Cell Hybridization by Electrofusion on Filters

Ramos, Corinne; Bonenfant, Deborah; Teissié, Justin

doi:10.1006/abio.2001.5553

Cited by 15 publications

(10 citation statements)

References 29 publications

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“…We were especially interested in the effect that the direction of the electric field might have on cell fusion. Even though we intentionally worked at suboptimal conditions in order to be able to detect expected differences with changing the direction of electric field, fusion yield obtained in our study was comparable to the fusion yield obtained by means of electrofusion by other researchers [27][28][29]. At optimal values of studied parameters, the values of fusion yields, after the subtraction of the polynucleated cells in control samples, are approaching 20%.…”

Section: Discussionsupporting

confidence: 77%

Optimization of bulk cell electrofusion in vitro for production of human–mouse heterohybridoma cells

Trontelj

Reberšek

Kandušer

et al. 2008

Bioelectrochemistry

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Section: Discussionsupporting

confidence: 77%

Optimization of bulk cell electrofusion in vitro for production of human–mouse heterohybridoma cells

Trontelj

Reberšek

Kandušer

et al. 2008

Bioelectrochemistry

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“…We are confident that with additional optimization of the electrofusion protocol, the fusion yield can be further improved22. For example, high fusion yield can be achieved by establishing high quality cell-to-cell contact, and electrofusing cells on microchip or by sucking them as a monolayer on filter with calibrated pores3050. Furthermore, by demonstrating successful fusion between B16-F1 and CHO cells, we confirmed that ns pulses are able to fuse cells of different type and with different size.…”

Section: Discussionmentioning

confidence: 98%

Cell electrofusion using nanosecond electric pulses

Rems

Ušaj

Kandušer

et al. 2013

Sci Rep

117

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Electrofusion is an efficient method for fusing cells using short-duration high-voltage electric pulses. However, electrofusion yields are very low when fusion partner cells differ considerably in their size, since the extent of electroporation (consequently membrane fusogenic state) with conventionally used microsecond pulses depends proportionally on the cell radius. We here propose a new and innovative approach to fuse cells with shorter, nanosecond (ns) pulses. Using numerical calculations we demonstrate that ns pulses can induce selective electroporation of the contact areas between cells (i.e. the target areas), regardless of the cell size. We then confirm experimentally on B16-F1 and CHO cell lines that electrofusion of cells with either equal or different size by using ns pulses is indeed feasible. Based on our results we expect that ns pulses can improve fusion yields in electrofusion of cells with different size, such as myeloma cells and B lymphocytes in hybridoma technology.

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“…The fusion index was around 10%-15% when there was no electric field due to the division in the cell population. 15 In a single-pulsed device, the fusion index increased remarkably when the field strength in the narrow section became higher during the processing. When the field intensity was increased to 1200 V / cm, the fusion index was up to 44% ͑around 30% after deducting the fraction due to cell division͒ at all three flow rates.…”

mentioning

confidence: 99%

“…The efficiency of cell fusion by our approach was comparable to the result obtained using conventional pulse generator on the same cell type and similar buffer system. 15 We also examined the viability of cells ͑both fused and nonfused cells͒ under these conditions using SYTOX green exclusion 8 ͓Fig. 3͑b͔͒.…”

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confidence: 99%

Microfluidic cell fusion under continuous direct current voltage

Wang

2006

Applied Physics Letters

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The authors report a technique which produces cell-to-cell electrofusion using a common direct current power supply on a microfluidic platform. In the authors’ method, the cells were first conjugated based on biotin-streptavidin interaction. The electrofusion was then conducted by flowing the linked cells through a simple microfluidic channel with geometric variation under continuous direct current voltage. This microfluidics-based technique offers processing at the level of single cell pairs with efficiency comparable to that of conventional electrofusion technique based on electrical pulses.

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Cell Hybridization by Electrofusion on Filters

Cited by 15 publications

References 29 publications

Optimization of bulk cell electrofusion in vitro for production of human–mouse heterohybridoma cells

Optimization of bulk cell electrofusion in vitro for production of human–mouse heterohybridoma cells

Cell electrofusion using nanosecond electric pulses

Microfluidic cell fusion under continuous direct current voltage

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