Cell-mediated reactivity against human and Trypanosoma cruzi antigens according to clinical status in Chagas' disease patients

Titto, Ernesto de; Braun, Marta; Lázzari, Julio O.; Segura, Elsa L.

doi:10.1016/0165-2478(85)90002-1

Cited by 13 publications

(7 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Trypanosoma cruzi, the causative agent of Chagas' disease, is an important cause of heart disease in Latin America (Hontebeyrie-Joskowicz 1992). The mechanisms which are responsible for the development of pathology in Chagas' disease are not fully understood, however, there is considerable experimental evidence for an immunopathogenic component of chronic disease (DeTitto et al 1985). Over the past decade, it has become clear that cytokines are integral to the complex interactions which are required for induction and regulation of immunological effector function.…”

Section: Introductionmentioning

confidence: 99%

Local and systemic cytokine expression during experimental chronic Trypanosoma cruzi infection in a Cebus monkey model

Samudio¹,

Montenegro-James

Kasamatsu³

et al. 1999

Parasite Immunology

View full text Add to dashboard Cite

Cebus apella is an acceptable model for chronic chagasic cardiomyopathy (CCC), since it is possible to experimentally induce cardiac lesions after 1 year of Trypanosoma cruzi infection. The T. cruzi Y strain, shown previously to produce CCC in C. apella monkeys, was used to experimentally infect 10 monkeys. Parasitological, serological and clinical parameters were monitored during a 19-month follow-up, and systemic cytokine responses were assessed sequentially in five monkeys selected according to the differential parasitemia pattern exhibited. Ten additional monkeys, infected with the same strain for 5, 10 and 12 years, were analysed cross-sectionally. Three monkeys/time point and one uninfected control animal were sacrificed for gross pathology, histology, presence of parasites, and local cytokine gene expression. Elevated expression of interleukin (IL)-4 was observed throughout the study in monkeys that had persistent, high parasitemias, whereas a high level of interferon (IFN)-gamma was seen in monkeys that controlled parasitemias soon after infection. Chronically infected monkeys expressed a nonpolarized, Th0-type response. Cardiac tissue collected from a monkey that succumbed to acute infection had elevated levels of proinflammatory cytokine [IL-1beta, IL-6, tumour necrosis factor-alpha] and interstitial cell adhesion molecule (ICAM)-1, platelet-derived growth factor (PDGF)-alpha, transforming growth factor (TGF)-beta and IL-10 transcripts. Cytokine production in cardiac tissue of chronically infected monkeys was also characterized by elevated expression of ICAM-1, PDGF-alpha and TGF-beta, which correlated with the detection of T. cruzi DNA by polymerase chain reaction.

show abstract

Section: Introductionmentioning

confidence: 99%

Local and systemic cytokine expression during experimental chronic Trypanosoma cruzi infection in a Cebus monkey model

Samudio¹,

Montenegro-James

Kasamatsu³

et al. 1999

Parasite Immunology

View full text Add to dashboard Cite

show abstract

“…Most patients in the chronic phase do not show any clinical symptoms of the disease, while others may present cardiac or digestive alterations. It has been demonstrated that PBMC from chagasic patients of indeterminate or cardiac clinical forms react in vitro to parasite-derived antigens [1][2][3]. Furthermore, human anti-epimastigote antibodies purified from cardiac patients' sera have been shown to stimulate PBMC in vitro [4].…”

Section: Introductionmentioning

confidence: 99%

Cytokine mRNA Profile of Peripheral Blood Mononuclear Cells Isolated from Individuals with Trypanosoma cruzi Chronic Infection

et al. 1997

View full text Add to dashboard Cite

Characterization of immunologic activities during chronic infection with Trypanosoma cruzi is critical for understanding the dynamics of human Chagas' disease. Since cytokine production is mainly regulated by transcription and mRNA stability, quantitative RT-PCR analysis gives an accurate picture of the influences of disease on cytokine profile. Using RT-PCR, the authors analysed the levels of message expression for several cytokines in peripheral blood mononuclear cells (PBMC) freshly isolated from chagasic patients (CP) and non-infected individuals (NI), and in in vitro-stimulated PBMC from CP. Ex vivo analysis showed that mean levels of expression of IL-5, IL-10, IL-13 and IFN were dramatically increased in PBMC from CP, compared to NI. The levels of IL-2 and IL-4 were not significantly different between groups. Analysis of cytokine mRNA production after in vitro culture with parasite-derived antigens (EPI or TRP) or anti-epimastigote antibodies (Id) showed that these two classes of stimuli induced distinct cytokine responses. While EPI or TRP induced higher production of IFN specific message and low IL-10, anti-Id cells produced higher levels of IL-10 and low IFN . The simultaneous presence of antigenic and antibody stimulation in the host during the chronic phase of Chagas' disease could explain the existence of both inflammatory and anti-inflammatory cellular reactivity detected in most patients.

show abstract

“…Immunohistochemical autopsy studies of heart tissue from patients who died of cardiac disease have indicated the dominant presence of CD8 T cells in the characteristic lesions [11,12]. Furthermore, proliferation assays have indicated the involvement of T cells in parasite-specific and non-specific responses [13,14]. However, thus far the studies have dealt with whole PBMC.…”

Section: Introductionmentioning

confidence: 99%

Chagasic Patients Lack CD28 Expression on Many of Their Circulating T Lymphocytes

et al. 1996

View full text Add to dashboard Cite

A balanced host-parasite interaction during Trypanosoma cruzi infection allows for the establishment of a chronic infection that can last for many years. T cells are a major element responsible for parasite specific and non-specific immunity during the complex immune response of the host. However, the subpopulations of T cells involved in the response, as well as the exact mechanisms through which those cells are activated or rendered unresponsive, are not well defined. It is known that co-stimulatory signals, some of which are mediated via CD28, are of critical importance in the triggering of appropriate T cell responses. In this study the authors performed double-labelling studies to determine the frequency of expression of CD28 by CD4+ and CD8+ T lymphocytes in the peripheral blood of patients with Chagas' disease. The results show that chagasic patients throughout the spectrum of chronic clinical forms of the infection have significantly higher mean frequencies of CD4+CD28- and CD8+CD28-T cells, as compared with non-chagasic individuals. Considering the importance of CD28 for T-cell activation, the observed down-regulation or loss of CD28 during infection may indicate a possible basis for observed immunoregulatory events or distinct stages of T-cell activation in this infection. Recent evidence from patients with HIV/AIDS indicates that CD28- cell populations are more likely to undergo apoptosis, and increased apoptosis has been observed in experimental Chagas disease.

show abstract

Cell-mediated reactivity against human and Trypanosoma cruzi antigens according to clinical status in Chagas' disease patients

Cited by 13 publications

References 22 publications

Local and systemic cytokine expression during experimental chronic Trypanosoma cruzi infection in a Cebus monkey model

Local and systemic cytokine expression during experimental chronic Trypanosoma cruzi infection in a Cebus monkey model

Cytokine mRNA Profile of Peripheral Blood Mononuclear Cells Isolated from Individuals with Trypanosoma cruzi Chronic Infection

Chagasic Patients Lack CD28 Expression on Many of Their Circulating T Lymphocytes

Contact Info

Product

Resources

About