Cell-Free Culture Supernatant of Probiotic Lactobacillus fermentum Protects Against H2O2-Induced Premature Senescence by Suppressing ROS-Akt-mTOR Axis in Murine Preadipocytes

Kumar, Ravi; Sharma, Anamika; Gupta, Mahesh; Padwad, Yogendra; Sharma, Rohit

doi:10.1007/s12602-019-09576-z

Cited by 41 publications

(26 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Alterations in these pathways have been reported in different MtS models, including increases, decreases and no modifications in their activities [53,[57][58][59]. Even though only few inconsistent results were reported regarding the effect of probiotics and synbiotics on those pathways [60][61][62][63][64][65][66], their role in the phosphorylation and subsequent activation of nNOS is well demonstrated [27,51,67,68]. We found that the inhibition of either PKC or PI3K with calfostin C or LY294002 respectively reduced EFS-induced NO release in a similar extent in the three experimental groups.…”

Section: Discussionmentioning

confidence: 99%

Beneficial Effect of a Multistrain Synbiotic Prodefen® Plus on the Systemic and Vascular Alterations Associated with Metabolic Syndrome in Rats: The Role of the Neuronal Nitric Oxide Synthase and Protein Kinase A

et al. 2020

View full text Add to dashboard Cite

A high fat diet (HFD) intake is crucial for the development and progression of metabolic syndrome (MtS). Increasing evidence links gut dysbiosis with the metabolic and vascular alterations associated with MtS. Here we studied the use of a combination of various probiotic strains together with a prebiotic (synbiotic) in a commercially available Prodefen® Plus. MtS was induced by HFD (45%) in male Wistar rats. Half of the MtS animals received Prodefen® Plus for 4 weeks. At 12 weeks, we observed an increase in body weight, together with the presence of insulin resistance, liver steatosis, hypertriglyceridemia and hypertension in MtS rats. Prodefen® Plus supplementation did not affect the body weight gain but ameliorated all the MtS-related symptoms. Moreover, the hypertension induced by HFD is caused by a diminished both nitric oxide (NO) functional role and release probably due to a diminished neuronal nitric oxide synthase (nNOS) activation by protein kinase A (PKA) pathway. Prodefen® Plus supplementation for 4 weeks recovered the NO function and release and the systolic blood pressure was returned to normotensive values as a result. Overall, supplementation with Prodefen® Plus could be considered an interesting non-pharmacological approach in MtS.

show abstract

Section: Discussionmentioning

confidence: 99%

Beneficial Effect of a Multistrain Synbiotic Prodefen® Plus on the Systemic and Vascular Alterations Associated with Metabolic Syndrome in Rats: The Role of the Neuronal Nitric Oxide Synthase and Protein Kinase A

et al. 2020

View full text Add to dashboard Cite

show abstract

“…It was observed that supplementation of secretory metabolites of probiotic Lactobacillus fermentum sufficiently rescued cells from stress-induced senescence as evident by a decrease in p16 Ink4a /p53/p21 WAF1 expression, markers of SASP, NF-κB activation and ROS-induced DNA damage. 100 These effects of probiotic bacteria were correlated with the inhibition of PI3K/Akt/mTOR pathway, which is rapidly emerging as a central regulator of senescence 101 and longevity, 102 thereby indicating that probiotic bacteria may suppress senescence by inhibiting aberrant mTOR activation and maintaining oxidative homeostasis. Together, these observations suggest that probiotic bacteria can influence the progression and severity of cell senescence which may have a major impact on tissue homeostasis and also provide mechanisms governing the known health-promoting effects of probiotics during aging (Figure 2).…”

Section: Probiotics As Modulators Of Cellular Senescencementioning

confidence: 99%

Probiotic bacteria as modulators of cellular senescence: emerging concepts and opportunities

