Cell-laden alginate hydrogels for the treatment of diabetes

Lukin, Izeia; Eufrásio-da-Silva, Tatiane; Dolatshahi-Pirouz, Alireza; Ostrovidov, Serge; Paolone, Giovanna; Peppas, Nicholas A.; Vos, Paul de; Emerich, Dwaine F.; Orive, Gorka

doi:10.1080/17425247.2020.1778667

Cited by 10 publications

(6 citation statements)

References 32 publications

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“…Several alginate analogs have been used to enhance biocompatibility. These analogs do not trigger strong inflammatory responses and possess similar behavioral characteristics under similar conditions [ 165 ]. For example, chitosan and alginate can tolerate acidic pH and do not show toxic effects during in vitro and in vivo studies [ 166 ].…”

Section: Applications Of Alginate In the Medical Fieldmentioning

confidence: 99%

“…Consequently, management of obesity requires a comprehensive approach, among which dietary modifications and physical activity play the most important role. Observational as well as interventional studies show that the increased consumption of dietary fiber is associated with weight loss, and alternative treatments with dietary fiber have made their way up to clinical trials, with various degrees of success [ 165 ]. Given its effects in reducing inflammation and postprandial blood glucose, alginate has been considered as an agent in the treatment of obesity.…”

Section: Applications Of Alginate In the Medical Fieldmentioning

confidence: 99%

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Alginate: From Food Industry to Biomedical Applications and Management of Metabolic Disorders

et al. 2020

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Initially used extensively as an additive and ingredient in the food industry, alginate has become an important compound for a wide range of industries and applications, such as the medical, pharmaceutical and cosmetics sectors. In the food industry, alginate has been used to coat fruits and vegetables, as a microbial and viral protection product, and as a gelling, thickening, stabilizing or emulsifying agent. Its biocompatibility, biodegradability, nontoxicity and the possibility of it being used in quantum satis doses prompted scientists to explore new properties for alginate usage. Thus, the use of alginate has been expanded so as to be directed towards the pharmaceutical and biomedical industries, where studies have shown that it can be used successfully as biomaterial for wound, hydrogel, and aerogel dressings, among others. Furthermore, the ability to encapsulate natural substances has led to the possibility of using alginate as a drug coating and drug delivery agent, including the encapsulation of probiotics. This is important considering the fact that, until recently, encapsulation and coating agents used in the pharmaceutical industry were limited to the use of lactose, a potentially allergenic agent or gelatin. Obtained at a relatively low cost from marine brown algae, this hydrocolloid can also be used as a potential tool in the management of diabetes, not only as an insulin delivery agent but also due to its ability to improve insulin resistance, attenuate chronic inflammation and decrease oxidative stress. In addition, alginate has been recognized as a potential weight loss treatment, as alginate supplementation has been used as an adjunct treatment to energy restriction, to enhance satiety and improve weight loss in obese individuals. Thus, alginate holds the promise of an effective product used in the food industry as well as in the management of metabolic disorders such as diabetes and obesity. This review highlights recent research advances on the characteristics of alginate and brings to the forefront the beneficial aspects of using alginate, from the food industry to the biomedical field.

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Section: Applications Of Alginate In the Medical Fieldmentioning

confidence: 99%

Section: Applications Of Alginate In the Medical Fieldmentioning

confidence: 99%

Alginate: From Food Industry to Biomedical Applications and Management of Metabolic Disorders

et al. 2020

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“…As previously discussed, macroencapsulation devices have some limitations due to their complicated designs, low mass transport, and limited cell response ( Espona-Noguera et al, 2019 ). Furthermore, the slow diffusion of oxygen into this device may cause delays in insulin release leading to hypoglycemia ( Izeia et al, 2020 ). However, macrodevices are safe; they can reduce the risk of teratoma formation, cells’ survival, and their secreted insulin for months.…”

Section: Clinical Trials Of Islet Encapsulation and Commercialized Productsmentioning

confidence: 99%

An Overview of Engineered Hydrogel-Based Biomaterials for Improved β-Cell Survival and Insulin Secretion

Ghasemi

Akbari

Imani

2021

Front. Bioeng. Biotechnol.

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Islet transplantation provides a promising strategy in treating type 1 diabetes as an autoimmune disease, in which damaged β-cells are replaced with new islets in a minimally invasive procedure. Although islet transplantation avoids the complications associated with whole pancreas transplantations, its clinical applications maintain significant drawbacks, including long-term immunosuppression, a lack of compatible donors, and blood-mediated inflammatory responses. Biomaterial-assisted islet transplantation is an emerging technology that embeds desired cells into biomaterials, which are then directly transplanted into the patient, overcoming the aforementioned challenges. Among various biomaterials, hydrogels are the preferred biomaterial of choice in these transplants due to their ECM-like structure and tunable properties. This review aims to present a comprehensive overview of hydrogel-based biomaterials that are engineered for encapsulation of insulin-secreting cells, focusing on new hydrogel design and modification strategies to improve β-cell viability, decrease inflammatory responses, and enhance insulin secretion. We will discuss the current status of clinical studies using therapeutic bioengineering hydrogels in insulin release and prospective approaches.

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“…Due to this difficulty in the transfer of microcapsules to larger animals, and the risk of thrombosis in case of intravascular macrocapsules, extravascular macroencapsulation strategies, often based on alginate, are currently regarded as the most promising option as summarised in a recent review [ 23 ]. In general, macroencapsulation approaches have a number of advantages for the transplantation of pancreatic islets, such as the possibility for a high islet density and easy retrievability.…”

Section: Introductionmentioning

confidence: 99%

Viability and Functionality of Neonatal Porcine Islet-like Cell Clusters Bioprinted in Alginate-Based Bioinks

et al. 2022

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The transplantation of pancreatic islets can prevent severe long-term complications in diabetes mellitus type 1 patients. With respect to a shortage of donor organs, the transplantation of xenogeneic islets is highly attractive. To avoid rejection, islets can be encapsulated in immuno-protective hydrogel-macrocapsules, whereby 3D bioprinted structures with macropores allow for a high surface-to-volume ratio and reduced diffusion distances. In the present study, we applied 3D bioprinting to encapsulate the potentially clinically applicable neonatal porcine islet-like cell clusters (NICC) in alginate-methylcellulose. The material was additionally supplemented with bovine serum albumin or the human blood plasma derivatives platelet lysate and fresh frozen plasma. NICC were analysed for viability, proliferation, the presence of hormones, and the release of insulin in reaction to glucose stimulation. Bioprinted NICC are homogeneously distributed, remain morphologically intact, and show a comparable viability and proliferation to control NICC. The number of insulin-positive cells is comparable between the groups and over time. The amount of insulin release increases over time and is released in response to glucose stimulation over 4 weeks. In summary, we show the successful bioprinting of NICC and could demonstrate functionality over the long-term in vitro. Supplementation resulted in a trend for higher viability, but no additional benefit on functionality was observed.

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Cell-laden alginate hydrogels for the treatment of diabetes

Cited by 10 publications

References 32 publications

Alginate: From Food Industry to Biomedical Applications and Management of Metabolic Disorders

Alginate: From Food Industry to Biomedical Applications and Management of Metabolic Disorders

An Overview of Engineered Hydrogel-Based Biomaterials for Improved β-Cell Survival and Insulin Secretion

Viability and Functionality of Neonatal Porcine Islet-like Cell Clusters Bioprinted in Alginate-Based Bioinks

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