Cell-free extracts of Propionibacterium acnes stimulate cytokine production through activation of p38 MAPK and Toll-like receptor in SZ95 sebocytes

Huang, Yu‐Chun; Yang, Chao‐Hsun; Li, Tingting; Zouboulis, Christos C.; Hsu, Han-Chi

doi:10.1016/j.lfs.2015.07.028

Cited by 51 publications

(45 citation statements)

References 36 publications

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“…To ensure bacteriological safety of cell culture incubator and to avoid an excess of bacteria multiplication during the assay, bacteria in lysate forms were preferred to viable bacteria inoculum. Immune and keratinocytes/sebocytes stimulations using bacteria in lysate forms were previously widely described, and based on these publications, we were able to use lysates to perform the experiments . To create bacteria lysates, all bacterial suspensions were calibrated at a DO = 1.00 ± 0.15, heat‐treated, and entered into a series of freezing/thawing and freezing/sonication cycles to ensure efficient mechanical lysis of their Gram‐positive cell walls.…”

Section: Methodsmentioning

confidence: 99%

“…Immune and keratinocytes/sebocytes stimulations using bacteria in lysate forms were previously widely described, and based on these publications, we were able to use lysates to perform the experiments. 25,[29][30][31] To create bacteria lysates, all bacterial suspensions were calibrated at a DO = 1.00 AE 0.15, heat-treated, and entered into a series of freezing/thawing and freezing/sonication cycles to ensure efficient mechanical lysis of their Grampositive cell walls. Viability controls were systematically performed before and after the lysis process to ensure that no viable bacteria were recovered before cocultures experiments were started.…”

Section: Bacteria Lysatesmentioning

confidence: 99%

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Cutibacterium acnes phylotypes diversity loss: a trigger for skin inflammatory process

Dagnelie

Corvec²,

Saint‐Jean

et al. 2019

Acad Dermatol Venereol

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Background Acne has long been understood as a multifactorial chronic inflammatory disease of the pilosebaceous follicle, where Cutibacterium acnes (subdivided into six main phylotypes) is a crucial factor. In parallel, the loss of microbial diversity among the skin commensal communities has recently been shown as often accompanied by inflammatory skin disorders. Objective This study investigated the association of C. acnes phylotype diversity loss and the impact on Innate Immune System (IIS) activation. Methods The IIS response of skin after incubation with phylotypes IA1, II or III individually and with the combination of IA1 + II + III phylotypes, was studied in an in vitro skin explant system. The inflammatory response was monitored by immunohistochemistry and ELISA assays, targeting a selection of Innate Immune Markers (IIMs) (IL‐6, IL‐8, IL‐10, IL‐17, TGF‐β). Results IIMs were significantly upregulated in skin when being incubated with phylotype IA1 alone compared with the combination IA1 + II + III. In parallel, ELISA assays confirmed these results in supernatants for IL‐17, IL‐8 and IL‐10. Conclusion We identify the loss of C. acnes phylotype diversity as a trigger for IIS activation, leading to cutaneous inflammation. These innovative data underline the possibility to set up new approaches to treat acne. Indeed, maintaining the balance between the different phylotypes of C. acnes may be an interesting target for the development of drugs.

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Section: Methodsmentioning

confidence: 99%

Section: Bacteria Lysatesmentioning

confidence: 99%

Cutibacterium acnes phylotypes diversity loss: a trigger for skin inflammatory process

Dagnelie

Corvec²,

Saint‐Jean

et al. 2019

Acad Dermatol Venereol

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“…ICAM-1) to facilitate recruitment of professional inflammatory cells towards the skin lesions [21]. The transcription factor NF-κB is activated in acne lesions [20], and cell-free extracts of C. acnes have been shown to promote protein phosphorylation of p38MAPK in SZ95 sebocytes [4]. …”

Section: Discussionmentioning

confidence: 99%

“…Moreover, human sebocytes are immunologically activated by bacterial proteins via TLRs and CD14, which are both expressed on their cell surface [3]. Cell-free extracts of C. acnes stimulate cytokine production through activation of TLR in SZ95 sebocytes [4]. Activation of pattern recognition receptors increases the secretion of both pro- and anti-inflammatory cytokines (chemokines [IL-8], TNF-α, antimicrobial lipids, peptides) in sebaceous glands [5-7].…”

