Cell inactivation studies on yeast cells under euoxic and hypoxic condition using electron beam from microtron accelerator

Joseph, Praveen; Acharya, Santhosh; Sanjeev, Ganesh; Bhat, NN; Narayana, Y.

doi:10.1007/s10967-011-1229-1

Cited by 8 publications

(2 citation statements)

References 15 publications

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“…The details of the Microtron are given elsewhere 11) . Fricke dosimeter was used to calibrate and optimize the absorbed dose as described elsewhere 12 - 17) . The dose rate of the gamma chamber and Microtron accelerator was determined by the same method and was found to be 1.5 Gy.min -1 and 100 Gy.min -1 respectively.…”

Section: Methodsmentioning

confidence: 99%

Standardization of CalyculinA induced PCC assay and its advantages over Okadaic acid PCC assay in Biodosimetry applications

Nairy¹,

Narayana

Bhat

et al. 2016

Journal of Occupational Health

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Objective: In the present study an attempt was made to estimate coefficients of dose response curves for PCC aberrations induced by CalyculinA and Okadaic acid, using 60Co gamma radiation and 8 MeV pulsed electron beam for biodosimetry application.Materials and Methods: The modified method outlined by Puig et al. 2013 was used to conduct Calyculin A and Okadaic acid induced PCC assay in human blood lymphocytes.Chemical treatment was given for the last 1 h of a 48 h culture. The study was carried out in the dose range 2.5 to 20 Gy using 60Co gamma rays and 8 MeV pulsed electron beam.Results and Conclusions: Results show a linear dose dependent increase with a slope of 0.047 ± 0.001 from Calycalin A PCC and 0.048 ± 0.002 form Okadaic acid PCC. The slope of the fragments curve was 0.327 ± 0.006 from Calyculin A and 0.328 ± 0.006 from Okadaic acid PCC. Further, dose calibration studies were carried out for 8 MeV electron using Calyculin A PCC assay and the obtained slope from ring yield was 0.054 ± 0.002 and 0.427 ± 0.009 from fragment yield.

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Section: Methodsmentioning

confidence: 99%

Standardization of CalyculinA induced PCC assay and its advantages over Okadaic acid PCC assay in Biodosimetry applications

Nairy¹,

Narayana

Bhat

et al. 2016

Journal of Occupational Health

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“…Cell Inactivation Studies: The survival response of Saccharomyces cerevisiae D7 strain after irradiation with proton and gamma radiation is presented in Figure 2.It is clear from Figure 3 that dose response with proton beam is linear, whereas with gamma radiation it is sigmoid. The sigmoid dose response is due to the multi-track hit processes combined with dose rate dependent molecular repair processes [9][10][11]. The linear dose-response is due to the lethal damage which leads to cell death even at lower doses.…”

Section: Resultsmentioning

confidence: 99%

Relative Biological Effectiveness Studies Using 3 MeV Proton Beam from Folded Tandem Ion Accelerator: An Experimental and Theoretical Approach

Nairy¹,

Bhat²,

Anjaria³

et al. 2020

Recent Techniques and Applications in Ionizing Radiation Research

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Proton being the easiest light ion to accelerate and achieve desired beam profile, has been pursued as a popular particulate radiation for therapy applications. In the present study, Saccharomyces cerevisiae D7 strain was used to estimate the RBE values of the 3 MeV proton beam, and an attempt was made to derive mathematical formula for calculating RBE value with respect to the dose. Dosimetry studies were carried out using Fricke dosimetry and Semiconductor Surface Barrier detector to calibrate the absorbed doses of Gamma chamber-1200 and Folded Tandem Ion Accelerator respectively. Gold standard cell survival assay and gene conversion assay were used to compare gamma and proton radiation induced cell death and genetic endpoint. Multi target single hit model was used to derive mathematical formula for RBE estimation. The results show a linear survival-dose response after proton radiation and sigmoid survival-dose response after gamma radiation treatment. The calculated RBE value from the survival and gene conversion studies was 1.60 and 3.93, respectively. The derived mathematical formula is very useful in calculating RBE value, which varies from 3.61 to 1.80 with increasing dose. The estimated RBE value from the mathematical formula is comparable with the experimental values. With the help of the present mathematical formulation, RBE value at any dose can be calculated in the exponential and sigmoidal regions of the survival curve without actually extending the experiment in that dose region, which is not possible using conventional methods.

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In vitro DNA damage characterisation studies on plasmid pBR322 after exposure to γ radiation by 60Co

Jaiswal

Misra

Shukla

et al. 2011

J Radioanal Nucl Chem

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Cell inactivation studies on yeast cells under euoxic and hypoxic condition using electron beam from microtron accelerator

Cited by 8 publications

References 15 publications

Standardization of CalyculinA induced PCC assay and its advantages over Okadaic acid PCC assay in Biodosimetry applications

Standardization of CalyculinA induced PCC assay and its advantages over Okadaic acid PCC assay in Biodosimetry applications

Relative Biological Effectiveness Studies Using 3 MeV Proton Beam from Folded Tandem Ion Accelerator: An Experimental and Theoretical Approach

In vitro DNA damage characterisation studies on plasmid pBR322 after exposure to γ radiation by 60Co

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