vitro bone model created from pre‐differentiated ADSC embedded in a hydrogel collagen matrix which could be used for personalized bone tissue engineering.

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“…Since the cellular response depends on multiple components such as substrate stiffness, architecture, pore size, presence of fibers, alignment, etc., we believe that several/combinations of these are involved in invoking a specific outcome. We also established that cell morphology plays an important role in determining biological response . However, additional studies will be necessary to design effective implants, materials, and tissue engineering scaffolds to meet the need of an increasingly aged population.…”

Section: Discussionmentioning

confidence: 99%

“…We also established that cell morphology plays an important role in determining biological response. 84 However, additional studies will be necessary to design effective implants, materials, and tissue engineering scaffolds to meet the need of an increasingly aged population.…”

Section: Discussionmentioning

confidence: 99%

Cellular Senescence Program is Sensitive to Physical Differences in Polymeric Tissue Scaffolds

Yadav,

Shah,

Roy

et al. 2023

ACS Mater. Au

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A typical cellular senescence program involves exposing cells to DNA-damaging agents such as ionization radiation or chemotherapeutic drugs, which cause multipronged changes, including increased cell size and volume, the onset of enhanced oxidative stress, and inflammation. In the present study, we examined if the senescence onset decision is sensitive to the design, porosity, and architecture of the substrate. To address this, we generated a library of polymeric scaffolds widely used in tissue engineering of varied stiffness, architecture, and porosity. Using irradiated A549 lung cancer cells, we examined the differences between cellular responses in these 3D scaffold systems and observed that senescence onset is equally diminished. When compared to the two-dimensional (2D) culture formats, there were profound changes in cell size and senescence induction in three-dimensional (3D) scaffolds. We further establish that these observed differences in the senescence state can be attributed to the altered cell spreading and cellular interactions on these substrates. This study elucidates the role of scaffold architecture in the cellular senescence program.

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Cell morphology as a design parameter in the bioengineering of cell–biomaterial surface interactions

Abstract: Control of cell-surface interaction is necessary for biomaterial applications such as cell sheets, intelligent cell culture surfaces, or functional coatings. In this paper, we propose the emergent property of cell...

Cited by 10 publications

References 44 publications

Hydrocolloids of Egg White and Gelatin as a Platform for Hydrogel-Based Tissue Engineering

Hydrocolloids of Egg White and Gelatin as a Platform for Hydrogel-Based Tissue Engineering

A bone‐on‐a‐chip collagen hydrogel‐based model using pre‐differentiated adipose‐derived stem cells for personalized bone tissue engineering

Cellular Senescence Program is Sensitive to Physical Differences in Polymeric Tissue Scaffolds

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