Cell-fusion properties of Sendai virus prepared by polyethylene-glycol precipitation

Abstract

1972. Cell-fusion properties of Sendai virus prepared by polyethylene-glycol precipitation. Can. J. Microbiol. 18: 607-610. Sendai virus was concentrated from the allantoic fluid of embryonated chicken eggs by polyethyleneglycol (PEG) precipitation. A concentration of 6 4 % PEG sufficed to precipitate essentially all of the virus (measured by hemagglutination) with maximum retention of cell-fusion ability. Electron-n~icro-scopic examination of the 6% PEG pellet revealed only intact virions. Thus the method is suitable for preparing Sendai virus for cell-fusion experiments. COOK, G. J., L. A. BABIUK et J. B. HUDSON. 1972. Cell-fusion properties of Sendai virus prepared by polyethylene-glycol precipitation. Can. J. Microbiol. 18: 607-610.Le virus Sendai a ttt concentrt, a partir du liquide allantoi'dien d'oeufs ernbryonnes de poules, par precipitation en utilisant le polyethylkne glycol (PEG). Une concentration de 6-8yo de PEG a Cte suffisante pour prkipiter essentiellement tous les virus (evaluation par hemagglutination) avec conservation maxilnuln de l'habilete a se fusionner A la culture. L'examen au microscope Clectronique des particules de la suspension a 6% de PEG revkle seulement des particules virales intactes. Cette methode convient donc pour la prtparation de virus Sendai en vue d'expkriences de fusion cellulaire.

Inhibition of polyoma virus development in mouse–hamster heterokaryons

“…The cell types used in this study were mouse embryo and occasionally mouse kidney cultures, (Cook et al 1972); preparation of polyoma antiserum in mice, and its use in the measurement of viral capsid producing cells by immunofluorescence (Petursson and Weil 1968); and autoradiography of the cell monolayers . The hamster cells were usually incubated with 2.5 to 5.0 pCi /ml of 3H-methyl-thymidine (AmershamSearle 20 Ci /mmole) over a period of 24 h before infection and cell fusion, which regularly labeled over 95y0 of their nuclei.…”