Cell laden hydrogel construct on-a-chip for mimicry of cardiac tissue in-vitro study

pH-Sensitive Chitosan Hydrogel with Instant Gelation for Myocardial Regeneration

“…Figures 3C and 3F show the formation of large gaps and enlargement of the pores up to 500 µm because of gradual hydrogels collapse and degradation after 10 days of immersion in PBS. This is a desirable structural property of hydrogel for cell growth and proliferation with simultaneous tissue regeneration; because the cells will have more spaces for growth and receive more nutrients and oxygen through these large pores to increase their population until complete regeneration [45].…”

Hydrogels as artificial matrices for cell seeding in microfluidic devices

“…20 Studies indicate the importance of hydrogels because of their capability to form complex networks and well controlled properties. 21,22 Polymers of natural and synthetic origin are used as hydrogels in microuidic devices. 23,24 In general, natural hydrogels are typically formed from biologically derived precursors such as ECM proteins: collagen, brin, and/or hyaluronic acid, along with the polymers derived from natural sources such as chitosan and alginate.…”

“…90,91 Chitosan hydrogel has been used as a scaffold to mimic the in vitro cardiac tissue microenvironment. 21 Chitosan (1%) was used to culture cardiomyocytes on microuidic chip. Cell medium perfusion was carried out at the rate of 0.1 ml h À1 aer the attachment of the cells to the hydrogel scaffold.…”

Advanced 3D Cell Culture Techniques in Micro-Bioreactors, Part II: Systems and Applications

“…Afterwards the aggregates were encapsulated in poly(ethylene glycol) diacrylate (PEGDA) hydrogels and injected in a microfluidic, superfused PDMS-chip with C-shaped traps (Figure 20A). Ghiaseddin et al [187] used a PDMS-chip bonded onto a glass slide for perfusion of cells isolated from murine embryonal myocardial sections in chitosan hydrogels. In the microfluidic chip by Goldman et al [188] MSCs are cultivated in an agarose gel under superfusion.…”

“…The results of the simulation suggest that the ratio between Young's modulus of the cell and that of hydrogel has a strong impact on cellular deformation in a compressed matrix. However, the simulation of the flow profile as well as the shear stress was of major importance in most of the work [25,27,36,37,88,92,116,118,140,147,150,154,186,187,198,208,218,[245][246][247]. Furthermore, the oxygen distribution [17,147,150,198,218] and mass transport of metabolites [17,28,36,37,105,246,247] within the respective bioreactor system were simulated as well as the distribution of a static magnetic [123] or electric field [193].…”