Cell guidance on peptide micropatterned silk fibroin scaffolds

Sun, Weizhen; Taylor, Caroline S.; Zhang, Yi; Gregory, David A.; Tomeh, Mhd Anas; Haycock, John W.; Smith, Patrick J.; Wang, Feng; Xia, Qingyou; Zhao, Xiubo

doi:10.1016/j.jcis.2021.06.086

Cited by 25 publications

(23 citation statements)

References 69 publications

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“…This can be due to the transformation of the filopodia of stem cell into lamellipodia and directional extension after sensing the surrounding microenvironment, promoting cell adhesion, and proliferation 64 . Other studies also found that the micropatterning could guide the alignment and growth of cells on the surface of the scaffold for tissue engineering 65,66 . One study used two polymers, biocompatible polystyrene and biodegradable poly(lactic acid), to fabricate patterned films to study the alignment, morphology, and transdifferentiation of the bone marrow‐derived rat mesenchymal stem cells (rMSCs).…”

Section: Resultsmentioning

confidence: 99%

“…64 Other studies also found that the micropatterning could guide the alignment and growth of cells on the surface of the scaffold for tissue engineering. 65,66 One study used two polymers, biocompatible polystyrene and biodegradable poly(lactic acid), to fabricate patterned films to study the alignment, morphology, and transdifferentiation of the bone marrow-derived rat mesenchymal stem cells (rMSCs). It showed that more than 80% of rMSCs were oriented in the direction of the microgrooves, whereas cells on smooth substrates were randomly oriented.…”

Section: The Effect Of the Patterned Surface On The Stem Cell Attachm...mentioning

confidence: 99%

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Comparison of the enhanced attachment and proliferation of the human mesenchymal stem cells on the biomimetic nanopatterned surfaces of zein, silk fibroin, and gelatin

2022

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Natural proteins have been reported to positively affect the attachment and proliferation of cells. For the first time, zein, a plant protein, was utilized to make patterned surface mimicking the extracellular matrix to assist the attachment and proliferation of stem cells. Zein would promote the attachment and proliferation of the stem cells more than 10 times of that of gelatin and silk fibroin, respectively, which are popular protein selections for the formation of the biomaterial scaffolds. The more the surface was covered by zein, the more the stem cell grown. It was revealed that the stem cells would grow and stretch in the direction of the patterns, and the stem cells preferred to grow in the grooves in the size of 8 μm, that was similar to the size of the stem cells, rather than the size larger or smaller than that of the cells, such as 50 and 2 μm. It was concluded that zein is a better choice than silk fibroin and gelatin with highly potential for the formation of patterned surface and structure as the biomaterial scaffolds for stem cell therapy.

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Section: Resultsmentioning

confidence: 99%

Section: The Effect Of the Patterned Surface On The Stem Cell Attachm...mentioning

confidence: 99%

Comparison of the enhanced attachment and proliferation of the human mesenchymal stem cells on the biomimetic nanopatterned surfaces of zein, silk fibroin, and gelatin

2022

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“…Neuronal cells could then be seeded on these patterned substrates to guide the cells for directional growth. 144 Such a technique allows for micropatterns to be added on a substrate, which may be hard to achieve using extrusion or light-based printing. As such, although inkjet bioprinting may not find much development using SF bioinks, there may be opportunities to develop TE scaffolds using reactive inkjet printing.…”

Section: Target Tissue Applicationsmentioning

confidence: 99%

“…In this method, Sun et al’s team demonstrated that SF solution could be functionalized to form micropatterns on a peptide-doped substrate. Neuronal cells could then be seeded on these patterned substrates to guide the cells for directional growth . Such a technique allows for micropatterns to be added on a substrate, which may be hard to achieve using extrusion or light-based printing.…”

Section: Target Tissue Applicationsmentioning

confidence: 99%

Silk Fibroin as a Bioink – A Thematic Review of Functionalization Strategies for Bioprinting Applications

Tan

Liu

Mitryashkin

et al. 2022

ACS Biomater. Sci. Eng.

