Cell migration, viability and tissue reaction of calcium hypochlorite based-solutions irrigants: An in vitro and in vivo study

Blattes, Gabriela Bess Ferraz; Mestieri, Letícia Boldrin; Böttcher, Daiana Elisabeth; Fossati, Anna Christina Medeiros; Montagner, Francisco; Grecca, Fabiana Soares

doi:10.1016/j.archoralbio.2016.08.037

Cited by 30 publications

(35 citation statements)

References 22 publications

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“…Previous studies have also demonstrated that HSP27 protects against mitochondrial-induced apoptosis (15,16). Therefore the present study aimed to determine whether SPARC facilitates the suppression of HSP27-mediated protection of mitochondrial Δψ.…”

Section: Resultsmentioning

confidence: 85%

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SPARC overexpression suppresses radiation-induced HSP27 and induces the collapse of mitochondrial Δψ in neuroblastoma cells

et al. 2017

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Neuroblastoma is the cause of >15% of cancer-associated mortality in children in the USA. Despite aggressive treatment regimens, the long-term survival rate for these children remains at <40%. The current study demonstrates that secreted protein acidic and rich in cysteine (SPARC) suppresses radiation-induced expression of heat shock protein 27 (HSP27) in vivo and suppresses mitochondrial membrane potential (Δψ) in neuroblastoma cells. In the present study, the overexpression of SPARC in SK-N-BE(2) and NB1691 neuroblastoma cell lines suppresses radiation-induced G2M cell cycle arrest, proliferation, HSP27 expression (in vitro and in vivo) and induces the collapse of the mitochondrial Δψ. Gene ontology analysis demonstrated that the overexpression of SPARC combined with irradiation, induces the expression of dissimilar molecular function genes in SK-N-BE(2) and NB1691 cells, providing evidence of a dissimilar response signaling pathway. These results demonstrate that overexpression of SPARC suppresses radiation-induced HSP27 expression in neuroblastoma cells and the combination of SPARC and radiation induces the expression of protein 21, but suppresses neuroblastoma tumor density in in vivo mouse models. SPARC also induces mitochondrial Δψ collapse in SK-N-BE(2) and NB1691 neuroblastoma cells.

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Section: Resultsmentioning

confidence: 85%

“…The in vitro scratch assay was performed as previously described (16,17). Briefly, SK-N-BE(2) and NB1691 neuroblastoma cells were plated in 24-well plates (density, 10,000 cells/well).…”

Section: Methodsmentioning

confidence: 99%

SPARC overexpression suppresses radiation-induced HSP27 and induces the collapse of mitochondrial Δψ in neuroblastoma cells

et al. 2017

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“…The higher superficial tension of Ca(OCl) 2 can explain the results of present study, since this characteristic can affect the penetration of substance in the root canal walls. In addition to a similar antimicrobial activity, the Ca(OCl) 2 has also ability to promote tissue dissolution, it is a biocompatible substance , it maintains more stable the active chlorine content and does not promote reduction of the mechanical properties of dentin . For these reasons, the present study suggest the use of Ca(OCl) 2 as a viable alternative to be used as chemical auxiliary substance during the endodontic therapy.…”

Section: Discussionmentioning

confidence: 82%

“…Calcium hypochlorite (Ca[OCl] 2 ) is an alkaline white powder that was initially used for water treatment and industrial sterilisation . More recently, Ca(OCl) 2 has been found to be effective against E. faecalis , also promotes tissue dissolution , chemically stable in storage and biocompatible .…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of calcium and sodium hypochlorite in association with reciprocating instrumentation on decontamination of root canals infected with Enterococcus faecalis

et al. 2018

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The aim of this study was to evaluate the antimicrobial activity of sodium and calcium hypochlorite utilising reciprocating instrumentation. Sixty root canals were inoculated with E. faecalis for 14 days. Samples were divided into six groups according to decontamination protocol: G1: no treatment, G2: distilled water, G3: 2.5% sodium hypochlorite, G4: 2.5% calcium hypochlorite, G5: 5.25% sodium hypochlorite and G6: 5.25% calcium hypochlorite. Instrumentation was performed with Wave One reciprocating system (Dentsply Sirona Endodontics, York, PA, USA) in groups G2 to G6. Colony-forming units (CFUs) counting was performed and the data were subjected to Anova and Tukey (α = 0.05). Group 1 and 2 showed the highest mean contamination, with a significant difference between them (P < 0.05). Groups 3, 4, 5 and 6 showed the lowest contamination means with no significant difference between them (P < 0.05). Sodium and calcium hypochlorite, in association with reciprocating instrumentation, can be an effective decontamination protocol in root canals infected with E. faecalis.

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“…To overcome the problems associated with using NaOCl as an irrigant solution, Ca(OCl) 2 has been proposed as an alternative in endodontic therapy (14)(15)(16)25,26). It is very alkaline and tends to produce more chlorine than NaOCl (25), which partially explains its antimicrobial activity (16).…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Efficacy of Synthetic and Natural-Derived Novel Endodontic Irrigant Solutions

et al. 2018

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The aim of this study was to compare the efficacy of grape seed extract (GSE), calcium hypochlorite [Ca(ClO) 2 ], and sodium hypochlorite (NaOCl) irrigant solutions with rotary or reciprocating instrumentation for disinfection of root canals inoculated with Enterococcus faecalis. The mesiobuccal root canals of mandibular molars were prepared and inoculated with Enterococcus faecalis for 21 days. The roots were then randomly divided into the following eight experimental groups (n=11) according to the instrumentation technique and disinfection protocol: ProTaper Next or Reciproc R25 with sodium chloride (control group), 6% NaOCl, 6% Ca(ClO) 2 , or 50% GSE used for irrigation during instrumentation. The antimicrobial activity was determined on the basis of a reduction in colony-forming units (CFUs) counted on bacterial samples collected before and after root canal instrumentation and expressed as a percentage of reduction. Data were evaluated by two-way ANOVA followed by Tukey HSD post-hoc tests (p<0.05). No significant differences were observed in bacterial reduction between the ProTaper Next and Reciproc R25 systems (p>0.05), regardless of the irrigant solution used. Furthermore, all active solutions (6% NaOCl, 50% GSE, and 6% Ca(ClO) 2 ) showed similar potential to reduce bacterial counts (p>0.05) and were significantly more effective than sodium chloride (control) (p<0.05). The results suggest that the GSE and Ca(ClO) 2 have potential clinical application as irrigant solutions in endodontic therapy since they present bactericidal efficacy against Enterococcus faecalis.

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Cell migration, viability and tissue reaction of calcium hypochlorite based-solutions irrigants: An in vitro and in vivo study

Cited by 30 publications

References 22 publications

SPARC overexpression suppresses radiation-induced HSP27 and induces the collapse of mitochondrial Δψ in neuroblastoma cells

SPARC overexpression suppresses radiation-induced HSP27 and induces the collapse of mitochondrial Δψ in neuroblastoma cells

Effectiveness of calcium and sodium hypochlorite in association with reciprocating instrumentation on decontamination of root canals infected with Enterococcus faecalis

Antibacterial Efficacy of Synthetic and Natural-Derived Novel Endodontic Irrigant Solutions

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