Cell lineage analysis in ascidian embryos by intracellular injection of a tracer enzyme

Nishida, Hiroki; Satoh, Noriyuki

doi:10.1016/0012-1606(83)90288-9

Cited by 290 publications

(252 citation statements)

References 12 publications

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“…The ascidian larvae exhibit the prototypical morphogenesis and body plan of chordates as characterized by the presence of a dorsal hollow neural tube, a notochord, paraxial mesoderm, and so on, while they subsequently metamorphose into adults, which are sessile animals with a unique body structure (Satoh, 1994). The ascidian tadpole larva is composed of only approximately 2,600 cells, and extensive information has been accumulated on the cell lineages for most major tissues and organs (Nishida, 1987). Due to their unique evolutionary position and their prototypical body structure, it has been proposed that analysis of ascidians would offer insights into two of the most exciting problems in evolutionary biology: the evolutionary origin of chordates from an ancestral deuterostome and the origin of vertebrates from a simple chordate (Satoh, 2003).…”

Section: Ascidiansmentioning

confidence: 99%

Organization of Hox genes in ascidians: Present, past, and future

Ikuta

Saiga

2005

Developmental Dynamics

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Section: Ascidiansmentioning

confidence: 99%

Organization of Hox genes in ascidians: Present, past, and future

Ikuta

Saiga

2005

Developmental Dynamics

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“…In ascidian larvae, the primary notochord cells are derived from the A-line blastomeres and comprise 32 cells in the anterior and middle parts of the notochord (Nishida 1987). Much less is known about the formation of the secondary notochord cells that are derived from B-line precursors and that comprise eight cells at the tip of the tail.…”

Section: Notochord Induction and Fgf Signalingmentioning

confidence: 99%

“…Muscle cells of the ascidian larva originate from three different lines of progenitor cells, the B, A and b lines (Nishida 1987). Development of the B-line (primary) muscle cells is an autonomous process.…”

Section: Formation Of Secondary Muscle Cells Requires Fgf Signalingmentioning

confidence: 99%

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Role of the FGF and MEK signaling pathway in the ascidian embryo

2001

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In the ascidian embryo, a fibroblast growth factor (FGF)-like signal from presumptive endoderm blastomeres between the 32-cell and early 64-cell stages induces the formation of notochord and mesenchyme cells. However, it has not been known whether endogenous FGF signaling is involved in the process. Here it is shown that 64-cell embryos exhibit a marked increase in endogenous extracellular signal-regulated kinase (ERK/MAPK) activity. The increase in ERK activity was reduced by treatment with an FGF receptor 1 inhibitor, SU5402, and a MEK (ERK kinase/MAPKK) inhibitor, U0126. Both drugs blocked the formation of notochord and mesenchyme when embryos were treated at the 32-cell stage, but not at the 2-or 110-cell stages. The dominant-negative form of Ras also suppressed notochord and mesenchyme formation. Both inhibitors suppressed induction by exogenous basic FGF. These results suggest that the FGF signaling cascade is indeed necessary for the formation of notochord and mesenchyme cells during ascidian embryogenesis. It is also shown that FGF signaling is required for formation of the secondary notochord, secondary muscle and neural tissues, and at least ERK activity is necessary for the formation of trunk lateral cells and posterior endoderm. Therefore, FGF and MEK signaling are required for the formation of various tissues in the ascidian embryo.

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“…The complete lineages of several invertebrates such as ascidians (Ciona intestinalis) [22][23][24], sea urchin (Strongylocentrotus purpuratus) [25,26], nematodes (Caenorhabditis elegans) [27] and leeches (Helobdella triserialis) [28] have been established using prospective clonal analysis. These methods are extensively used to study defined populations of cells in vertebrates.…”

Section: Prospective Clonal Analysismentioning

confidence: 99%

Methods in clonal analysis and applications

2005

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-During development, embryonic cells display a large variety of behaviors that lead to the formation of embryonic structures that are frequently transient. Simultaneously, cells progress towards a specific fate. The current challenge for embryologists is to resolve how these two distinct aspects of development co-exist. As cell behaviors (including elementary cellular operations such as motility, adhesiveness, polarization, change in shape, division and death) and their control are much less well understood than the genetic aspects of cell fate determination, there is currently much interest in the study of cell behaviors. This mainly consists of labeling groups of cells or, less frequently, single cells and observing their descendants. In this review, we describe a few techniques for labeling groups of cells and we discuss prospective and retrospective clonal analysis, in particular the LaacZ system, in detail. We examine the information generated by these approaches.cell lineage / laacZ / cell fate assignment / developmental patterning / change in shape / cell adhesiveness / cell motility

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Cell lineage analysis in ascidian embryos by intracellular injection of a tracer enzyme

Cited by 290 publications

References 12 publications

Organization of Hox genes in ascidians: Present, past, and future

Organization of Hox genes in ascidians: Present, past, and future

Role of the FGF and MEK signaling pathway in the ascidian embryo

Methods in clonal analysis and applications

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