Cell-free Systems: Recent Advances and Future Outlook

Koo, Joonhoi; Yang, Jiho; Park, Hyunjun

doi:10.1007/s12257-020-0013-x

Cited by 4 publications

(2 citation statements)

References 58 publications

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“…Further, the output of vitamin products in different dimensions will be increased by transforming the complex and multi-enzyme pathways required for the production of vitamins, establishing microbial flora with controllable functions and stability, and application of some advanced engineering technology, such as the cold-shock-triggered temperature control system, dynamic control of gene expression systems, different types of biosensors, cell-free systems and computer-aided design, etc. (Koo et al, 2020;Marucci et al, 2020;Sachsenhauser et al, 2020;Shen et al, 2020;Glasscock et al, 2021). Additionally, the modular and orthogonal strategies are increasingly supporting the construction of vitamin cell factories (Liu et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Microbial Cell Factories for Green Production of Vitamins

Wang

Liu

Jin

et al. 2021

Front. Bioeng. Biotechnol.

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Vitamins are a group of essential nutrients that are necessary to maintain normal metabolic activities and optimal health. There are wide applications of different vitamins in food, cosmetics, feed, medicine, and other areas. The increase in the global demand for vitamins has inspired great interest in novel production strategies. Chemical synthesis methods often require high temperatures or pressurized reactors and use non-renewable chemicals or toxic solvents that cause product safety concerns, pollution, and hazardous waste. Microbial cell factories for the production of vitamins are green and sustainable from both environmental and economic standpoints. In this review, we summarized the vitamins which can potentially be produced using microbial cell factories or are already being produced in commercial fermentation processes. They include water-soluble vitamins (vitamin B complex and vitamin C) as well as fat-soluble vitamins (vitamin A/D/E and vitamin K). Furthermore, metabolic engineering is discussed to provide a reference for the construction of microbial cell factories. We also highlight the current state and problems encountered in the fermentative production of vitamins.

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Section: Discussionmentioning

confidence: 99%

Microbial Cell Factories for Green Production of Vitamins

Wang

Liu

Jin

et al. 2021

Front. Bioeng. Biotechnol.

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“…Cell-free systems can generally be defined as platforms where biochemical reactions occur independently of living cells. Cell-free systems are divided into two types based on the method of preparation: extract-based systems and enzyme-based systems [1]. The extract-based cell-free systems were first introduced over a century ago by Eduard Buchner.…”

Section: Introductionmentioning

confidence: 99%

Biotechnology Applications of Cell-Free Expression Systems

Brookwell

Oza

Caschera

2021

Life

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Cell-free systems are a rapidly expanding platform technology with an important role in the engineering of biological systems. The key advantages that drive their broad adoption are increased efficiency, versatility, and low cost compared to in vivo systems. Traditionally, in vivo platforms have been used to synthesize novel and industrially relevant proteins and serve as a testbed for prototyping numerous biotechnologies such as genetic circuits and biosensors. Although in vivo platforms currently have many applications within biotechnology, they are hindered by time-constraining growth cycles, homeostatic considerations, and limited adaptability in production. Conversely, cell-free platforms are not hindered by constraints for supporting life and are therefore highly adaptable to a broad range of production and testing schemes. The advantages of cell-free platforms are being leveraged more commonly by the biotechnology community, and cell-free applications are expected to grow exponentially in the next decade. In this study, new and emerging applications of cell-free platforms, with a specific focus on cell-free protein synthesis (CFPS), will be examined. The current and near-future role of CFPS within metabolic engineering, prototyping, and biomanufacturing will be investigated as well as how the integration of machine learning is beneficial to these applications.

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The contribution of microorganisms to sustainable development: towards a green future through synthetic biology and systems biology

Qumsani

2024

J.Umm Al-Qura Univ. Appll. Sci.

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Microorganisms, though invisible, they play a pivotal role in influencing both the global economy and societal progress., and job market. This discussion highlights their significant impact on various sectors like food, pharmaceuticals, and chemicals. These versatile microorganisms act as efficient cell factories, producing chemicals from renewable sources and aiding in waste degradation. The historical development of microbial cell factories has relied on a trial-and-error approach, following a cyclic process of design, construction, testing, and refinement. The essay delves into the critical role of microorganisms in sustainable development, highlighting their capacity for sustainable chemical production and waste degradation. The incorporation of microbial technology presents significant opportunities for advancing the United Nations’ Sustainable Development Goals. Microorganisms contribute significantly to sustainable development by influencing the economy, creating jobs, improving food and pharmaceutical production, and advancing chemical manufacturing. Their utilization brings advantages like cleaner production methods, renewable resource utilization, and healthcare contributions. Overall, microorganisms are essential players in sustainable development, offering solutions for a more environmentally friendly and economically viable future.

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Cell-free Systems: Recent Advances and Future Outlook

Cited by 4 publications

References 58 publications

Microbial Cell Factories for Green Production of Vitamins

Microbial Cell Factories for Green Production of Vitamins

Biotechnology Applications of Cell-Free Expression Systems

The contribution of microorganisms to sustainable development: towards a green future through synthetic biology and systems biology

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