Cell-line-dependent crystal morphology and sublocalization of the Thyrinteina arnobia cypovirus polyhedrin expressed from a recombinant baculovirus

Silva, Leonardo A.; Ardisson-Araújo, Daniel Mendes Pereira; Morgado, Fabrício S.; Horta, André Ballerini; Lemos, Manoel Victor Franco; Wilcken, Carlos Frederico; Ribeiro, Bergmann Morais

doi:10.1007/s00705-019-04214-z

Cited by 2 publications

(1 citation statement)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, some advances in the study of CPVs were achieved, including data on the viral protein structure, OB assembly [7], genome composition, virus diversity [8], reverse genetics, and studies on cypovirus and host interactions [9]. Although CPVs present a potential tool for biological control in agricultural crops, their use as insecticides so far has been relatively limited due to the lack of research and a restricted number of cypoviruses identified and characterized.…”

Section: Introductionmentioning

confidence: 99%

A novel cypovirus found in a betabaculovirus co-infection context contains a poxvirus immune nuclease (poxin)-related gene

Silva

Ardisson-Araújo

Camargo

et al. 2020

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

The cassava hornworm Erinnyis ello ello (Lepidoptera: Sphingidae) is an important pest in Brazil. This insect feeds on host plants of several species, especially Manihot esculenta (cassava) and Hevia brasiliensis (rubber tree). Cassava hornworm outbreaks are quite common in Brazil and can cause great impact over crop production. Granulare and polyhedral-shaped occlusion bodies (OBs) were observed in extracts of dead E. ello larvae from rubber-tree plantations by light and scanning electron microscopy (SEM), suggesting a mixed infection. The polyhedral-shaped OB surface revealed indentations that resemble those found in cypovirus polyhedra. After OB nucleic acid extraction followed by cDNA production and Illumina deep-sequencing analysis, the results confirmed for the presence of a putative novel cypovirus that carries ten segments and also a betabaculovirus (Erinnyis ello granulovirus, ErelGV). Phylogenetic analysis of the predicted segment 1-enconded RdRP showed that the new cypovirus isolate is closely related to a member of species Cypovirus 2, which was isolated from Inachis io (Lepidoptera: Nymphalidae). Therefore, we named this new isolate Erinnyis ello cypovirus 2 (ErelCPV-2). Genome in silico analyses showed that ErelCPV-2 segment 8 (S8) has a predicted amino acid identity of 35.82 % to a hypothetical protein of betabaculoviruses. This putative protein has a cGAMP-specific nuclease domain related to the poxvirus immune nucleases (poxins) from the 2′,3′-cGAMP-degrading enzyme family.

show abstract

Section: Introductionmentioning

confidence: 99%