Cell imaging: Beyond the limits

Bourzac, Katherine

doi:10.1038/526s50a

Cited by 5 publications

(1 citation statement)

References 7 publications

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“…9 The developed techniques can all be applied with either immunohistochemical labeling or genetic transfection and are similar in many respects, but distinct in others (data generation, speed, multiplexing, resolution, and analysis) with potential of further developments and combinations. [10][11][12] The principal energy consumer in the brain is the sodium pump (Na þ ; K þ -ATPase), a membrane-bound enzyme that harnesses energy from ATP to establish a chemoelectric gradient across the plasma membrane in mammalian cells, by transporting three Na þ out of the cell and importing two K þ while hydrolyzing one ATP. 13 The established chemoelectric gradient is later used as the energy source to drive action potentials, for secondary active transport of glucose and amino acids, and for co-and countertransport of ions in neurons.…”

Section: Introductionmentioning

confidence: 99%

Sodium pump organization in dendritic spines

2016

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Advancement in fluorescence imaging with the invention of several super-resolution microscopy modalities (e.g., PALM/STORM and STED) has opened up the possibility of deciphering molecular distributions on the nanoscale. In our quest to better elucidate postsynaptic protein distribution in dendritic spines, we have applied these nanoscopy methods, where generated results could help improve our understanding of neuronal functions. In particular, we have investigated the principal energy transformer in the brain, i.e., the [Formula: see text]-ATPase (or sodium pump), an essential protein responsible for maintaining resting membrane potential and a major controller of intracellular ion homeostasis. In these investigations, we have focused on estimates of protein amount, giving assessments of how variations may depend on labeling strategies, sample analysis, and choice of nanoscopic imaging method, concluding that all can be critical factors for quantification. We present a comparison of these results and discuss the influences this may have for homeostatic sodium regulation in neurons and energy consumption.

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