Cell migration and invasion assays

Valster, Aline H.; Tran, Nhan L.; Nakada, Mitsutoshi; Berens, Michael E.; Chan, Alice; Symons, Marc

doi:10.1016/j.ymeth.2005.08.001

Cited by 281 publications

(230 citation statements)

References 33 publications

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“…Inhibition of MCF-7 cell migration in vitro by A3 indicates that the extract could probably inhibit cell metastasis. 32 Treatment of MCF-7 cells with A3 resulted in a potent inhibition of cell migration toward the closure of the wound scratch, even after 12 and 18 h, indicating that the extract has a capability to suppress the motility of the breast cancer cells, whereas the wound was totally closed after 24 h in untreated cells. Cell invasion is one of the main hallmarks of metastatic cascade.…”

Section: Discussionmentioning

confidence: 93%

Proapoptotic and Antimetastatic Properties of Supercritical CO₂ Extract of Nigella sativa Linn. Against Breast Cancer Cells

Baharetha

Nassar²,

Aisha³

et al. 2013

Journal of Medicinal Food

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Nigella sativa, commonly referred as black cumin, is a popular spice that has been used since the ancient Egyptians. It has traditionally been used for treatment of various human ailments ranging from fever to intestinal disturbances to cancer. This study investigated the apoptotic, antimetastatic, and anticancer activities of supercritical carbon dioxide (SC-CO2) extracts of the seeds of N. sativa Linn. against estrogen-dependent human breast cancer cells (MCF-7). Twelve extracts were prepared from N. sativa seeds using the SC-CO2 extraction method by varying pressure and temperature. Extracts were analyzed using FTIR and UV-Vis spectrometry. Cytotoxicity of the extracts was evaluated on various human cancer and normal cell lines. Of the 12 extracts, 1 extract (A3) that was prepared at 60°C and 2500 psi (*17.24 MPa) showed selective antiproliferative activity against MCF-7 cells with an IC50 of 53.34 -2.15 lg/mL. Induction of apoptosis was confirmed by evaluating caspases activities and observing the cells under a scanning electron microscope. In vitro antimetastatic properties of A3 were investigated by colony formation, cell migration, and cell invasion assays. The elevated levels of caspases in A3 treated MCF-7 cells suggest that A3 is proapoptotic. Further nuclear condensation and fragmentation studies confirmed that A3 induces cytotoxicity through the apoptosis pathway. A3 also demonstrated remarkable inhibition in migration and invasion assays of MCF-7 cells at subcytotoxic concentrations. Thus, this study highlights the therapeutic potentials of SC-CO2 extract of N. sativa in targeting breast cancer.

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Section: Discussionmentioning

confidence: 93%

Proapoptotic and Antimetastatic Properties of Supercritical CO₂ Extract of Nigella sativa Linn. Against Breast Cancer Cells

Baharetha

Nassar²,

Aisha³

et al. 2013

Journal of Medicinal Food

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“…Migrated cells were stained with 0.1% crystal violet for 10 minutes at room temperature and examined by light microscopy. Quantification of invaded cells was conducted according to published criteria (22).…”

Section: Matrigel Invasion Assaymentioning

confidence: 99%

miRNA-145 Targets v-ets Erythroblastosis Virus E26 Oncogene Homolog 1 to Suppress the Invasion, Metastasis, and Angiogenesis of Gastric Cancer Cells

Zheng

et al. 2013

Molecular Cancer Research

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Recent evidence shows that v-ets erythroblastosis virus E26 oncogene homolog 1 (Ets1) is implicated in tumor development and progression. However, the clinical potentials and underlying mechanisms of Ets1 in gastric cancer progression and metastasis remain largely unknown. In this study, Ets1 immunostaining was identified in 56 of 84 (66.7%) gastric cancer tissues, which was correlated with tumor invasion and metastasis. In gastric cancer specimens and cell lines, miRNA-145 (miR-145) was downregulated and inversely correlated with Ets1 expression. Bioinformatics analysis and luciferase reporter assay revealed that miR-145 directly targeted the 3 0 -untranslated region (3 0 -UTR) of Ets1 mRNA. Overexpression or knockdown of miR-145 responsively altered both the mRNA and protein levels of Ets1 and its downstream genes, matrix metalloproteinase (MMP-1)-1 and -9, in gastric cancer cell lines SGC-7901 and MKN-45. Ectopic expression of miR-145 suppressed the invasion, metastasis, and angiogenesis of SGC-7901 and MKN-45 cells in vitro and in vivo. In addition, the effects of miR-145 on Ets1 expression, migration, invasion, and angiogenesis were rescued by restoration of Ets1 expression in these cells. Furthermore, anti-miR-145 inhibitor promoted the migration, invasion, and angiogenesis, whereas siRNAmediated Ets1 knockdown phenocopied the effects of miR-145 overexpression in gastric cancer cells. These results show that miR-145 suppresses Ets1 expression via the binding site in the 3 0 -UTR, thus inhibiting the invasion, metastasis, and angiogenesis of gastric cancer cells. Mol Cancer Res; 11(2); 182-93. Ó2012 AACR.

