Cell-mediated immune response of marmosets to herpesvirus-associated antigens in vitro

Abstract

Summary. Immune responses in vitro of some species of marmosets to herpesvirus-associated antigens expressed on virus-transformed lymphoblastoid cell lines (LCL) were studied by determining lymphocyte proliferation, interferon production, and the induction of cytotoxic effector cells in mixed lymphocyte-LCL cultures (MLLC). Autologous Epstein-Barr virus (EBV)-transformed B-cell lines induced neither lymphocyte proliferation nor interferon production in MLLC, while autologous Herpesvirus ateles (HVA)-transformed T-cell lines stimulated responder

Differential regulation of human RecQ family helicases in cell transformation and cell cycle

“…Resting B cells puri®ed from PBLs are in G 0 of the cell cycle, and they are reported by Spender et al (1999) to begin to express an early marker of cell cycle entry, cyclin D2, within 24 h after treatment with PMA or EBV. Given these facts, our results indicate (1) a sharp increase in WRN and RecQL1 occurs by PMA stimulation accompanied by clump formation of the B cells, which precedes PMAinduced cell proliferation (Abb et al, 1979;Clevers et al, 1985) and (2) up-regulation of BLM and RTS occurs when B cells initiate entry into the cell division cycle. This apparent correlation of the increase in the levels of BLM and RTS with entry into S phase of B cells agrees with the fact that TIG-3 and tumor cells in Oncogene Expression of RecQ helicases in transformed cells T Kawabe et al log phase expressed higher levels of BLM and RTS than cells in a stationary phase as will be shown later.…”

Herpesvirus ateles and herpesvirus saimiri transform marmoset T cells into continuously proliferating cell lines that can mediate natural killer cell-like cytotoxicity.

“…This finding was expected because these lines have been shown to produce small amounts of interferon (21).…”

“…Marmoset NK cells, on the other hand, seem to be much less reactive' against EBV-carrying lines (Table 3). This circumstance, together with the fact that marmoset lymphocyte cultures respond poorly against-autologous'EBV-transformed lymphoblastoid B cell lines (21), may partly explain the susceptibility of cotton-topped marmosets to tumor induction by EBV (34 Nylon wool column-passed normal human, white-lipped (WL) and cotton-topped (CT) marmoset lymphocytes, and the effector cell line 77-DI-2 were tested against four different target cells in a 6-hr 51Cr release assay. The origin and characteristics of target cells are given in Table 3.…”

“…to cause detachment of xenogeneic adherent target cells in a long-term assay (20,21 (22) and passage through nylon wool as described (23 found that all cell lines tested had the capacity to kill Molt4 cells irrespective of whether they were transformed by HVA or by HVS, tumor-derived or transformed in vitro, or newly established or old lines (Fig. 1, experiment 1).…”

Epstein‐barr virus (EBV)‐induced lymphoproliferative disease in cotton‐topped marmosets

“…It would thus appear that the EBV-induced "lymphomas" in these marmosets and perhaps in previously described lesions in the cotton-topped marmoset are more akin to fatal infectious mononucleosis and the EBV-associated proliferation in renal transplant or XLP patients. This susceptibility to EBV-provoked disease may be explained by an immunologic deficiency expressed by marmosets, especially to EBV, related to their hematopoietic chimerism (Nickerson and Gengozian, 1981) and/or defective in vitro immunological reactivity to herpesvirus-transformed cell lines (Abb et al, 1980). The similarities between these diseases observed in the marmoset and EBV infectious in man indicate that these animals will be a useful model for studying the immunopathology of EBV-induced lymphoproliferative diseases of humans.…”