Cell membrane array fabrication and assay technology

The lipid bilayer is one of the most eloquent and important self-assembled structures in nature. It not only provides a protective container for cells and sub-cellular compartments, but also hosts much of the machinery for cellular communication and transport across the cell membrane. Solid supported lipid bilayers provide an excellent model system for studying the surface chemistry of the cell. Moreover, they are accessible to a wide variety of surface-specific analytical techniques. This makes it possible to investigate processes such as cell signaling, ligand-receptor interactions, enzymatic reactions occurring at the cell surface, as well as pathogen attack. In this review, the following membrane systems are discussed: black lipid membranes, solid supported lipid bilayers, hybrid lipid bilayers, and polymer cushioned lipid bilayers. Examples of how supported lipid membrane technology is interfaced with array based systems by photolithographic patterning, spatial addressing, microcontact printing, and microfluidic patterning are explored. Also, the use of supported lipid bilayers in microfluidic devices for the development of lab-on-a-chip based platforms is examined. Finally, the utility of lipid bilayers in nanotechnology and future directions in this area are discussed.

show abstract

“…13) [14]. Additional methods include microcontact printing [134,144,145], laminar flow deposition [18,146], and robotic pin printing [147].…”

Section: Arrays Of Supported Phospholipid Membranes and Microfluidic mentioning

confidence: 99%

“…13b shows a brightfield image of the addressing process. This method has been expanded by employing quill pen printing and robotic addressing in a humidity controlled environment [147].…”

Section: Arrays Of Supported Phospholipid Membranes and Microfluidic mentioning

confidence: 99%

Solid supported lipid bilayers: From biophysical studies to sensor design

Castellana

Cremer

2006

Surface Science Reports

999

1,027

View full text Add to dashboard Cite

show abstract

“…The development of bilayer lipid membrane (BLM) sensor platforms is a promising technology for investigating and exploiting biological sensing mechanisms (Yamazaki et al, 2005). BLM based biosensors attempt to reproduce the natural environment of a membrane protein, providing similar membrane composition and fluidity, whilst positioning the protein between two aqueous compartments.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic array platform for simultaneous lipid bilayer membrane formation

Zagnoni

Sandison

Morgan

2009

Biosensors and Bioelectronics

View full text Add to dashboard Cite

“…Other applications might include enzyme patterning and characterization, 30,49 cell-supported bilayer interactions, 63 as well as microfluidic drug discovery platforms. 26 Now that spatially addressed bilayer arrays have been combined with microfluidics, there are three general goals that need to be pursued in association with this analytical methodology. First, the array size could be expanded by increasing the field of view of the fluorescence microscope.…”

Section: Discussionmentioning

confidence: 99%

Multiplexing Ligand−Receptor Binding Measurements by Chemically Patterning Microfluidic Channels

2008

View full text Add to dashboard Cite

A method has been designed for patterning supported phospholipid bilayers (SLBs) on planar substrates and inside microfluidic channels. To do this, bovine serum albumin (BSA) monolayers were formed via adsorption at the liquid/solid interface. Next, this interfacial protein film was selectively patterned by using deep UV lithography. Subsequently, SLBs could be deposited in the patterned locations by vesicle fusion. By cycling through this process several times, spatially addressed bilayer arrays could be formed with intervening protein molecules serving as twodimensional corrals. By employing this method, phospholipid bilayers containing various concentrations of ganglioside GM1 were addressed along the length of individual microfluidic channels. Therefore, the binding of GM1 with pentameric cholera toxin B (CTB) subunits could be probed. A seven-channel microfluidic device was fabricated for this purpose. Each channel was simultaneously patterned with four chemically distinct SLBs containing 0, 0.2, 0.5, and 2.0 mol % GM1, respectively. Varying concentrations of CTB were then introduced into each of the channels. With the use of total internal reflection fluorescence microscopy, it was possible to simultaneously abstract multiple equilibrium dissociation constants as a function of ligand density for the CTB-GM1 system in a single shot.Multivalent ligand-receptor interactions are ubiquitous on cell surfaces. They have a wide variety of consequences including the modulation of equilibrium dissociation constants, high receptor selectivity, and receptor clustering. 1 Multivalency can also play a direct role in signal transduction processes. 2 Examination of the underlying thermodynamics of ligandreceptor binding may lead to a greater understanding of its biological role and could provide insight into biomedical applications involving inhibitory drug design. 1,3,4 Unfortunately, high-throughput, low-protein consumption assays are not presently well enough developed in this field to afford rapid, systematic studies of binding data at two-dimensionally fluid bilayer interfaces. 5 In previous work it has been demonstrated that microfluidic devices can be designed for measuring binding affinities in multivalent systems. [5][6][7][8][9][10][11][12][13][14] In our previous setup, ligands were incorporated into supported lipid bilayers (SLBs) coated on the walls and floors of polydimethylsiloxane/glass microchannels. Linear arrays of channels were then monitored by total internal reflection fluorescence microscopy (TIRFM) 15 to obtain equilibrium dissociation constants in one-shot assays. This could be done by using the same surface chemistry in each microchannel while varying the solution concentration of the aqueous proteins. Such a setup made these assays relatively rapid, afforded high accuracy, and used only a few microliters of protein solution. Nevertheless, such platforms could be markedly improved by measuring multiple binding constants with various membrane chemistries simultaneously. This could be achie...

show abstract

Cell membrane array fabrication and assay technology

Cited by 56 publications

References 26 publications

Solid supported lipid bilayers: From biophysical studies to sensor design

Solid supported lipid bilayers: From biophysical studies to sensor design

Microfluidic array platform for simultaneous lipid bilayer membrane formation

Multiplexing Ligand−Receptor Binding Measurements by Chemically Patterning Microfluidic Channels

Contact Info

Product

Resources

About