Cell Mediated Lympholysis in Man. The Impact of HLA‐C Antigens

Abstract

From a population of individuals, all HLA‐A, B, and C tissue typed in relation to the Sixth International Histocompatibility Workshop, an experimental investigation has been performed to study the influence of the HLA‐Cw1, w2, w3, w4, and w5 antigens in the Cell Mediated Lympholysis (CML) test. The average cytolysis obtained due to allogenic attack of one HLA‐C antigen equals 12.6%, but like the HLA‐A and B antigens, HLA‐C antigens exhibit differences with regard to sensitizing and target potential. This indicates either a heterogeneity of these antigens or the existence of separate CML determinants. It is concluded that the HLA‐C antigens may account for the cytolysis observed in some of the combinations exhibiting cytolysis which cannot be explained by the HLA‐A and B antigens.

Histocompatibility typing by cytotoxic lymphocytes. The HLA‐A2–28, B5‐w35 serologically cross‐reactive groups reviewed by cytotoxic lymphocytes

“…In this context, it must be stressed, that the CTLs investigated were raised between donors not fully matched at the HLA-C and HLA-D/DR loci. Determinants from these loci have been shown to serve as target deterrninants for CTLs (Kristensen et al 1975, Grunnet et al 1976, Feighery & Statsny 1979, Albrechtsen et al 1979, Johnsen 1980) although HLA-C determinants were found to be relatively weak targets for destruction and the influence of HLA-D/DR determinants has until now only described on non-PHAlymhoblast targets (monocytes, EBV-transformed B-cell lines and PWM transformed B-cells), even if some PHA-blasts expressed HLA-D/DR. Consequently it is possible that the immuno-dominant traits postulated here are epitopes on the HLA-C and/or HLA-D/DR molecules.…”

Human Histocompatibility Testing by T Cell‐Mediated Lympholysis: A European Standard CML Technique

“…Both products of the HLA-A, B, C (e.g. Grunnet et al 1975Grunnet et al , 1976 and the D/DR loci (Albrechtsen et al 1979, Feighery & Statsny 1979, Johnsen 1980 may be recognized by cytotoxic lymphocytes, but it is not yet clear whether the actual determinants recognized by cytotoxic T lymphocytes are identical t o those recognized by antibody, as had been assumed from early CMLstudies (reviewed by Eijsvoogel e t al. 1973).…”