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“…Cell invasion and migration are important in tumor metastasis and angiogenesis [17]. Therefore, we wanted to determine whether ectopic expression of miR-21 affects the ability of cancer cells to invade and migrate.…”

Section: Effect Of Mir-21 On Invasion Migration and Vascular Tube Fmentioning

confidence: 99%

“…For instance, overexpression of miR-21 in human breast cancer is highly related to advanced tumor stage, lymph node metastasis, and poor patient prognosis [4]. In humans, the pre-miR-21 gene, MIRN21, is located on chromosome 17. Functional studies have demonstrated that miR-21 is a key molecule that turns on various cell signaling pathways in cancer [5].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-21 inhibitor potentiates anti-tumor effect of radiation therapy in vitro and in vivo

Cho¹,

Kim²,

Lee³

et al. 2014

Tumor Microenvironment and Therapy

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MicroRNA-21 (miR-21) plays important roles in carcinogenesis and is highly expressed in diverse human cancers. We evaluated the potential of targeting miRNA-21 to overcome the radioresistance of human cancer cells having an activated EGFR2-associated signaling and also aimed to elucidate the mechanisms of radiosensitization, and the effect on epithelial- mesenchymal transition (EMT). Ectopic overexpression of miR-21 up-regulated EGFR/HER2-associated signaling and increased radioresistance of a panel of human cancer cells (U251, U87, and A549 cells). In contrast, a specific inhibitor of miR-21 attenuated this signaling and radiosensitized a panel of human cancer cells. Inhibition of miR-21 was associated with persistent γH2AX foci formation. Inhibition of miR-21 decreased the typical features of EMT, such as invasion and migration and vascular tube formation. Treatment with anti-miR-21 decreased tumor burden in nude mice bearing intracranial U251 xenografts compared to controls. Combined treatment of anti-miR-21 and radiation further decreased tumor burden compared to each treatment alone. In summary, miR-21 is an important onco-miR, which confers radioresistance and diverse features of EMT. Inhibition of miR-21 could be a potential strategy for improving the efficacy of radiation therapy via unique modulation of pro-survival signaling implicated in radiation response and EMT.

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Cell migration and invasion assays

Cited by 281 publications

References 33 publications

Proapoptotic and Antimetastatic Properties of Supercritical CO₂ Extract of Nigella sativa Linn. Against Breast Cancer Cells

Proapoptotic and Antimetastatic Properties of Supercritical CO₂ Extract of Nigella sativa Linn. Against Breast Cancer Cells

miRNA-145 Targets v-ets Erythroblastosis Virus E26 Oncogene Homolog 1 to Suppress the Invasion, Metastasis, and Angiogenesis of Gastric Cancer Cells

MicroRNA-21 inhibitor potentiates anti-tumor effect of radiation therapy in vitro and in vivo

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Cell migration and invasion assays

Cited by 281 publications

References 33 publications

Proapoptotic and Antimetastatic Properties of Supercritical CO2 Extract of Nigella sativa Linn. Against Breast Cancer Cells

Proapoptotic and Antimetastatic Properties of Supercritical CO2 Extract of Nigella sativa Linn. Against Breast Cancer Cells

miRNA-145 Targets v-ets Erythroblastosis Virus E26 Oncogene Homolog 1 to Suppress the Invasion, Metastasis, and Angiogenesis of Gastric Cancer Cells

MicroRNA-21 inhibitor potentiates anti-tumor effect of radiation therapy in vitro and in vivo

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Proapoptotic and Antimetastatic Properties of Supercritical CO₂ Extract of Nigella sativa Linn. Against Breast Cancer Cells

Proapoptotic and Antimetastatic Properties of Supercritical CO₂ Extract of Nigella sativa Linn. Against Breast Cancer Cells