Cell migration on material-driven fibronectin microenvironments

Grigoriou, Eleni; Cantini, Marco; Dalby, Matthew J.; Petersen, Ansgar; Salmerón-Sánchez, Manuel

doi:10.1039/c7bm00333a

Cited by 27 publications

(27 citation statements)

References 48 publications

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“…If the solution viscosity is too low, it can cause bead formation from the solution dispersing due to the electrostatic field [24]. For further investigation, the surface topography of the substrate was evaluated by using atomic force microscopy (AFM) [25,26]. The fiber stack in a nanofiber scaffold creates porosity.…”

Section: Nanofiber Scaffold Analysismentioning

confidence: 99%

Growth of Human Dermal Fibroblasts on Polyvinyl Alcohol-Silk Fibroin Nanofiber Scaffold

Giovanni¹,

Wibowo²,

Judawisastra³

et al. 2019

j.math.fund.sci.

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Skin tissue engineering is a developing technology to heal severe wounds. Combining polyvinyl alcohol (PVA) and silk fibroin (SF) nanofibers is a promising method of developing a skin scaffold because the resulting structure mimics collagen fibers. The aim of this research was to study the growth of human dermal fibroblasts (HDF) on a polyvinyl alcohol-silk fibroin (PVA-SF) nanofiber scaffold that was produced by electrospinning. Morphological characterization and chemical analysis of the scaffold were performed by scanning electron microscopy (SEM), Fourier transform infrared spectrophotometry (FTIR), and contact angle measurement. The biocompatibility of the scaffold was tested by MTT cytotoxicity assay, SEM analysis, adherence ratio calculation, and analysis of the HDF growth curve for 9 days. The FTIR results confirmed the presence of SF and PVA. The average fiber diameter and pore size of the PVA scaffold were greater than those of the PVA-SF scaffold. Both scaffolds had hydrophilic properties and were not cytotoxic. Thus, HDF can attach and grow on both types of scaffold better than HDF seeded on a polystyrene plate. In conclusion, the addition of SF to the PVA nanofibers caused bead formation, which affected the substrate topography, decreased hydrophilicity and also decreased the fiber diameter and pore size in the nanofiber scaffold compared to the PVA nanofiber scaffold without SF addition. SF addition increases cell attachment to the nanofiber scaffold and has potential to facilitate HDF cell growth.

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Section: Nanofiber Scaffold Analysismentioning

confidence: 99%

Growth of Human Dermal Fibroblasts on Polyvinyl Alcohol-Silk Fibroin Nanofiber Scaffold

Giovanni¹,

Wibowo²,

Judawisastra³

et al. 2019

j.math.fund.sci.

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“…17,49,107 Furthermore, previous studies have shown the importance of surface functionalization on cell culture. 108-110 While we have focused on the impact of protein functionalization of graphene – cell interfaces, further investigations are needed to better understand the time-dependent biochemical nature of such interfaces.…”

Section: Discussionmentioning

confidence: 99%

Prechondrogenic ATDC5 Cell Attachment and Differentiation on Graphene Foam; Modulation by Surface Functionalization with Fibronectin

Frahs

Reeck

Yocham

et al. 2019

ACS Appl. Mater. Interfaces

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Graphene foam holds promise for tissue engineering applications. In this study, graphene foam was used as a three-dimension scaffold to evaluate cell attachment, cell morphology, and molecular markers of early differentiation. The aim of this study was to determine if cell attachment and elaboration of an extracellular matrix would be modulated by functionalization of graphene foam with fibronectin, an extracellular matrix protein that cells adhere well to, prior to the establishment of three-dimensional cell culture. The molecular dynamic simulation demonstrated that the fibronectin-graphene interaction was stabilized predominantly through interaction between the graphene and arginine side chains of the protein. Quasi-static and dynamic mechanical testing indicated that fibronectin functionalization of graphene altered the mechanical properties of graphene foam. The elastic strength of the scaffold increased due to fibronectin, but the viscoelastic mechanical behavior remained unchanged. An additive effect was observed in the mechanical stiffness when the graphene foam was both coated with fibronectin and cultured with cells for 28 days. Cytoskeletal organization assessed by fluorescence microscopy demonstrated a fibronectin-dependent reorganization of the actin cytoskeleton and an increase in actin stress fibers. Gene expression assessed by quantitative real-time polymerase chain reaction of 9 genes encoding cell attachment proteins (Cd44,Ctnna1, Ctnnb1, Itga3, Itga5, Itgav, Itgb1, Ncam1, Sgce), 16 genes encoding extracellular matrix proteins (Col1a1, Col2a1, Col3a1, Col5a1, Col6a1, Ecm1, Emilin1, Fn1, Hapln1, Lamb3, Postn, Sparc, Spp1, Thbs1, Thbs2, Tnc), and 9 genes encoding modulators of remodeling (Adamts1, Adamts2, Ctgf, Mmp14, Mmp2, Tgfbi, Timp1, Timp2, Timp3) indicated that graphene foam provided a microenvironment conducive to expression of genes that are important in early chondrogenesis. Functionalization of graphene foam with fibronectin modified the cellular response to graphene foam, demonstrated by decreases in relative gene expression levels. These findings illustrate the combinatorial factors of microscale materials properties and nanoscale molecular features to consider in the design of three-dimensional graphene scaffolds for tissue engineering applications.

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“…These could act as scaffolds to present different growth factors or diffusible chemical signals to cells, on a controllable or stress-related way. Fibronectin, for example, has the ability to bind growth factors such as TGF-β or BMP-2 and keep them in a latent form to be presented to cells (Grigoriou et al 2017 ). Controllable stretching or degradation of these growth factor–bound fibres might not only change the mechanical properties but also causes release of signalling molecules causing them to present to cells in a physiologically relevant way.…”

Section: Ecm Architecture—ii Geometry: Confinement and Topographymentioning

confidence: 99%

Tissue engineering the cancer microenvironment—challenges and opportunities

2018

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Mechanosensing is increasingly recognised as important for tumour progression. Tumours become stiff and the forces that normally balance in the healthy organism break down and become imbalanced, leading to increases in migration, invasion and metastatic dissemination. Here, we review recent advances in our understanding of how extracellular matrix properties, such as stiffness, viscoelasticity and architecture control cell behaviour. In addition, we discuss how the tumour microenvironment can be modelled in vitro, capturing these mechanical aspects, to better understand and develop therapies against tumour spread. We argue that by gaining a better understanding of the microenvironment and the mechanical forces that govern tumour dynamics, we can make advances in combatting cancer dormancy, recurrence and metastasis.Electronic supplementary materialThe online version of this article (10.1007/s12551-018-0466-8) contains supplementary material, which is available to authorized users.

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Cell migration on material-driven fibronectin microenvironments

Abstract: Cell migration depends on the physical state of fibronectin, fibrillar vs. globular, which can be controlled by engineering biomaterials.

Cited by 27 publications

References 48 publications

Growth of Human Dermal Fibroblasts on Polyvinyl Alcohol-Silk Fibroin Nanofiber Scaffold

Growth of Human Dermal Fibroblasts on Polyvinyl Alcohol-Silk Fibroin Nanofiber Scaffold

Prechondrogenic ATDC5 Cell Attachment and Differentiation on Graphene Foam; Modulation by Surface Functionalization with Fibronectin

Tissue engineering the cancer microenvironment—challenges and opportunities

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