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Bioprinting is an emerging tissue engineering technique that has attracted the attention of researchers around the world, for its ability to create tissue constructs that recapitulate physiological function. While the technique has been receiving hype, there are still limitations to the use of bioprinting in practical applications, much of which is due to inappropriate bioink design that is unable to recapitulate complex tissue architecture. Silk fibroin (SF) is an exciting and promising bioink candidate that has been increasingly popular in bioprinting applications because of its processability, biodegradability, and biocompatibility properties. However, due to its lack of optimum gelation properties, functionalization strategies need to be employed so that SF can be effectively used in bioprinting applications. These functionalization strategies are processing methods which allow SF to be compatible with specific bioprinting techniques. Previous literature reviews of SF as a bioink mainly focus on discussing different methods to functionalize SF as a bioink, while a comprehensive review on categorizing SF functional methods according to their potential applications is missing. This paper seeks to discuss and compartmentalize the different strategies used to functionalize SF for bioprinting and categorize the strategies for each bioprinting method (namely, inkjet, extrusion, and light-based bioprinting). By compartmentalizing the various strategies for each printing method, the paper illustrates how each strategy is better suited for a target tissue application. The paper will also discuss applications of SF bioinks in regenerating various tissue types and the challenges and future trends that SF can take in its role as a bioink material.

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“…SF can also be blended or combined with other polymers such as alginate, chitosan, and gelatin to alter the rheological properties of the bioink and significantly improve its printability, gelation rate [ 21 ], and cell growth [ 22 ], as well as enhancing its mechanical properties and reducing the degradation rate [ 23 , 24 ]. When mixed with hydroxyapatite, the SF membrane can enhance mechanical properties and promote cell proliferation and has applications in wound healing or as printable or injectable bone filling material [ 25 ]; silk fibroin and bioactive glass hybrid 3D printed composite scaffold for bone tissue engineering [ 26 ]; and silk fibroin and I 3 K peptide nanofiber scaffold 3D printed cell scaffold [ 27 ]. It can also induce silk fibroin–hyaluronic acid to generate hydrogel by ultrasound [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Silk Protein Composite Bioinks and Their 3D Scaffolds and In Vitro Characterization

Li¹,

Zhao²,

Zhang³

2022

IJMS

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This paper describes the use of silk protein, including fibroin and sericin, from an alkaline solution of Ca(OH)2 for the clean degumming of silk, which is neutralized by sulfuric acid to create calcium salt precipitation. The whole sericin (WS) can not only be recycled, but completely degummed silk fibroin (SF) is also obtained in this process. The inner layers of sericin (ILS) were also prepared from the degummed silk in boiling water by 120 °C water treatment. When the three silk proteins (SPs) were individually grafted with glycidyl methacrylate (GMA), three grafted silk proteins (G-SF, G-WS, G-ILS) were obtained. After adding I2959 (a photoinitiator), the SP bioinks were prepared with phosphate buffer (PBS) and subsequently bioprinted into various SP scaffolds with a 3D network structure. The compressive strength of the SF/ILS (20%) scaffold added to G-ILS was 45% higher than that of the SF scaffold alone. The thermal decomposition temperatures of the SF/WS (10%) and SF/ILS (20%) scaffolds, mainly composed of a β-sheet structures, were 3 °C and 2 °C higher than that of the SF scaffold alone, respectively. The swelling properties and resistance to protease hydrolysis of the SP scaffolds containing sericin were improved. The bovine insulin release rates reached 61% and 56% after 5 days. The L929 cells adhered, stretched, and proliferated well on the SP composite scaffold. Thus, the SP bioinks obtained could be used to print different types of SP composite scaffolds adapted to a variety of applications, including cells, drugs, tissues, etc. The techniques described here provide potential new applications for the recycling and utilization of sericin, which is a waste product of silk processing.

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Cell guidance on peptide micropatterned silk fibroin scaffolds

Cited by 25 publications

References 69 publications

Comparison of the enhanced attachment and proliferation of the human mesenchymal stem cells on the biomimetic nanopatterned surfaces of zein, silk fibroin, and gelatin

Comparison of the enhanced attachment and proliferation of the human mesenchymal stem cells on the biomimetic nanopatterned surfaces of zein, silk fibroin, and gelatin

Silk Fibroin as a Bioink – A Thematic Review of Functionalization Strategies for Bioprinting Applications

Silk Protein Composite Bioinks and Their 3D Scaffolds and In Vitro Characterization

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