Cell Free DNA and Procalcitonin as Early Markers of Complications in ICU Patients with Multiple Trauma and Major Surgery

Ahmed, Asmaa Ismail; Soliman, Randa Aly; Samir, Shereif

doi:10.7754/clin.lab.2016.160615

Cited by 24 publications

(19 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the liver transplantation, hematoma, CMV infection, and acute rejection led to increased ddcfDNA fractions up to 90% compared to 10% during stable graft function [6]. Infection can also lead to an increase in level of ddcfDNA, and one study reported that a combination of ddcfDNA with PCT can distinguished the infection leading to the ddcfDNA increase [20].We detected human parvovirus B19 reads in recipient p19 at the 2nd post-operative day, and the dynamic increase in B19 reads over time. At last, the recipient was confirmed with human parvovirus B19 infection with low hemoglobin and recovered after treatment with IVIG.…”

Section: Discussionmentioning

confidence: 99%

Dynamics of early post-operative plasma ddcfDNA levels in kidney transplantation: a single-center pilot study

et al. 2018

View full text Add to dashboard Cite

Donor-derived cell-free DNA (ddcfDNA) is reported to be a promising noninvasive biomarker for acute rejection in organ transplant. However, studies on monitoring ddcfDNA dynamics during the early periods after organ transplantation are scarce. Our study assessed the dynamic variation in ddcfDNA in early period with various types and status of kidney transplantation. Target region capture sequencing used identifies ddcfDNA level in 21 kidney transplant recipients. Median ddcfDNA level was 20.69% at the initial time post-transplant, and decreased to 5.22% on the first day and stayed at the stable level after the second day. The ddcfDNA level in DCD (deceased donors) group (44.99%) was significantly higher than that in LDRT (living donor) group (10.24%) at initial time, P < 0.01. DdcfDNA level in DGF (delayed graft function) recipients was lower (23.96%) than that in non-DGF (47.74%) at the initial time, P = 0.89 (19.34% in DGF and 4.46% in non-DGF on the first day, P = 0.17). DdcfDNA level at initial time significantly correlated with serum creatinine (r = 0.219, P = 0.032) and warm ischemia time (r = 0.204, P = 0.040). Plasma ddcfDNA level decreased rapidly follow an L-shaped curve post-transplant, and level in DGF declined slower than non-DGF. The rebound of ddcfDNA level may indicate the occurrence of acute rejection.

show abstract

Section: Discussionmentioning

confidence: 99%

Dynamics of early post-operative plasma ddcfDNA levels in kidney transplantation: a single-center pilot study

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Cell‐free plasma DNA (cfDNA), also called extracellular DNA, comprises fragments of DNA that are released from apoptotic and necrotic cells . Elevated levels of cfDNA in blood have been found in various clinical conditions, such as cancer, trauma, stroke and sepsis . A few studies have indicated that cfDNA may have value in predicting the mortality of sepsis patients in intensive care units (ICUs) and in emergency department (ED) patients .…”

Section: Introductionmentioning

confidence: 99%

Plasma cell‐free DNA and qSOFA score predict 7‐day mortality in 481 emergency department bacteraemia patients

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Here, i.e., cfDNA, also known as extracellular DNA, which can be released by various host cells including neutrophils, macrophages, eosinophils, and tumor cells, as well as by certain strains of bacteria plays a significant role [77][78][79][80][81][82]. Elevated levels of cfDNA have been found in various pathologic conditions including trauma [83,84]. Interestingly, cfDNA that appears to exert both pro-and antifibrinolytic effects is a good predictor of trauma patient's outcome in the ICU [84].…”

Section: Dampsmentioning

confidence: 99%

“…Meanwhile, we are beginning to understand the large impact of cfDNA on posttraumatic inflammation. Release of cfDNA plays an important role in trauma [84,256]. The group around Hauser [256] has shown that traumatic injury causes a release of mtDAMPs into the circulation, which have functionally important immune consequences.…”

Section: Nucleic Acidsmentioning

confidence: 99%

Damage-associated molecular patterns in trauma

Relja

Land

2019

Eur J Trauma Emerg Surg

129

131

View full text Add to dashboard Cite

In 1994, the "danger model" argued that adaptive immune responses are driven rather by molecules released upon tissue damage than by the recognition of "strange" molecules. Thus, an alternative to the "self versus non-self recognition model" has been provided. The model, which suggests that the immune system discriminates dangerous from safe molecules, has established the basis for the future designation of damage-associated molecular patterns (DAMPs), a term that was coined by Walter G. Land, Seong, and Matzinger. The pathological importance of DAMPs is barely somewhere else evident as in the posttraumatic or post-surgical inflammation and regeneration. Since DAMPs have been identified to trigger specific immune responses and inflammation, which is not necessarily detrimental but also regenerative, it still remains difficult to describe their "friend or foe" role in the posttraumatic immunogenicity and healing process. DAMPs can be used as biomarkers to indicate and/or to monitor a disease or injury severity, but they also may serve as clinically applicable parameters for optimized indication of the timing for, i.e., secondary surgeries. While experimental studies allow the detection of these biomarkers on different levels including cellular, tissue, and circulatory milieu, this is not always easily transferable to the human situation. Thus, in this review, we focus on the recent literature dealing with the pathophysiological importance of DAMPs after traumatic injury. Since dysregulated inflammation in traumatized patients always implies disturbed resolution of inflammation, so-called model of suppressing/inhibiting inducible DAMPs (SAMPs) will be very briefly introduced. Thus, an update on this topic in the field of trauma will be provided.

show abstract

Cell Free DNA and Procalcitonin as Early Markers of Complications in ICU Patients with Multiple Trauma and Major Surgery

Abstract: cfDNA is a good predictor of patient outcome in ICU and to a lesser extent as a marker of sepsis. PCT is another promising marker that can complement cfDNA to reach better patient management. Other markers can help in less severe cases.

Cited by 24 publications

References 0 publications

Dynamics of early post-operative plasma ddcfDNA levels in kidney transplantation: a single-center pilot study

Dynamics of early post-operative plasma ddcfDNA levels in kidney transplantation: a single-center pilot study

Plasma cell‐free DNA and qSOFA score predict 7‐day mortality in 481 emergency department bacteraemia patients

Damage-associated molecular patterns in trauma

Contact Info

Product

Resources

About