Cell‑free DNA as a liquid biopsy for early detection of gastric cancer (Review)

Huang, Zhengbin; Zhang, Haitao; Yu, Benjamin; Yu, Dehua

doi:10.3892/ol.2020.12264

Cited by 23 publications

(28 citation statements)

References 114 publications

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“…In the last years, two studies [15,16] identified that cfDNA was able to distinguish GC patients from healthy individuals with an area under the curve (AUC) of 0.94 and 0.99, respectively. Nevertheless, the use of cfDNA for early GC detection is difficult to implement due to its low abundance in the early stage and the technical challenges in its detection [17]. Another approach was evaluated by Cohen et al, where the authors designed the CancerSEEK blood test.…”

Section: Circulating Free Dnamentioning

confidence: 99%

“…However, the use of some of these biomarkers still has limitations. Thus, the use of cfDNA for early GC detection is difficult to implement due to the low abundance in early-stage GC and the technical challenges in its detection [17]. Furthermore, cfDNA is derived from cells that undergo necrosis or apoptosis, which might not reflect viable tumor cells [60].…”

Section: Proteinsmentioning

confidence: 99%

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Biomarkers for Gastric Cancer Screening and Early Diagnosis

et al. 2021

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Gastric cancer is one of the most common cancers worldwide, with a bad prognosis associated with late-stage diagnosis, significantly decreasing the overall survival. This highlights the importance of early detection to improve the clinical course of these patients. Although screening programs, based on endoscopic or radiologic approaches, have been useful in countries with high incidence, they are not cost-effective in low-incidence populations as a massive screening strategy. Additionally, current biomarkers used in daily routine are not specific and sensitive enough, and most of them are obtained invasively. Thus, it is imperative to discover new noninvasive biomarkers able to diagnose early-stage gastric cancer. In this context, liquid biopsy is a promising strategy. In this review, we briefly discuss some of the potential biomarkers for gastric cancer screening and diagnosis identified in blood, saliva, urine, stool, and gastric juice.

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Section: Circulating Free Dnamentioning

confidence: 99%

Section: Proteinsmentioning

confidence: 99%

Biomarkers for Gastric Cancer Screening and Early Diagnosis

et al. 2021

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“…This assay allows selection of further cancer therapy [ 12 ]. Liquid biopsy is another method of diagnosis applied especially for leukemia and gastric cancer [ 13 , 14 ]. Advanced methods of cancer diagnosis include detection of circulating cancer cells (CRC) as well as next generation sequencing (NGS) that allows for genetic characterization of malignancies [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Polymer Nanoparticles and Nanomotors Modified by DNA/RNA Aptamers and Antibodies in Targeted Therapy of Cancer

2021

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Polymer nanoparticles and nano/micromotors are novel nanostructures that are of increased interest especially in the diagnosis and therapy of cancer. These structures are modified by antibodies or nucleic acid aptamers and can recognize the cancer markers at the membrane of the cancer cells or in the intracellular side. They can serve as a cargo for targeted transport of drugs or nucleic acids in chemo- immuno- or gene therapy. The various mechanisms, such as enzyme, ultrasound, magnetic, electrical, or light, served as a driving force for nano/micromotors, allowing their transport into the cells. This review is focused on the recent achievements in the development of polymer nanoparticles and nano/micromotors modified by antibodies and nucleic acid aptamers. The methods of preparation of polymer nanoparticles, their structure and properties are provided together with those for synthesis and the application of nano/micromotors. The various mechanisms of the driving of nano/micromotors such as chemical, light, ultrasound, electric and magnetic fields are explained. The targeting drug delivery is based on the modification of nanostructures by receptors such as nucleic acid aptamers and antibodies. Special focus is therefore on the method of selection aptamers for recognition cancer markers as well as on the comparison of the properties of nucleic acid aptamers and antibodies. The methods of immobilization of aptamers at the nanoparticles and nano/micromotors are provided. Examples of applications of polymer nanoparticles and nano/micromotors in targeted delivery and in controlled drug release are presented. The future perspectives of biomimetic nanostructures in personalized nanomedicine are also discussed.

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“…Circulating free DNAs (cfDNA) are free DNA fragments found in plasma, serum, or other body fluids. Physiological events, such as apoptosis or micrometastasis are the origin of cfDNAs in the body [1]. Previous studies showed that cfDNA level is 2-3-fold higher in cancer patients compared with healthy individuals [2].…”

Section: Introductionmentioning

confidence: 99%

“…The potential of cfDNA in cancer monitoring has emerged since the advent of liquid biopsy [3]. In cancer studies, cfDNAs are widely investigated for genomics and epigenomics patterns due to the fact that they are representative of the primary tumor, as well as their non-invasive nature; therefore, they could be feasible biomarkers in the clinic for cancer detection and more precise treatments [1]. Among several cfDNA studies, the 5-hydroxymethylcytosine (5hmC) is a specific DNA modification used in cancer monitoring, however, there is less attention in pan-cancer studies [4,5].…”

Section: Introductionmentioning

confidence: 99%

The direct/inverse interplay among 5-hydroxymethylcytosine (5hmC)-related biological pathways in gastrointestinal pan-cancer study

Moraveji¹,

Khoshbakht²,

Mokhtari³

et al. 2021

Preprint

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5-hydroxymethylcytosine (5-hmC) as an epigenetics marker has significant impacts on cancer progression. Identification of preserved 5-hmC-related subnetworks in pan-cancer studies could lead to a better understanding of gastrointestinal (GI) cancers insights. Here, we conducted a network-based analysis on 5-hmC values of GI cancers, including colon, gastric, pancreatic cancers, and healthy donors. The co-5-hmC network was reconstructed using the weighted gene coexpression network (WGCNA) method. The hierarchical clustering method was implemented to detect pan-cancer-related modules/subnetworks. The preservation of modules was assessed using another dataset. Modules were functionally enriched, and biological pathways were visualized using the ConsensuspathDB. A 5-hmC predictive model was determined using the elastic network classifier to distinguish cancer patients and healthy individuals. To assess the efficiency of the model the recursive operating characteristics (AUC) curve was computed using the 5 cross-fold validation and an external dataset as well. Three pan-cancer-related subnetworks were detected preserved in another dataset. The main biological pathways were the cell cycle, apoptosis, and extracellular matrix (ECM) organization. The direct association between the cell cycle and ECM, the inverse association between apoptosis and ECM organization, and the inverse association between the cell cycle and ECM organization were detected for the 5-hmC marker in GI cancers. The AUC of 92% (0.73-1.00) was detected for the predictive model. In conclusion, the intricate association among biological pathways of ECM organization, Cell cycle, and apoptosis in GI cancers might be the consequence of epigenetics aberration; such findings could be beneficial in precision medicine using liquid biopsy in early stages.

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Cell‑free DNA as a liquid biopsy for early detection of gastric cancer (Review)

Cited by 23 publications

References 114 publications

Biomarkers for Gastric Cancer Screening and Early Diagnosis

Biomarkers for Gastric Cancer Screening and Early Diagnosis

Polymer Nanoparticles and Nanomotors Modified by DNA/RNA Aptamers and Antibodies in Targeted Therapy of Cancer

The direct/inverse interplay among 5-hydroxymethylcytosine (5hmC)-related biological pathways in gastrointestinal pan-cancer study

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