Cell mediated cytotoxicity to human melanoma cell lines: Nonspecific active in vitro augmentation

Coates, Alan S.; Crawford, M

doi:10.1007/bf00200071

Cited by 1 publication

(1 citation statement)

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“…EAE was induced in inbred female 7to 10-week-old Lewis rats (C.S.E.A.L., CNRS, OrlCans la Source, France) by injecting in one hind footpad 100 p1 containing 100 pg of MBP emulsified in CFA (Difco, Detroit, MI) with 2.5 x lo9 killed Bordetellu pertussis (B. pertussis) organisms (Institut Pasteur, Paris, France) Virtually every challenged rat developed severe paralysis, fecal impaction and urinary dysfunction, on days 11 to 15 with recovery from the disease on days 16 to 17; such exceptions as there were died on days 13 to 14. Rats were scored daily for typical signs of EAE following Coates [9]: no EAE = 0; flaccid tail = 1; hind leg weakness = 2; hind leg paralysis with incontinence = 3; moribund state = 4. Two preparations of MBP were used for all the experiments.…”

Section: Eae Inductionmentioning

confidence: 99%

Suppressor cells in Lewis rats with experimental allergic encephalomyelitis: Prevention of the disease and inhibition of lymphocyte proliferation by the suppressor cells or their products

Béraud¹,

Varriale²,

Farnarier³

et al. 1982

Eur J Immunol

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Lewis rats primed with myelin basic protein (MBP) in complete Freund's adjuvant develop experimental allergic encephalomyelitis (EAE) and suddenly recover 15 to 17 days later. It was previously found that nondraining lymph node (non-DLN) cells taken at the time of convalescence and transferred into syngeneic normal animals inhibit the subsequent induction of EAE. In this report, it is shown that a suppressive factor can be extracted from non-DLN cells which mimics the inhibitory effect of cells when injected into the recipients. Non-DLN cells keep their suppressive activity on the induction of EAE after a culture of 48 h but their supernatant of culture failed to exert any protective effect in vivo. However, in vitro both the culture supernatant and the suppressor cells were found to have an inhibitory effect on the proliferative response of immune lymphoid cells to the antigen (MBP).

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Section: Eae Inductionmentioning

confidence: 99%