Sharma

Padwad

2019

Gut Microbes

Self Cite

View full text Add to dashboard Cite

Probiotic bacteria are increasingly gaining importance in human nutrition owing to their multifaceted health beneficial effects. Studies have also shown that probiotic supplementation is useful in mitigating age-associated oxi-inflammatory stress, immunosenescence, and gut dysbiosis thereby promoting health and longevity. However, our current understanding of the process of aging suggests a strong interrelationship between the accumulation of senescent cells and the development of aging phenotype, including the predisposition to age-related disorders. The present review studies the documented pro-longevity effects of probiotics and highlights how these beneficial attributes of probiotics could be related to the mitigation of cellular senescence. We present a perspective that to fully understand and comprehend the anti-aging characteristics of probiotic bacteria; it is imperative that probiotics or their synbiotic amalgamation with plant polyphenols, be studied under the purview of cellular senescence, that may ultimately help devise probiotic-based anti-senescence strategies.

show abstract

“…It is speculated that probiotics can blunt the progression of aging processes through the possible factors that govern age-related changes of the gut microbiome, age-related hallmarks (e.g., mitochondria dysfunction, telomere attrition), impact the severity and progression of cellular senescence, oxidative stress, epigenetic alteration, alteration of intracellular and intercellular communication, deprivation of proteostasis and including others [133][134][135]. For example, Kumar and co-workers recently reported the anti-senescence potential of secretory metabolites of L. fermentum that mitigated the formation and severity of stress-induced senescence by a mechanism that relied on the downregulated phosphorylation of the PI3K-Akt-mTOR pathway and modulated the senescence-associated biomarkers such as p21WAF1, p38MAPK, p53, SA-β-gal, iNOS, COX-2, NF-κB and DNA damage response in 3T3-L1 preadipocytes [136]. However, these remarks were claimed and observed only in several in vitro and in vivo studies, excluding human subjects [137,138].…”

Section: The Impact Of Probiotics On Longevity the New Concept Of "Gerobiotics"mentioning

confidence: 99%

Novel Insights into the Role of Probiotics in Respiratory Infections, Allergies, Cancer, and Neurological Abnormalities

et al. 2021

View full text Add to dashboard Cite

In recent years, probiotics have attracted public attention and transformed the social perception of microorganisms, convening a beneficial role/state on human health. With aging, the immune system, body physiology, and intestinal microbiota tend to change unfavorably, resulting in many chronic conditions. The immune-mediated disorders can be linked to intestinal dysbiosis, consequently leading to immune dysfunctions and a cluster of conditions such as asthma, autoimmune diseases, eczema, and various allergies. Probiotic bacteria such as Lactobacillus and Bifidobacterium species are considered probiotic species that have a great immunomodulatory and anti-allergic effect. Moreover, recent scientific and clinical data illustrate that probiotics can regulate the immune system, exert anti-viral and anti-tumoral activity, and shields the host against oxidative stress. Additionally, microbiota programming by probiotic bacteria can reduce and prevent the symptoms of respiratory infections and ameliorate the neurological status in humans. This review describes the most recent clinical findings, including safe probiotic therapies aiming to medicate respiratory infections, allergies, cancer, and neurological disorders due to their physiological interconnection. Subsequently, we will describe the major biological mechanism by which probiotic bacteriotherapy expresses its anti-viral, anti-allergic, anticancer, and neuro-stimulatory effects.

show abstract

Cell-Free Culture Supernatant of Probiotic Lactobacillus fermentum Protects Against H2O2-Induced Premature Senescence by Suppressing ROS-Akt-mTOR Axis in Murine Preadipocytes

Cited by 41 publications

References 63 publications

Beneficial Effect of a Multistrain Synbiotic Prodefen® Plus on the Systemic and Vascular Alterations Associated with Metabolic Syndrome in Rats: The Role of the Neuronal Nitric Oxide Synthase and Protein Kinase A

Beneficial Effect of a Multistrain Synbiotic Prodefen® Plus on the Systemic and Vascular Alterations Associated with Metabolic Syndrome in Rats: The Role of the Neuronal Nitric Oxide Synthase and Protein Kinase A

Probiotic bacteria as modulators of cellular senescence: emerging concepts and opportunities

Novel Insights into the Role of Probiotics in Respiratory Infections, Allergies, Cancer, and Neurological Abnormalities

Contact Info

Product

Resources

About