Cell migration during heart regeneration in zebrafish

Abstract

Zebrafish possess the remarkable ability to regenerate injured hearts as adults, which contrasts the very limited ability in mammals. Although very limited, mammalian hearts do in fact have measurable levels of cardiomyocyte regeneration. Therefore, elucidating mechanisms of zebrafish heart regeneration would provide information of naturally occurring regeneration to potentially apply to mammalian studies, in addition to addressing this biologically interesting phenomenon in itself. Studies over the past 13 years have identified processes and mechanisms of heart regeneration in zebrafish. After heart injury, preexisting cardiomyocytes dedifferentiate, enter the cell cycle, and repair the injured myocardium. This process requires interaction with epicardial cells, endocardial cells, and vascular endothelial cells. Epicardial cells envelope the heart, while endocardial cells make up the inner lining of the heart. They provide paracrine signals to cardiomyocytes to regenerate the injured myocardium, which is vascularized during heart regeneration. In addition, accumulating results suggest that local migration of these major cardiac cell types have roles in heart regeneration. In this review, we summarize the characteristics of various heart injury methods used in the research community and regeneration of the major cardiac cell types. Then, we discuss local migration of these cardiac cell types and immune cells during heart regeneration.

ERBB2 drives YAP activation and EMT-like processes during cardiac regeneration

“…The emerging massive cooperation of ECM and cytoskeletal remodeling with CM migration seems to be pivotal for scar replacement by new CMs. This is in line with other studies suggesting that CM migration is essential for cardiac regeneration (Zhang et al, 2013) (Tahara et al, 2016) (Morikawa et al, 2015). infrastructure, which underlies their biology.…”

Investigation into the cellular origins of posterior regeneration in the annelid Capitella teleta

“…Cells involved in regeneration of new tissue are assumed to be derived from one or more of a number of different sources: by division of stem cells, by dedifferentiation and subsequent division of undifferentiated cells, or via transdifferentiation of pre‐existing cell types. In addition, migration of multiple cell types to the wound site has been reported in a diverse number of animals, such as cnidarians, planarians, zebrafish, and axolotls (Bradshaw, Thompson, & Frank, ; McCusker, Bryant, & Gardiner, ; Reddien & Alvarado, ; Tahara, Brush, & Kawakami, ).…”

“…Cells involved in regeneration of new tissue are assumed to be derived from one or more of a number of different sources: by division of stem cells, by dedifferentiation and subsequent division of undifferentiated cells, or via transdifferentiation of pre-existing cell types. In addition, migration of multiple cell types to the wound site has been reported in a diverse number of animals, such as cnidarians, planarians, zebrafish, and axolotls (Bradshaw, Thompson, & Frank, 2015;McCusker, Bryant, & Gardiner, 2015;Reddien & Alvarado, 2004;Tahara, Brush, & Kawakami, 2016). the description of neoblasts have been described mostly in clitellates, but also in some polychaetes (Bilello & Potswald, 1974;Cornec, Cresp, Delye, Hoarau, & Reynaud, 1987;Faulkner, 1929Faulkner, , 1932Krecker, 1923;Probst, 1931;Randolph, 1891Randolph, , 1892Stephan-Dubois, 1954;Stolte, 1929;Sugio et al, 2008;Tadokoro, Sugio, Kutsuna, Tochinai, & Takahashi, 2006;Zattara, Turlington, & Bely, 2016).…”

A Systematic Exposition of Methods used for Quantification of Heart Regeneration after Apex Resection in Zebrafish

“…However, a dissimilarity, beside a lack of pulmonary vasculature, is that the zebrafish have an absence of a coronary artery network that is found in the human heart. Instead, zebrafish have small coronary capillary like vessels localized to the compact muscle layer, which has to be taken into consideration when performing heart injury models [8]. In 2002, Poss et al [9] pioneered the field of zebrafish heart regeneration when demonstrating that the zebrafish heart fully regenerates following removal of~10-20% of the ventricular apex (AR) ( Figure 1A-C).…”