Section: Introductionmentioning

confidence: 99%

Aryl Hydrocarbon Receptor Modulates the Expression of TNF-α and IL-8 in Human Sebocytes via the MyD88-p65NF-κB/p38MAPK Signaling Pathways

et al. 2018

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Activation of Toll-like receptor (TLR)-2 and subsequent inflammatory response contribute to lesion development in acne vulgaris. A cross-talk between aryl hydrocarbon receptor (AhR), a cytosolic receptor protein that responds to environmental and physiological stress, and TLRs has recently been reported. In this study, we explored the possible role of AhR in the effects induced on cultured human SZ95 sebocytes by peptidoglycan (PGN), a classic TLR2 agonist. PGN-induced secretion of inflammatory factors TNF-α and IL-8 in human SZ95 sebocytes was suppressed after knockdown of AhR and pretreatment with the AhR antagonist CH223191. In addition, the AhR agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) enhanced TNF-α and IL-8 secretion in PGN-pretreated sebocytes. Furthermore, PGN-induced expression of myeloid differentiation factor 88 (MyD88), phospho-p38MAPK (p-p38MAPK), and p-p65NF-κB was strengthened by TCDD and repressed by CH223191. AhR inhibition by transfecting shRNA blocked the ability of PGN to stimulate phosphorylation of p38MAPK and p65NF-κB in SZ95 sebocytes. Overall, these data demonstrate that AhR is able to modulate PGN-induced expression of TNF-α and IL-8 in human SZ95 sebocytes involving the MyD88-p65NF-κB/p38MAPK signaling pathway, which probably indicates a new mechanism in TLR2-mediated acne.

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“…Here we observed that both compounds markedly promoted HaCaT migration and enhanced artificial wound closure compromised by LPS treatment, an effect related to their ability to sustain VEGF production. Cell-free extracts of P. acnes significantly stimulate secretion of interleukins (IL-8 and IL-6) in SZ95 sebocytes [30] and promote IL-8 secretion by interacting with Toll-like receptor 2 [31]. IL-6 is a pleiotropic cytokine that plays a pivotal role in host defense, immune response regulation, hematopoiesis, and inflammation [32].…”

Section: Discussionmentioning

confidence: 99%

Pleiotropic Effects of White Willow Bark and 1,2-Decanediol on Human Adult Keratinocytes

Bassino

Gasparri

Munaron

2017

Skin Pharmacol Physiol

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Background: Acne vulgaris is a common skin defect, usually occurring during adolescence, but often it can persist in adults leaving permanent face scarring. Acne is usually treated with topical drugs, oral antibiotics, retinoids, and hormonal therapies, but medicinal plants are increasingly employed. Objective: To investigate the protective role of white willow bark (WWB) and 1,2-decanediol (DD) on the damage caused by lipopolysaccharides (LPS) on human adult keratinocytes (HaCaT). Methods: HaCaT were exposed to LPS alone or in association with WWB and DD. Epidermal viability, metabolic modulation, inflammatory activity, and cell migration were assessed with both common standardized protocols or high-throughput screening systems. Results: The preincubation of HaCaT with WWB and DD (used separately or in combination) differently prevented the alterations induced by LPS on HaCaT in terms of growth factor release (IGF, EGF, VEGF), cytokine production (IL-1α, IL-6, IL-8), or expression of the transcription factor FOXO-I. Moreover, they partially restore wound repair lowered by LPS. Conclusions: These results suggest that both natural compounds were able to differently affect several functions of LPS-stressed keratinocytes suggesting their potential role for the prevention of acne vulgaris, without adverse effects.

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Cell-free extracts of Propionibacterium acnes stimulate cytokine production through activation of p38 MAPK and Toll-like receptor in SZ95 sebocytes

Cited by 51 publications

References 36 publications

Cutibacterium acnes phylotypes diversity loss: a trigger for skin inflammatory process

Cutibacterium acnes phylotypes diversity loss: a trigger for skin inflammatory process

Aryl Hydrocarbon Receptor Modulates the Expression of TNF-α and IL-8 in Human Sebocytes via the MyD88-p65NF-κB/p38MAPK Signaling Pathways

Pleiotropic Effects of White Willow Bark and 1,2-Decanediol on Human Adult Keratinocytes

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