Cell–Matrix Contact Prevents Recognition and Damage of Endothelial Cells in States of Heightened Immunity

Methe, Heiko; Edelman, Elazer R.

doi:10.1161/circulationaha.105.000687

Cited by 20 publications

(18 citation statements)

References 22 publications

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“…Endothelial cells (ECs) embedded within a 3D denatured collagen matrix secrete antiproliferative, antithrombotic, and antiinflammatory agents that promote vascular repair even when such constructs are placed far from the lumen within the vascular adventitia. Luminal inflammation, occlusive thrombosis, and intimal hyperplasia can be controlled by allo-and xenogeneic matrix-embedded (ME) ECs in a manner indistinct from native endothelium and without eliciting a significant immune response (1)(2)(3)(4)(5)(6)(7)(8). The challenge posed is whether endothelial implants recapitulate the endothelium lining as an epithelium or add to the regulation by perfusing vessels of the vasa vasorum, which are principally comprised of ECs.…”

mentioning

confidence: 99%

Tissue-engineered endothelial and epithelial implants differentially and synergistically regulate airway repair

Zani

Kojima²,

Vacanti³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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The trilaminate vascular architecture provides biochemical regulation and mechanical integrity. Yet regulatory control can be regained after injury without recapitulating tertiary structure. Tissue-engineered (TE) endothelium controls repair even when placed in the perivascular space of injured vessels. It remains unclear from vascular repair studies whether endothelial implants recapitulate the vascular epithelial lining or expose injured tissues to endothelial cells (ECs) with unique healing potential because ECs line the vascular epithelium and the vasa vasorum. We examined this issue in a nonvascular tubular system, asking whether airway repair is controlled by bronchial epithelial cells (EPs) or by ECs of the perfusing bronchial vasculature. Localized bronchial denuding injury damaged epithelium, narrowed bronchial lumen, and led to mesenchymal cell hyperplasia, hypervascularity, and inflammatory cell infiltration. Peribronchial TE constructs embedded with EPs or ECs limited airway injury, although optimum repair was obtained when both cells were present in TE matrices. EC and EP expression of PGE 2, TGF␤1, TGF␤2, GM-CSF, IL-8, MCP-1, and soluble VCAM-1 and ICAM-1 was altered by matrix embedding, but expression was altered most significantly when both cells were present simultaneously. EPs may provide for functional control of organ injury and fibrous response, and ECs may provide for preservation of tissue perfusion and the epithelium in particular. Together the two cells optimize functional restoration and healing, suggesting that multiple cells of a tissue contribute to the differentiated biochemical function and repair of a tissue, but need not assume a fixed, ordered architectural relationship, as in intact tissues, to achieve these effects.tissue engineering ͉ airway disease ͉ vascular disease

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mentioning

confidence: 99%

Tissue-engineered endothelial and epithelial implants differentially and synergistically regulate airway repair

Zani

Kojima²,

Vacanti³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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“…porous matrices of denatured collagen retain the biosecretory capacity of quiescent endothelial cells and are immunoprotected. Perivascular matrix-embedded allogeneic endothelial cell implants in immunocompetent mice and rats did not induce endothelial-specific antibodies and showed a complete absence of the infiltration of innate and adaptive immune cells that normally cause rejection in engrafted tissues [29][30][31]. Perhaps surprisingly, the same was observed even in xenogeneic implants into immunocompetent hosts [32].…”

Section: The Development Of Endothelial Cell-based Paracrine Tissmentioning

confidence: 99%

“…While pro-inflammatory cytokine stimulation of 2-D cultured endothelial cells resulted in this dysfunctional phenotype, matrix-embedded endothelial cells maintained a quiescent phenotype that, in fact, induces an anti-inflammatory effect in T cells and dendritic cells-two cell types that are critical to the immune response [32]. Implanted matrix-embedded endothelial cells, as compared with 2-D cultured endothelial cells, limited naïve T cell activation, differentiation, and proliferation, and decreased the differentiation of CD4 + and CD8 + T cells into effector cells, even in animal hosts with preformed antiendothelial cell immunity [29][30][31]. Furthermore, there was enhanced induction of anti-inflammatory T reg cells [35] and T h 2 cells that are responsible not just for allograft acceptance, but also for nonfibrotic tissue repair [36,37].…”

