Cell metabolomics study on the anticancer effects of Ophiopogon japonicus against lung cancer cells using UHPLC/Q-TOF-MS analysis

Abstract

Ophiopogon japonicus (OJ) is a traditional Chinese herbal medicine that has been used for thousands of years. Recently, the anticancer effects of OJ have been reported in multiple types of cancer, particularly in lung cancer. However, the underlying mechanisms remain unclear. In present study, the effects of OJ against NCI-H1299 human lung cancer cells were investigated, and the underlying mechanisms were explored using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS)-based cell metabolomics. As a result, OJ inhibited the proliferation, induced the apoptosis and suppressed the migration of NCI-H1299 cells. A total of 22 differential metabolites responsible for the effects of OJ were screened and annotated based on the LC-MS-based cell metabolomics approach. The altered metabolites were involved in three metabolic pathways, including glycerophospholipid metabolism, ether lipid metabolism and glutathione metabolism. These results showed that cell metabolomics-based strategies are promising tools to discover the action mechanisms of OJ against lung cancer cells.

Elucidating the mechanism of Hongjinshen decoction in the treatment of pulmonary fibrosis based on network pharmacology and molecular docking

“…[14] In addition, another study found that Ophiopogon japonicus and Salvia miltiorrhiza may have a delayed effect on the development of pulmonary fibrosis by activating superoxide dismutase, inhibiting the scavenging of free radicals, improving the balance between oxidation and antioxidation, reducing cell membrane damage in lung tissue, and weakening the inflammatory response. [15] Fibrosis is scarring and tissue sclerosis caused by excessive deposition of extracellular matrix proteins by myofibroblasts in response to chronic inflammation. [16] A variety of noxious stimuli (including toxins, infectious agents, autoimmune responses, and mechanical stress) can induce fibroblast responses.…”

“…[ 14 ] In addition, another study found that Ophiopogon japonicus and Salvia miltiorrhiza may have a delayed effect on the development of pulmonary fibrosis by activating superoxide dismutase, inhibiting the scavenging of free radicals, improving the balance between oxidation and antioxidation, reducing cell membrane damage in lung tissue, and weakening the inflammatory response. [ 15 ]…”