Cell-mediated and humoral immunity and phagocytic ability in chicken Lines divergently selected for serum immunoglobulin M and G levels

Abstract

Humoral and cell-mediated immunities and phagocytic ability were examined at the third and fourth generations of selection in two pairs of chicken lines (at 10 wk of age) that were divergently selected for levels of high serum Ig M (HIM), low serum Ig M (LIM), high serum Ig G (HIG), and low serum Ig G (LIG). Cell-mediated immunity was examined by splenomegaly assay at 12 wk of age. At 20 and 23 wk of age, 20 birds from the respective lines were injected Brucella abortus (BA), and blood samples were collected at 7 and 14 d postprimary immunization (PPI) and postsecondary immunization (PSI). Phagocytic ability was measured by carbon clearance assay at 25 and 30 wk of age. The results showed that the LIG line had higher degree of splenomegaly indices than the HIG line in both generations. The HIM and HIG lines had significantly (P < 0.05) higher total antibody titers to BA than their low counterparts. Similarly, mercaptoethanol-resistant (MER) antibody titers to BA, as measured only in the fourth generation, were significantly (P < 0.05) higher in the HIM and HIG lines than their low counterparts. In both generations, the HIM and HIG lines had significantly (P < 0.01) faster carbon clearance ability than the LIM and LIG lines. The results suggest that both pairs of selected lines exhibited divergence in immunocompetence, although they had been selected for serum Ig isotypes.

Influence of breed, heterozygosity, and disease incidence on estimates of variance components of respiratory disease in preweaned beef calves

“…If this assumption is correct, then selection for BRD resistance or tolerance may gradually result in an improved generalized disease defense mechanism, possibly a more efficacious immune system. Previous studies have demonstrated that antibody and/or cell-mediated immune responses could be used to improve health and production in cattle (Herná ndez et al, 2003), swine (Mallard et al, 1992(Mallard et al, , 1998, and poultry (Sarke et al, 2000). Further investigations into the genetic mechanisms of disease resistance are certainly warranted.…”

Evaluation of immune responses of cattle as a means to identify high or low responders and use of a human microarray to differentiate gene expression

“…Studies of mice have shown that selection for H or L antibody to sheep red blood cells is inversely associated with CMIR, and that the H antibody is associated with control of extracellular pathogens, but not intracellular organisms [13]. Studies of chickens also selected for the H or L antibody have reported similar findings [6,16]. However, selection of pigs for both H AMIR and CMIR indicates health and production benefits [8].…”

Effects of Different Source of Dietary Non-Starch Polysaccharides on Growth Performance, Physiological Characteristics and Gene Expression of GLUT2 in Chickens

“…Immunoglobulin M specialized for protect against antigens through the intracellular fluids. IgM is found mainly in the blood and lymph fluid, is the first antibody to be made by the body to fight a new infection [16,34]. Effect of different cereals on immune system depends on several factors such as genetic and variety of plant, growth condition and levels of nutrients as well as anti-nutrients in plant which well affected immune system and production of antibodies through the immune organelles such as bursa of fabricius and thymus [15].…”