Cell lines used for the selection of recombinant baculovirus

Abstract

Four insect cell lines were used to isolate two recombinant baculoviruses which had the beta-galactosidase (beta-gal) gene for colorimetric assay purposes. Plaque assays were performed using two Trichoplusia ni cell lines: BTI-TN-5B1-4 and TN-368, and two Spodptera frugiperda cell lines: IPLB-SF-21AE and SF9. The number of plaques (occlusion positive and blue beta-gal+ recombinants) formed in the Trichoplusia cells was higher than in the Spodoptera cells. The appearance of Autographa californica NPV polyhedra was also faster in the T. ni cell lines. The effect of cell passage on the plaque formation proved to be critical when two different passages of the SF9 cells were tested. The higher passage produced a lower viral titration. The size and time of appearance of the plaques was also different.

Optimization of chimeric HIV‐1 virus‐like particle production in a baculovirus‐insect cell expression system

“…11 In this study, two insect cell lines [S. frugiperda (fall army worm) and T. ni (cabbage looper)] were investigated as to their ability to express high yields of chimeric HIV-1 VLPs Given the more rapid doubling time of T.ni Pro TM cells, and that T.ni Pro TM cells preferentially produce higher yields of secretory recombinant proteins, 20 it was not surprising that the ANOVA data indicated these cells generally generated moderately higher yields of VLPs than Sf9 cells (Figures 4a,b,d). However, the difference in yields was not considerable, with very similar yields detected at the higher cell densities (when time post infection and MOI were kept constant) (Figure 3a) and infection times (when MOI values and cell density were kept constant) (Figure 3b).…”

Strain Identification of Spodoptera Frugiperda (Lepidoptera: Noctuidae) Insects and Cell Line: PCR-RFLP of Cytochrome Oxidase C Subunit I Gene

“…These cells are also used to study viral infection for the development of highly specific biological pesticides (Danyluk & Maruniak 1987, Sieburth & Maruniak 1988. Different clones from the same cell line can present varying susceptibility to infection by baculoviruses (Volkman & Summers 1975, Maruniak et al 1994. Cells cultured from either corn or rice strain may differentially respond to viruses, therefore, the strain identity of Sf9 could be useful information.…”