Cell-free fetal DNA in the cerebrospinal fluid of women during the peripartum period

Angert, Robert; LeShane, Erik S.; Yarnell, R.; Johnson, Kirby L.; Bianchi, Diana W.

doi:10.1016/j.ajog.2003.10.562

Cited by 32 publications

(18 citation statements)

References 18 publications

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“…Additionally, cell-free fetal DNA and RNA have been isolated from other body fluids e.g. maternal plasma [27], amniotic fluid [28], and cerebrospinal fluid [29]. Although not tested at that time, it is quite likely that these nucleic acids are associated with microvesicles which could explain their relative stability in the nuclease-rich environment of body fluids.…”

Section: Discussionmentioning

confidence: 99%

Body fluid derived exosomes as a novel template for clinical diagnostics

et al. 2011

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BackgroundExosomes are small membrane vesicles with a size of 40-100 nm that are released by different cell types from a late endosomal cellular compartment. They can be found in various body fluids including plasma, malignant ascites, urine, amniotic fluid and saliva. Exosomes contain proteins, miRNAs and mRNAs (exosome shuttle RNA, esRNA) that could serve as novel platform for diagnosis.MethodWe isolated exosomes from amniotic fluid, saliva and urine by differential centrifugation on sucrose gradients. Marker proteins were identified by Western blot and FACS analysis after adsorption of exosomes to latex beads. We extracted esRNA from exosomes, carried out RT-PCR, and analyzed amplified products by restriction length polymorphism.ResultsExosomes were positive for the marker proteins CD24, CD9, Annexin-1 and Hsp70 and displayed the correct buoyant density and orientation of antigens. In sucrose gradients the exosomal fractions contained esRNA that could be isolated with sufficient quantity for further analysis. EsRNAs were protected in exosomes from enzymatic degradation. Amniotic fluid esRNA served as template for the typing of the CD24 single nucleotide polymorphism (rs52812045). It also allowed sex determination of the fetus based on the detection of the male specific ZFY gene product.ConclusionsOur data demonstrate that exosomes from body fluids carry esRNAs which can be analyzed and offers access to the transcriptome of the host organism. The exosomal lipid bilayer protects the genetic information from degradation. As the isolation of exosomes is a minimally invasive procedure, this technique opens new possibilities for diagnostics.

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Section: Discussionmentioning

confidence: 99%

Body fluid derived exosomes as a novel template for clinical diagnostics

et al. 2011

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“…For many years we have been studying both intact cells [1], and more recently, cell-free DNA and messenger RNA sequences that are detectable in various maternal body fluids [2], such as plasma and serum [3], cerebrospinal fluid [4], and amniotic fluid [5,6]. This is translational research, which concentrates on the development of novel prenatal diagnostic tests to improve detection of fetal abnormalities or complications of pregnancy.…”

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confidence: 99%

Fetomaternal cell trafficking: a story that begins with prenatal diagnosis and may end with stem cell therapy

Bianchi

2007

Journal of Pediatric Surgery

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“…In an initial study, Angert et al [100] considered that since CSF is secreted by the choroid plexus and, therefore, is protected by the blood-CSF barrier, a mechanism similar to the blood-brain barrier. Normally, only hydrophobic and non-polar molecules of a molecular weight of <500 Da are permitted to enter and only during e.g.…”

Section: Fetal Dna From Pregnant and Post Delivery Womenmentioning

confidence: 98%

Other Body Fluids as Non-invasive Sources of Cell-Free DNA/RNA

Hui

Maron

Gahan

2014

Advances in Predictive, Preventive and Personalised Medicine

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In addition to plasma and serum as sources of nucleic acids circulating in the whole body, amniotic fluid, saliva, urine, pleural effusion, bronchial lavage, bronchial aspirates, breast milk, colostrums, tears, seminal fluid, peritoneal fluid, pleural effusion and stools are all available for minimally invasive analysis of nucleic acids. This chapter introduces the possibilities of using nucleic acids from amniotic fluid, saliva, urine, cerebrospinal fluid and bronchial lavage/aspirates in attempts to produce reliable early markers for diagnosis, prognosis and treatment monitoring using minimally invasive methodology. Moreover, the data from amniotic fluid can be used also to further the understanding of normal and abnormal fetal development in utero. In addition, the data from saliva can be employed for monitoring the progress of premature born infants.

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Cell-free fetal DNA in the cerebrospinal fluid of women during the peripartum period

Cited by 32 publications

References 18 publications

Body fluid derived exosomes as a novel template for clinical diagnostics

Body fluid derived exosomes as a novel template for clinical diagnostics

Fetomaternal cell trafficking: a story that begins with prenatal diagnosis and may end with stem cell therapy

Other Body Fluids as Non-invasive Sources of Cell-Free DNA/RNA

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