Cell membrane electroporation-Part 2: the applications

Haberl, Saša; Miklavčič, Damijan; Serša, Gregor; Frey, Wolfgang; Rubinsky, Boris

doi:10.1109/mei.2013.6410537

Cited by 113 publications

(60 citation statements)

References 71 publications

(78 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…First, defects (water wires) penetrate the hydrophobic core of the bilayers; then, these expand to form water-filled pores spanning the whole bilayer, which are later stabilized by reorganization of lipid molecules (Levine and Vernier 2010;Tarek 2005;Tieleman 2004). Cell electroporation is used in several fields, like biology, biotechnology and medicine (Haberl et al 2013). Electroporation is considered reversible if cells recover their initial state after the electric field is switched off; electroporation is considered irreversible if it leads to cell death.…”

Section: Introductionmentioning

confidence: 99%

On the Electroporation Thresholds of Lipid Bilayers: Molecular Dynamics Simulation Investigations

et al. 2013

View full text Add to dashboard Cite

Electroporation relates to the cascade of events that follows the application of high electric fields and that leads to cell membrane permeabilization. Despite a wide range of applications, little is known about the electroporation threshold, which varies with membrane lipid composition. Here, using molecular dynamics simulations, we studied the response of dipalmitoyl-phosphatidylcholine, diphytanoyl-phosphocholine-ester and diphytanoylphosphocholine-ether lipid bilayers to an applied electric field. Comparing between lipids with acyl chains and methyl branched chains and between lipids with ether and ester linkages, which change drastically the membrane dipole potential, we found that in both cases the electroporation threshold differed substantially. We show, for the first time, that the electroporation threshold of a lipid bilayer depends not only on the ''electrical'' properties of the membrane, i.e., its dipole potential, but also on the properties of its component hydrophobic tails.

show abstract

Section: Introductionmentioning

confidence: 99%

On the Electroporation Thresholds of Lipid Bilayers: Molecular Dynamics Simulation Investigations

et al. 2013

View full text Add to dashboard Cite

show abstract

“…However, intentional transient permeabilization of the membrane is also exploited as a means to introduce genes or drugs into an organism, and targeted permanent disruption of plasma membranes is an effective means to eliminate specific cells (1)(2)(3).…”

mentioning

confidence: 99%

Imaging the dynamics of individual electropores

Sengel

Wallace

2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Electroporation is a widely used technique to permeabilize cell membranes. Despite its prevalence, our understanding of the mechanism of voltage-mediated pore formation is incomplete; methods capable of visualizing the time-dependent behavior of individual electropores would help improve our understanding of this process. Here, using optical single-channel recording, we track multiple isolated electropores in real time in planar droplet interface bilayers. We observe individual, mobile defects that fluctuate in size, exhibiting a range of dynamic behaviors. We observe fast (25 s −1 ) and slow (2 s −1 ) components in the gating of small electropores, with no apparent dependence on the applied potential. Furthermore, we find that electropores form preferentially in the liquid disordered phase. Our observations are in general supportive of the hydrophilic toroidal pore model of electroporation, but also reveal additional complexity in the interactions, dynamics, and energetics of electropores.droplet interface bilayer | electroporation | toroidal pores | optical single-channel recording | lipid bilayers

show abstract

“…As for many other cell factories, extraction of proteins is diminished by the cell wall barrier. Optimization of algal cell disruption methods is fundamental from an economic point of view [24]. Pulsed electric fields are now recognized as an efficient tool for Biotechnological processing [25].…”

Section: Protein Electroextraction From Microalgaementioning

confidence: 99%

Conductance Dynamics to Monitor and Control Cell Electropermeabilization

Teissié

2016

IFMBE Proceedings

View full text Add to dashboard Cite

Abstract-Membrane electropermeabilization is observed when electric pulses over a critical strength are delivered on a cell suspension. This is associated with a time dependent change in the suspension conductance. The magnitude and the kinetics of the conductivity changes can be used to get access to the molecular event supporting the membrane electropermeabilization. In Bioprocesses, where extraction results from electropermeabilization, the control of the conductivity change of the cell suspension allows the definition of optimized safe conditions.

show abstract

Cell membrane electroporation-Part 2: the applications

Abstract: Electroporation can be used as a tool for extracting or introducing molecules from or into a cell. The most important and promising applications of electroporation in medicine and biotechnology are described.

Cited by 113 publications

References 71 publications

On the Electroporation Thresholds of Lipid Bilayers: Molecular Dynamics Simulation Investigations

On the Electroporation Thresholds of Lipid Bilayers: Molecular Dynamics Simulation Investigations

Imaging the dynamics of individual electropores

Conductance Dynamics to Monitor and Control Cell Electropermeabilization

Contact Info

Product

Resources

About