Cell-matrix mechanical interaction in electrospun polymeric scaffolds for tissue engineering: Implications for scaffold design and performance

Kennedy, Kelsey M.; Bhaw‐Luximon, Archana; Jhurry, Dhanjay

doi:10.1016/j.actbio.2016.12.034

Cited by 155 publications

(112 citation statements)

References 151 publications

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“…Importantly, the coexistence of different pore sizes within the same scaffold may benefit its performance, as it has been suggested that such dispersion may be needed to promote a combination of tissue regenerative behaviors, such as infiltration and ECM secretion. [38] Concretely, it has been reported that pore sizes smaller than 12.5 µm can promote ECM production, while pores bigger than 20 µm are required for cell infiltration. [39] The pore size distribution has its basis on the experimental conditions used, in which a monodisperse population of salt particles was not sieved, but a polydisperse one.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Macroporous click-elastin-like hydrogels for tissue engineering applications

Fernández‐Colino

Wolf

Keijdener

et al. 2018

Materials Science and Engineering: C

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Elastin is a key extracellular matrix (ECM) protein that imparts functional elasticity to tissues and therefore an attractive candidate for bioengineering materials. Genetically engineered elastin-like recombinamers (ELRs) maintain inherent properties of the natural elastin (e.g. elastic behavior, bioactivity, low thrombogenicity, inverse temperature transition) while featuring precisely controlled composition, the possibility for biofunctionalization and non-animal origin. Recently the chemical modification of ELRs to enable their crosslinking via a catalyst-free click chemistry reaction, has further widened their applicability for tissue engineering. Despite these outstanding properties, the generation of macroporous click-ELR scaffolds with controlled, interconnected porosity has remained elusive so far. This significantly limits the potential of these materials as the porosity has a crucial role on cell infiltration, proliferation and ECM formation. In this study we propose a strategy to overcome this issue by adapting the salt leaching/gas foaming technique to click-ELRs. As result, macroporous hydrogels with tuned pore size and mechanical properties in the range of many native tissues were reproducibly obtained as demonstrated by rheological measurements and quantitative analysis of fluorescence, scanning electron and two-photon microscopy images. Additionally, the appropriate size and interconnectivity of the pores enabled smooth muscle cells to migrate into the click-ELR scaffolds and deposit extracellular matrix. The macroporous structure together with the elastic performance and bioactive character of ELRs, the specificity and non-toxic character of the catalyst-free click-chemistry reaction, make these scaffolds promising candidates for applications in tissue regeneration. This work expands the potential use of ELRs and click chemistry systems in general in different biomedical fields.

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Section: Accepted Manuscriptmentioning

confidence: 99%

Macroporous click-elastin-like hydrogels for tissue engineering applications

Fernández‐Colino

Wolf

Keijdener

et al. 2018

Materials Science and Engineering: C

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“…In regenerative medicine to establish a successful tissue formation, crucial is the integration of cells with the scaffold . It can be driven by materials surface properties, such as electric potential, chemical composition, wettability, and roughness, therefore surface geometry . The ideal scaffold provides mechanical support for cells attachment and creates the 3D microenvironment reassembling native extracellular matrix (ECM).…”

Section: Introductionmentioning

confidence: 99%

Single‐Step Approach to Tailor Surface Chemistry and Potential on Electrospun PCL Fibers for Tissue Engineering Application

Metwally

Karbowniczek

Szewczyk

et al. 2018

Adv Materials Inter

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A single‐step electrospinning approach enables controlling surface potential of fibers by changing voltage polarities during scaffolds production to enhance cells biointegration. This innovative and facile way of fibers production regulates the interfacial properties to enhance cells adhesion and filopodia formation on fibrous tissue scaffolds for possible bone regeneration. Tuning surface chemistry of polycaprolactone (PCL) by altering voltage polarity during electrospinning allows to double the surface potential on fibers up to 145 mV, which is directly measured using Kelvin probe force microscopy. The obtained surface potential on PCL fibers is directly correlated with surface chemistry analyzed at the grazing angle by X‐ray photoelectron spectroscopy, showing lower oxygen content at PCL fiber surfaces, produced with negative voltage polarity, PCL (−). These fibers create well‐engineered scaffolds that are able to increase significantly cell proliferation that is visualized with fluorescence microscopy, and filopodia formation on positively charged fibers, investigated with high‐resolution scanning electron microscopy. This work introduces electrospun PCL fibers without a need for chemical modification to tune electrostatic interactions between cells and fibrous scaffolds for biomaterials used in regenerative medicine.

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“…The fibers showed a mean width of 1.45 ± 0.32 µm, which is in the range reported for the native elastin fibers in the vascular tunica media (0.9–1.7 µm) and the dermis (1.0–3.5 µm) . Extensive research has been focused on unraveling how a cell responds to the different environments created by micro‐ and nano‐fibers . Some studies point to preferential adhesion of the cells to nanofibers over microfibers .…”

mentioning

confidence: 71%

Combining Catalyst‐Free Click Chemistry with Coaxial Electrospinning to Obtain Long‐Term, Water‐Stable, Bioactive Elastin‐Like Fibers for Tissue Engineering Applications

Fernández‐Colino

Wolf

Rütten

et al. 2018

Macromolecular Bioscience

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Elastic fibers are a fundamental requirement for tissue-engineered equivalents of physiologically elastic native tissues. Here, a simple one-step electrospinning approach is developed, combining i) catalyst-free click chemistry, ii) coaxial electrospinning, and iii) recombinant elastin-like polymers as a relevant class of biomaterials. Water-stable elastin-like fibers are obtained without the use of cross-linking agents, catalysts, or harmful organic solvents. The fibers can be directly exposed to an aqueous environment at physiological temperature and their morphology maintained for at least 3 months. The bioactivity of the fibers is demonstrated with human vascular cells and the potential of the process for vascular tissue engineering is shown by fabricating small-diameter tubular fibrous scaffolds. Moreover, highly porous fluffy 3D constructs are obtained without the use of specially designed collectors or sacrificial materials, further supporting their applicability in the biomedical field. Ultimately, the strategy that is developed here may be applied to other click systems, contributing to expanding their potential in medical technology.

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Cell-matrix mechanical interaction in electrospun polymeric scaffolds for tissue engineering: Implications for scaffold design and performance

Cited by 155 publications

References 151 publications

Macroporous click-elastin-like hydrogels for tissue engineering applications

Macroporous click-elastin-like hydrogels for tissue engineering applications

Single‐Step Approach to Tailor Surface Chemistry and Potential on Electrospun PCL Fibers for Tissue Engineering Application

Combining Catalyst‐Free Click Chemistry with Coaxial Electrospinning to Obtain Long‐Term, Water‐Stable, Bioactive Elastin‐Like Fibers for Tissue Engineering Applications

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