Section: The Development Of Endothelial Cell-based Paracrine Tissmentioning

confidence: 99%

Paracrine Regulation from Tissue Engineered Constructs

Gadish

Edelman

2015

Translational Regenerative Medicine

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“…Sağ ve soldan yapılan ölçümlerin ortalaması KİMK olarak kaydedildi. (4)(5)(6). Üremik hastalarda da kardiyovasküler hastalığın ilk adımının endotel işlev bozukluğu olduğu ve bu patolojinin böbrek işlev kaybı ile paralel olarak arttığı kabul edilmektedir (7).…”

Section: Türk Nefroloji Diyaliz Ve Transplantasyon Dergisi Turkish Neunclassified

Evaluation of Carotis Intima Media Thicknesses in Varying Degrees of Renal Failure

Gürsu¹,

Aydın²,

Uzun³

et al. 2012

Turk Neph Dial Transpl

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ÖzAMAÇ: Kronik böbrek hastalığında (KBH) artmış kardiyovasküler hastalık riskinin en sık nedeni arteriyel sertleşmedir. Çalışmamızda, böbrek yetersizliğinin değişen evrelerinde arteriyel sertleşmenin bir göstergesi sayılan karotis intima media kalınlıkları (KİMK) incelenmiştir. GErEÇ ve yÖnTEMlEr:Çalışma dört grup ile yürütülmüştür: Hemodiyaliz, periton diyalizi, pre-diyaliz ve kontrol grubu. Tüm gruplarda yaş, cinsiyet, vücut kitle indeksi (VKİ), sigara kullanım öyküsü, kan basıncı değerleri; hasta gruplarında birincil böbrek hastalıkları, eşlik eden hastalıklar, diyaliz süreleri kaydedildi. Tüm gruplarda hematolojik ve biyokimyasal tetkikler dışında tek bir radyolog tarafından KİMK ölçüldü. Gruplar laboratuvar ve radyolojik bulgular açısından karşılaştırıldı.BUlGUlAr: KİMK açısından gruplar arasında yapılan karşılaştırmada gruplar arası karşılaştırmada anlamlı tek fark diyaliz öncesi ve kontrol grupları arasında bulunmuştur (p=0,029). Korelasyon analizinde KİMK'nın yaş, ürik asit ve hsCRP ile pozitif korele olduğu saptandı. Çok değişkenli analizde; KİMK düzeylerinin başlıca belirleyicisinin sadece yaş (B=0,006; beta=0,513; p=0,013) olduğu görüldü.SOnUÇ: Çalışmamızda, arteriyel sertleşmenin dolaylı bir göstergesi olan karotis intima-media kalınlığının KBH evresi ile korelasyonu gösterilememiştir. Ancak yaş ve hsCRP ile olan ilişkisi; genel popülasyonda kabul gören yaş-inflamasyon-ateroskleroz ilişkisinin üremik hastalarda da geçerli olduğunu göstermiştir.AnAhTAr SÖZcüKlEr: Ateroskleroz, Kronik böbrek hastalığı, Karotis, Doppler, Ultrasonografi ABSTrAcT OBJEcTIVE: The most frequent reason of increased cardiovascular risk in patients with chronic kidney disease (CKD) is atherosclerosis. In our study, carotid intima media thickness (CIMT), an indirect indicator of atherosclerosis, were evaluated in varying degrees of renal failure. MATErIAl and METhOdS:The study was conducted with four groups: Hemodialysis, peritoneal dialysis, pre-dialysis and control group. Age, gender, body mass index (BMI), smoking history, blood pressures of all groups; primary kidney disease, co-morbidities, dialysis duration in the patient groups were recorded. CIMT was measured in all groups by the same radiologist in addition to the hematological and biochemical tests. The groups were compared regarding laboratory and radiological findings. rESUlTS:With intergroup comparisons; the only statistically significant difference was between the predialysis and the control groups. With correlation analysis; CIMT was found to be positively correlated with age, uric acid and hsCRP levels. It was seen with multivariate analysis that the main determinant of CIMT was age (B=0.006; beta=0.513; p=0.013). cOnclUSIOn:The correlation between stage of CKD and CIMT, which is an indirect indicator of atherosclerosis, has not been shown in the present study. However, its relationship with age and hsCRP showed that the age-inflammation-atherosclerosis interrelationship accepted in the general population is also applicable to uremic patients.

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Cell–Matrix Contact Prevents Recognition and Damage of Endothelial Cells in States of Heightened Immunity

Cited by 20 publications

References 22 publications

Tissue-engineered endothelial and epithelial implants differentially and synergistically regulate airway repair

Tissue-engineered endothelial and epithelial implants differentially and synergistically regulate airway repair

Paracrine Regulation from Tissue Engineered Constructs

Evaluation of Carotis Intima Media Thicknesses in Varying Degrees of Renal Failure

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