Cell-free cartilage engineering approach using hyaluronic acid–polycaprolactone scaffolds: A study <i>in vivo</i>

Lebourg, Myriam; Martínez-Díaz, Santos; García-Giralt, Natàlia; Torres-Claramunt, Raúl; Ribelles, JL Gómez; Vilà-Canet, Gemma; Monllau, J.C.

doi:10.1177/0885328213507298

Cited by 32 publications

(30 citation statements)

References 27 publications

(33 reference statements)

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“…The reason may be that the MSC‐secreted extracellular matrix deposited on the scaffold forms a barrier to affect the macrophage‐led remodeling in vivo . Similar results were found in cartilage regeneration with cell‐free hyaluronic acid‐polycaprolactone scaffolds . More and more studies have proved that the mechanical property of the matrix in which cells reside is an important regulator for stem cell fate .…”

Section: Introductionsupporting

confidence: 77%

Cell‐free scaffolds with different stiffness but same microstructure promote bone regeneration in rabbit large bone defect model

Chen

Yang

2015

J Biomedical Materials Res

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To promote bone healing, bone repair biomaterials are increasingly designed to incorporate growth factors. However, the impact of matrix mechanics of cell-free scaffold independent of microstructure on the osteogenic differentiation of endogenous osteoprogenitor cells orchestrating bone repair and regeneration remains not to be fully understood. In our recent study, three-dimensional (3D) scaffolds with different stiffness but same microstructure have been successfully fabricated by coating decellularized bone with collagen/hydroxyapatite (HA) mixture with different collagen rations. It has been demonstrated that the scaffold with optimal stiffness can induce the osteogenic differentiation of MSCs in vitro and in the subcutaneous tissue. The present in vivo study further investigated the repair efficiency of these scaffolds in a rabbit radius with a critical-sized segmental defect model and its potential mechanism. Micro-computed tomography (μ-CT), X-ray and histological analysis were carried out to evaluate the repair capacity of these scaffolds. The results demonstrated that the cell-free scaffold with optimal stiffness incorporation of endogenous osteoprogenitor cells significantly promoted the repair and reconstruction quality of mass bone defect. One of the crucial mechanisms was that hypoxia and stromal cell-derived factor-1α (SDF-1α) mediated mesenchymal stem cells (MSCs) migration by which matrix mechanics exerted influence on bone fracture healing. These findings suggested that only modulating the matrix stiffness of cell-free scaffold can be one of the most attractive strategies for promoting the progression of bone healing.

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Section: Introductionsupporting

confidence: 77%

Cell‐free scaffolds with different stiffness but same microstructure promote bone regeneration in rabbit large bone defect model

Chen

Yang

2015

J Biomedical Materials Res

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“…The effect of fibrin clot in our “ in vitro ” experiments must be analyzed since it mimics the physiological conditions observed when a scaffold is implanted in a cartilage defect combined with microfracture of subchondral bone. Bleeding in the zone of the implant originates a clot which fills the scaffold pores, acting as migration path for mesenchymal stem cells coming from subchondral bone . On the other hand it has been demonstrated that fibrin encapsulation play a positive role in mechanotransduction, possibly simulating the necessary pericellular environment for mechanosensitive cells .…”

Section: Resultsmentioning

confidence: 99%

Mechanical fatigue performance of PCL‐chondroprogenitor constructs after cell culture under bioreactor mechanical stimulus

et al. 2015

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In tissue engineering of cartilage, polymeric scaffolds are implanted in the damaged tissue and subjected to repeated compression loading cycles. The possibility of failure due to mechanical fatigue has not been properly addressed in these scaffolds. Nevertheless, the macroporous scaffold is susceptible to failure after repeated loading-unloading cycles. This is related to inherent discontinuities in the material due to the micropore structure of the macropore walls that act as stress concentration points. In this work, chondrogenic precursor cells have been seeded in poly-e-caprolactone (PCL) scaffolds with fibrin and some were submitted to free swelling culture and others to cyclic loading in a bioreactor. After cell culture, all the samples were analyzed for fatigue behavior under repeated loading-unloading cycles. Moreover, some components of the extracellular matrix (ECM) were identified. No differences were observed between samples undergoing free swelling or bioreactor loading conditions, neither respect to matrix components nor to mechanical performance to fatigue. The ECM did not achieve the desired preponderance of collagen type II over collagen type I which is considered the main characteristic of hyaline cartilage ECM. However, prediction in PCL with ECM constructs was possible up to 600 cycles, an enhanced performance when compared to previous works. PCL after cell culture presents an improved fatigue resistance, despite the fact that the measured elastic modulus at the first cycle was similar to PCL with poly(vinyl alcohol) samples. This finding suggests that fatigue analysis in tissue engineering constructs can provide additional information missed with traditional mechanical measurements. AbstractIn tissue engineering of cartilage, polymeric scaffolds are implanted in the damaged tissue and subjected to repeated compression loading cycles. The possibility of failure due to mechanical fatigue has not been properly addressed in these scaffolds. Nevertheless, the macroporous scaffold is susceptible to failure after repeated loading-unloading cycles. This is related to inherent discontinuities in the material due to the micropore structure of the macro-pore walls that act as stress concentration points. In this work, chondrogenic precursor cells have been seeded in Poly-ε-caprolactone (PCL) scaffolds with fibrin and some were submitted to free swelling culture and others to cyclic loading in a bioreactor. After cell culture, all the samples were analyzed for fatigue behavior under repeated loading-unloading cycles. Moreover, some components of the extracellular matrix (ECM) were identified. No differences were observed 2 between samples undergoing free swelling or bioreactor loading conditions, neither respect to matrix components nor to mechanical performance to fatigue. The ECM did not achieve the desired preponderance of collagen type II over collagen type I which is considered the main characteristic of hyaline cartilage ECM. However, prediction in PCL with ECM constructs was possible u...

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“…HA is a class of specific linear glycosaminoglycan, which is secreted by synovium B cell intra-articular [11,[18][19][20][21]. HA plays important roles in lubrication of synovial joint, protection of cartilaginous and maintenance of cartilage viscoelastic.…”

Section: Discussionmentioning

confidence: 99%

Effects of hemarthrosis on cartilage and synovium in rabbits

Zhao

Chen

et al. 2016

Eur J Trauma Emerg Surg

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Cell-free cartilage engineering approach using hyaluronic acid–polycaprolactone scaffolds: A study in vivo

Cited by 32 publications

References 27 publications

Cell‐free scaffolds with different stiffness but same microstructure promote bone regeneration in rabbit large bone defect model

Cell‐free scaffolds with different stiffness but same microstructure promote bone regeneration in rabbit large bone defect model

Mechanical fatigue performance of PCL‐chondroprogenitor constructs after cell culture under bioreactor mechanical stimulus

Effects of hemarthrosis on cartilage and synovium in rabbits

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