Cell membrane disintegration and extracellular vesicle release in a model of different size and charge PAMAM dendrimers effects on cultured endothelial cells

Patel, Mehulkumar; Paoli, Silvia H. De; Elhelu, Oumsalama K; Farooq, Sehrish; Šimák, Jan

doi:10.1080/17435390.2019.1570373

Cited by 13 publications

(18 citation statements)

References 51 publications

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“…So, the HUVECs are one of the most popular cell models in cytotoxicity test. [40][41][42][43] Moreover, the U87 cells is one of the typical and common glioblastoma cell lines, and it was used as model cell in section of "Live Imaging" in vivo, and here, the U87 cells were served as model cell in the cytotoxicity evaluation. It is well known that macrophages are the most important type of sentinel cells, as the first defense line against invading pathogens and nanoparticles.…”

Section: On Integrity Of Cell Membrane By Lactate Dehydrogenase (Ldh)mentioning

confidence: 99%

The Endocytic Mechanism and Cytotoxicity of Boron-Containing Vesicles

et al. 2020

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A novel polymer (PEG 2000-carborane), self-assembling into spherical vesicles (boron-containing vesicles, BCVs), could be quickly taken up by tumor cells and had an enhance stability in the bloodstream in previous study. To have more comprehensive understanding of BCVs, endocytic mechanism and cytotoxicity assessment were conducted. The results showed that BCVs were taken up in the intact form with cholesteroldependent pathway during endocytosis, and BCVs exhibited nearly no cytotoxicity. BCVs could accumulate within tumors for at least 24 h. The data would provide reference information and guidance for BCVs' multifunctional application serving as a boron delivery agent for boron neutron capture therapy (BNCT), a hydrophilic and/or hydrophobic drug carrier and a diagnostic imaging fluorescent probe.

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Section: On Integrity Of Cell Membrane By Lactate Dehydrogenase (Ldh)mentioning

confidence: 99%

The Endocytic Mechanism and Cytotoxicity of Boron-Containing Vesicles

et al. 2020

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“…These results indicate that toxic effects of Ag@ZnO NPs on melanoma cells are primarily related to their potency to produce ROS under UV irradiation. The UV-induced ROS generation leads to oxidative stress that activates signalling pathways triggering apoptosis 36 . By this method, we are able to target the lesion area on the skin by focusing the irradiation.…”

Section: Uv Irradiation Amplifies the Cytotoxicity Of Ag@zno In Melanmentioning

confidence: 99%

Supramolecular Insights into Domino Effects of Ag@ZnO-Induced Oxidative Stress in Melanoma Cancer Cells

Ghaemi

Moshiri

Herrmann

et al. 2019

ACS Appl. Mater. Interfaces

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Recent studies suggest that cancer cell death accompanied by organelle dysfunction might be a promising approach for cancer therapy. The Golgi apparatus has a key role in cell function and may initiate signaling pathways to mitigate stress and, if irreparable, start apoptosis. It has been shown that Golgi disassembly and fragmentation under oxidative stress act as indicator for stress-mediated cell death pathways through cell cycle arrest in the G2/M phase. The present study shows that UV-induced reactive oxygen species (ROS) generation by Ag@ZnO nanoparticles (NPs) transform the Golgi structures from compressed perinuclear ribbons into detached vesicle-like structures distributed in the entire cytoplasm of melanoma cells. This study also demonstrates that Ag@ZnO NPs-induced Golgi fragmentation co-occurs with G2 block of the cell-cycle progression, preventing cells from entering the mitosis phase. Additionally, the increased intracellular ROS production triggered by Ag@ZnO NPs upon UV exposure, promoted autophagy. Taken together, Ag@ZnO NPs induce stress-related Golgi fragmentation and autophagy, finally leading to melanoma cell apoptosis. Intracellular oxidative stress generated by Ag@ZnO NPs upon UV irradiation may thus represent a targeted approach to induce cancer cell death through organelle destruction in melanoma cells while fibroblast cells remained significantly unaffected.

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“…Conditions such as hypoxia, irradiation, inflammation, oxidative stress-induced injury and shear stress can lead to the formation and release of endothelial EVs [ 9 ]. In BCBM formation, the interaction of BC cells (BCCs) with BMECs was shown to induce endothelial blebbing [ 18 ], a phenomenon that can be associated with endothelial EV release [ 19 ]. Thus, the BBB assumes a key role in BCBM, raising the hypothesis that it can constitute a source of biomarkers.…”

Section: Introductionmentioning

confidence: 99%

MicroRNAs and Extracellular Vesicles as Distinctive Biomarkers of Precocious and Advanced Stages of Breast Cancer Brain Metastases Development

Figueira

Godinho-Pereira

Galego

et al. 2021

IJMS

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Triple negative breast cancer presents higher mortality and poorer survival rates than other breast cancer (BC) types, due to the proneness to brain metastases formation, which are usually diagnosed at advanced stages. Therefore, the discovery of BC brain metastases (BCBM) biomarkers appears pivotal for a timely intervention. With this work, we aimed to disclose microRNAs (miRNAs) and extracellular vesicles (EVs) in the circulation as biomarkers of BCBM formation. Using a BCBM animal model, we analyzed EVs in plasma by nanoparticle tracking analysis and ascertained their blood-brain barrier (BBB) origin by flow cytometry. We further evaluated circulating miRNAs by RT-qPCR and their brain expression by in situ hybridization. In parallel, a cellular model of BCBM formation, combining triple negative BC cells and BBB endothelial cells, was used to differentiate the origin of biomarkers. Established metastases were associated with an increased content of circulating EVs, particularly of BBB origin. Interestingly, deregulated miRNAs in the circulation were observed prior to BCBM detection, and their brain origin was suggested by matching alterations in brain parenchyma. In vitro studies indicated that miR-194-5p and miR-205-5p are expressed and released by BC cells, endothelial cells and during their interaction. These results highlight miRNAs and EVs as biomarkers of BCBM in early and advanced stages, respectively.

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Cell membrane disintegration and extracellular vesicle release in a model of different size and charge PAMAM dendrimers effects on cultured endothelial cells

Cited by 13 publications

References 51 publications

The Endocytic Mechanism and Cytotoxicity of Boron-Containing Vesicles

The Endocytic Mechanism and Cytotoxicity of Boron-Containing Vesicles

Supramolecular Insights into Domino Effects of Ag@ZnO-Induced Oxidative Stress in Melanoma Cancer Cells

MicroRNAs and Extracellular Vesicles as Distinctive Biomarkers of Precocious and Advanced Stages of Breast Cancer Brain Metastases Development

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