Cell-mediated immunity in patients with amebic abscess of the liver

Ortiz‐Ortiz, Librado; Zamacona, G; Sepúlveda, B; Capin, N R

doi:10.1016/0090-1229(75)90046-x

Cited by 63 publications

(25 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, Velásquez and others, 55 observed proliferation of peripheral blood mononuclear cells of a patient with amoebic hepatic abscess against a 170 kDa recombinant protein. In our case we used a total extract of E. histolytica, and Talamás-Rohana and others, 28 have reported that whole extract might contain growth inhibiting factors, explaining in part the results obtained by other authors, 34,48,49 as well as the low levels of blast transformation found in all the clinical variants tested in this study, including the CG.…”

Section: Discussionmentioning

confidence: 58%

See 1 more Smart Citation

Differentiation of entamoeba histolytica/entamoeba dispar by PCR and their correlation with humoral and cellular immunity in individuals with clinical variants of amoebiasis.

Sánchez-Guillén¹,

Pérez-Fuentes

Salgado-Rosas

et al. 2002

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. To correlate a particular state of immunity with Entamoeba spp., we used colorimetric PCR to differentiate E. histolytica from E. dispar in individuals with amoebiasis and to associate its presence with the clinical profile, including humoral and cellular immune responses to E. histolytica. Our results showed high levels of antibody in acute amoebiasis and elevation of IL-4 production, a cytokine related to Th2 profile, associated with E. histolytica. In chronic amoebiasis, even with anti-E. histolytica seropositivity, intestinal symptoms were associated with E. dispar in all the cases, without differences in level of antibodies, BTI, CD4+/CD8+ ratio, INF-␥, and IL-4. Among asymptomatic carriers, E. dispar was more frequently found; however, identification of E. histolytica in two asymptomatic carriers associated with high levels of INF-␥, a cytokine related to Th1 profile, demonstrate the importance of making specific diagnosis of Entamoeba spp., to establish the clinical and epidemiological behavior in both intestinal and extra-intestinal amoebiasis.

show abstract

Section: Discussionmentioning

confidence: 58%

“…Regarding cell-mediated immunity the work of Savant, 34 Ortiz, 48 and later Salata 49 observed a poor lymphocyte response measured by BTI in circulating T lymphocytes. On the other hand, Ganguly and others, 50 demonstrated a decrease in the number of T lymphocytes circulating in the blood of patients with amoebic hepatic abscess.…”

Section: Discussionmentioning

confidence: 99%

Differentiation of entamoeba histolytica/entamoeba dispar by PCR and their correlation with humoral and cellular immunity in individuals with clinical variants of amoebiasis.

Sánchez-Guillén¹,

Pérez-Fuentes

Salgado-Rosas

et al. 2002

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

show abstract

“…Calderon et al (7) and Aust-Kettis et al (8) have shown that amebae in vitro can aggregate, ingest, and shed attached specific antiamebic antibody while remaining viable, a process that may be protective for the parasite. Normal and immune sera have been shown to be amebicidal via the alternate and classical pathways of complement (9)(10)(11); however, Reed et al (12) reported that amebae isolated from patients with liver abscess were resistant to complementmediated lysis. E. histotyica trophozoites have been characterized to be potent effector cells.…”

mentioning

confidence: 99%

Interaction of human leukocytes and Entamoeba histolytica. Killing of virulent amebae by the activated macrophage.

1985

View full text Add to dashboard Cite

IntroductionCapable effector mechanisms in the human immune response against the cytolytic, protozoan parasite Entamoeba histolytica have not been described. To identify a competent human effector cell, we studied the in vitro interactions of normal human polymorphonuclear neutrophils, peripheral blood mononuclear cells (PBMC), monocytes (MC), and MC-derived macrophages with virulent axenic amebae (strain HMI-IMSS). Amebae killed neutrophils, PBMC, MC, and MC-derived macrophages (P < 0.001), without loss of parasite viability. The addition of heat-inactivated immune serum did not enable leukocytes to kill amebae, nor did it protect these host cells from amebae. MC-derived macrophages, activated with lymphokine elicited by the mitogens conconavalin A, phytohemagglutinin, or an amebic soluble protein preparation (strain HK9), killed 55% of amebae by 3 h in a trypan blue exclusion assay (P < 0.001) during this time, 40% of the activated macrophages died. Lysis of amebae was confirmed using "'Indium oxine radiolabeled parasites and was antibody independent. Macrophage death appeared to be due to the deleterious effect of lysed amebae rather than the contact-dependent effector mechanisms of E. histolytica. Adherence between activated macrophages and amebae was greater than that between other leukocytes and amebae (P < 0.001). Microscopic observations, kinetic analysis of the killing of amebae by activated macrophages, and suspension of amebae with adherent activated macrophages in a 10% dextran solution indicated that contact by activated macrophages was necessary to initiate the killing of amebae. Catalase but not superoxide dismutase inhibited the amebicidal capacity of activated macrophages (P < 0.001). However, activated macrophages from an individual with chronic granulomatous disease were able to kill amebae, but not as effectively as normal cells (P < 0.01).In summary, activated MC-derived macrophages killed virulent E histolytica trophozoites through a contact-dependent, antibody-independent mechanism involving oxidative-dependent and -independent processes.

show abstract

“…60 Estudios sobre amibiasis experimental, efectuados en modelos animales, mostraron una baja respuesta en pruebas de hipersensibilidad tardía, aun en aquellos que presentaron altos títulos de anticuerpos. 61 Adicionalmente, al estimular la producción de MIF en los macrófagos se aborta el estado de inmunosupresión, lo cual sugiere que la respuesta podría ser modulada por el parásito o por mecanismos intrínsecos al huésped; además, linfocitos de sangre periférica, células de bazo y macrófagos peritoneales de hámsteres inmunizados, poseen actividad amebicida in vitro, 60,[62][63][64] mientras que in vivo son incapaces de eliminar al trofozoito. La presencia de ciertos factores en el suero de pacientes con amibiasis hepática puede suprimir la actividad de macrófagos de individuos normales contra el parásito in vitro, 65 la naturaleza de estos "factores" no ha sido descrita a la fecha.…”

Section: No3 Mayo-junio De 2002unclassified

Importancia de las prostaglandinas en la amibiasis hepática

Sánchez‐Ramírez

Talamás‐Rohana

2002

Salud pública Méx

View full text Add to dashboard Cite

Cell-mediated immunity in patients with amebic abscess of the liver

Cited by 63 publications

References 11 publications

Differentiation of entamoeba histolytica/entamoeba dispar by PCR and their correlation with humoral and cellular immunity in individuals with clinical variants of amoebiasis.

Differentiation of entamoeba histolytica/entamoeba dispar by PCR and their correlation with humoral and cellular immunity in individuals with clinical variants of amoebiasis.

Interaction of human leukocytes and Entamoeba histolytica. Killing of virulent amebae by the activated macrophage.

Importancia de las prostaglandinas en la amibiasis hepática

Contact Info

Product

Resources

About