Cell migration guided by long-lived spatial memory

D’Alessandro, Joseph; Barbier-Chebbah, Alex; Cellerin, Victor; Bénichou, Olivier; Mège, René-Marc; Voituriez, Raphaël; Ladoux, Benoît

doi:10.1038/s41467-021-24249-8

Cited by 57 publications

(73 citation statements)

References 68 publications

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“…S3) PDMS microtubes. These observations suggested that CeR may not be sensitive to ECM components although substrate coating could be modified by cell-induced reorganization of the matrix ( 36 ).…”

Section: Resultsmentioning

confidence: 99%

The emergence of spontaneous coordinated epithelial rotation on cylindrical curved surfaces

et al. 2022

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Three-dimensional collective epithelial rotation around a given axis represents a coordinated cellular movement driving tissue morphogenesis and transformation. Questions regarding these behaviors and their relationship with substrate curvatures are intimately linked to spontaneous active matter processes and to vital morphogenetic and embryonic processes. Here, using interdisciplinary approaches, we study the dynamics of epithelial layers lining different cylindrical surfaces. We observe large-scale, persistent, and circumferential rotation in both concavely and convexly curved cylindrical tissues. While epithelia of inverse curvature show an orthogonal switch in actomyosin network orientation and opposite apicobasal polarities, their rotational movements emerge and vary similarly within a common curvature window. We further reveal that this persisting rotation requires stable cell-cell adhesion and Rac-1–dependent cell polarity. Using an active polar gel model, we unveil the different relationships of collective cell polarity and actin alignment with curvatures, which lead to coordinated rotational behavior despite the inverted curvature and cytoskeleton order.

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Section: Resultsmentioning

confidence: 99%

The emergence of spontaneous coordinated epithelial rotation on cylindrical curved surfaces

et al. 2022

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“…This class of self-interacting random walks has clear applications in a broad range of examples where a random walker modifies locally its environment -leaving behind footprints along its path, and in turn responds to its own footprints [28,29]. Such behaviours have been reported for ants depositing pheromones along their path [30], larger territorial animals [31], and have been identified quantitatively in the case of living cells that chemically modify and remodel the extra-cellular matrix [32,33].…”

Section: Introductionmentioning

confidence: 94%

“…The search process starts at t = 0, but the random walker is assumed to have explored the domain from t = −T to t = 0. S(t) is the survival probability of the random walker at time t. The scaling of S(t) with geometrical parameters is deduced from (30) and (32), for compact and non-compact processes respectively. The collapse of numerical simulations after rescaling captures the dependence of the FPT distribution on geometrical parameters.…”

Section: B Search Efficiency Of Self-interacting Random Walkersmentioning

confidence: 99%

Self-interacting random walks : aging, exploration and first-passage times

Barbier-Chebbah¹,

Bénichou²,

Voituriez³

2021

Preprint

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Self-interacting random walks are endowed with long range memory effects that emerge from the interaction of the random walker at time t with the territory that it has visited at earlier times t < t. This class of non Markovian random walks has applications in a broad range of examples, ranging from insects to living cells, where a random walker modifies locally its environment -leaving behind footprints along its path, and in turn responds to its own footprints. Because of their inherent non Markovian nature, the exploration properties of self-interacting random walks have remained elusive. Here we show that long range memory effects can have deep consequences on the dynamics of generic self-interacting random walks ; they can induce aging and non trivial persistence and transience exponents, which we determine quantitatively, in both infinite and confined geometries. Based on this analysis, we quantify the search kinetics of self-interacting random walkers and show that the distribution of the first-passage time (FPT) to a target site in a confined domain takes universal scaling forms in the large domain size limit, which we characterize quantitatively. We argue that memory abilities induced by attractive self-interactions provide a decisive advantage for local space exploration, while repulsive self-interactions can significantly accelerate the global exploration of large domains.

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“…1 To mimic natural cell distribution and to study cell migration and communication, the spatial control of cell-attachment (cell patterning) is of great interest. [2][3][4] The most frequently used approaches for cell-patterning focus on controlling the stiffness and texture of substrates or the position of cell attractive molecules on inorganic substrates or biomaterials. 2,[5][6][7][8] However, these methods usually lack spatiotemporal control of the morphology of the cellular environment, which plays an essential part in natural matrices that are constantly reshaped by various stimuli.…”

Section: Introductionmentioning

confidence: 99%

Photoinduced Amyloid Fibril Degradation for Controlled Cell Patterning

Kaygisiz

Ender

Gačanin

et al. 2022

Preprint

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Amyloid-like fibrils are a special class of self-assembling peptides that have emerged as a promising nanomaterial with rich bioactivity for applications such as cell adhesion and growth. Unlike the extracellular matrix, the intrinsically stable amyloid-like fibrils do not respond nor adapt to stimuli of their natural environment. Here, we designed a self-assembling motif (CKFKFQF), in which a photosensitive o-nitrobenzyl linker (PCL) was inserted. This peptide (CKFK-PCL-FQF) assembled into amyloid-like fibrils comparable to the unsubstituted CKFKFQF and revealed a strong response to UV-light. After UV irradiation, the secondary structure of the fibrils, fibril morphology and bioactivity were lost. Thus, coating surfaces with the pre-formed fibrils and exposing them to UV-light through a photomask generated well-defined areas with patterns of intact and destroyed fibrillar morphology. The unexposed, fibril-coated surface areas retained their ability to support cell adhesion in culture, in contrast to the light-exposed regions, where the cell-supportive fibril morphology was destroyed. Consequently, the photoresponsive peptide nanofibrils provide a facile and efficient way of cell patterning, exemplarily demonstrated for A549 cells. This study introduces photoresponsive amyloid-like fibrils as adaptive functional materials to precisely arrange cells on surface.

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Cell migration guided by long-lived spatial memory

Cited by 57 publications

References 68 publications

The emergence of spontaneous coordinated epithelial rotation on cylindrical curved surfaces

The emergence of spontaneous coordinated epithelial rotation on cylindrical curved surfaces

Self-interacting random walks : aging, exploration and first-passage times

Photoinduced Amyloid Fibril Degradation for Controlled Cell Patterning

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