Cell-Fiber Interactions on Aligned and Suspended Nanofiber Scaffolds

Sheets, Kevin; Ji, Wang; Meehan, Sean; Sharma, Puja; Ng, Colin Uber; Khan, Mohammad Sohail; Koons, Brian; Behkam, Bahareh; Nain, Amrinder S.

doi:10.1166/jbt.2013.1105

Cited by 23 publications

(27 citation statements)

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“…Although substantial research has been performed to characterize the response of skeletal myoblasts to engineered directionality, the mechanisms underlying cellular behavior in response to topographical cues remain to be clarified. [109–112] Creating awareness of and understanding mechanisms underlying the effect of aligned materials on myogenesis is critical to improving our collective approach to skeletal muscle tissue engineering. Furthermore, understanding the mechanisms driving myogenesis within in vitro platforms will allow for design and development of scaffolds that can recruit host cells or initiate endogenous tissue regeneration cascades in situ , which may prove to be a superior approach to the delivery of cells via scaffolds.…”

Section: Mechanisms Of Cellular Responses To Anisotropic Materialsmentioning

confidence: 99%

“…[131, 132] The role of filopodia in cell-cell interactions between myoblasts and/or myotubes remains to be elucidated, but in the context of anisotropic materials, cell-cell interactions depend mainly on cell density, separation between features that provide directional cues, alignment and cell types. [112] If the cell density is high enough that filopodia may engage, cell-cell interaction may take place via filopodial projections. Expression of cytoskeleton regulators in cells cultured on aligned versus randomly oriented nanofibers was probed using a microarray.…”

Section: Mechanisms Of Cellular Responses To Anisotropic Materialsmentioning

confidence: 99%

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Anisotropic Materials for Skeletal‐Muscle‐Tissue Engineering

2016

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Repair of damaged skeletal muscle tissue is limited by the regenerative capacity of native tissue. Current clinical approaches are not optimal for treatment of large volumetric skeletal muscle loss. As an alternative, tissue engineering represents a promising approach for the functional restoration of damaged muscle tissue. A typical tissue engineering process involves the design and fabrication of a scaffold that closely mimics the native skeletal muscle extracellular matrix allowing for organization of cells into a physiologically relevant, 3D architecture. In particular, anisotropic materials, which mimic the morphology of the native skeletal muscle ECM, can be fabricated using various biocompatible materialsto guide cell alignment, elongation, proliferation and differentiation into myotubes. In this article, we first provide an overview of fundamental concepts associated with muscle tissue engineering and the current status of the muscle tissue engineering approaches. We then review recent advances in development of anisotropic scaffolds with micro- or nano-scale features and examine how scaffold topographical, mechanical, and biochemical cues correlate to observed cellular function and phenotype development. Finally, we highlight some recent developments in both the design and utility of anisotropic materials in skeletal muscle tissue engineering along with their potential impact on future research and clinical application.

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Section: Mechanisms Of Cellular Responses To Anisotropic Materialsmentioning

confidence: 99%

Section: Mechanisms Of Cellular Responses To Anisotropic Materialsmentioning

confidence: 99%

Anisotropic Materials for Skeletal‐Muscle‐Tissue Engineering

2016

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“…Fibers were spun on scaffolds using the STEP technique (US9029149 B2 patent) to manufacture multi-layered, aligned suspended nanofibers in parallel or perpendicular orientations [24][25][26]. Briefly, the polymer is pushed through a syringe pump and stretched out into a continuous fiber as the spinneret comes in contact with the rotating frame and the solvent evaporates.…”

Section: Scaffold Manufacturing and Characterizationmentioning

confidence: 99%

Aligned Nanofiber Topography Directs the Tenogenic Differentiation of Mesenchymal Stem Cells

2017

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Abstract:Tendon is commonly injured, heals slowly and poorly, and often suffers re-injury after healing. This is due to failure of tenocytes to effectively remodel tendon after injury to recapitulate normal architecture, resulting in poor mechanical properties. One strategy for improving the outcome is to use nanofiber scaffolds and mesenchymal stem cells (MSCs) to regenerate tendon. Various scaffold parameters are known to influence tenogenesis. We designed suspended and aligned nanofiber scaffolds with the hypothesis that this would promote tenogenesis when seeded with MSCs. Our aligned nanofibers were manufactured using the previously reported non-electrospinning Spinneret-based Tunable Engineered Parameters (STEP) technique. We compared parallel versus perpendicular nanofiber scaffolds with traditional flat monolayers and used cellular morphology, tendon marker gene expression, and collagen and glycosaminoglycan deposition as determinants for tendon differentiation. We report that compared with traditional control monolayers, MSCs grown on nanofibers were morphologically elongated with higher gene expression of tendon marker scleraxis and collagen type I, along with increased production of extracellular matrix components collagen (p = 0.0293) and glycosaminoglycan (p = 0.0038). Further study of MSCs in different topographical environments is needed to elucidate the complex molecular mechanisms involved in stem cell differentiation.

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“…[45][46] The growth, size, maturity and number of FAs depend on morphology, mechanical and biophysiochemical properties of substrates. Reports indicate that the number of FAs on a planar surface is more than on the substrates made of nanofibers.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Model of a Fibrosa Layer of a Heart Valve

Jana

Lerman

Simari³

2015

ACS Appl. Mater. Interfaces

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The fibrosa layer of a cardiac aortic valve is composed mostly of a dense network of type I collagen fibers oriented in circumferential direction. This main layer bears the tensile load and responds to the high stress on a leaflet. The inner fibrosa layer is also the site of pathophysiologic changes that result in valvular dysfunction, including stenosis and regurgitation. In vitro studies of these changes are limited by the absence of a substrate that mimics the circumferentially oriented structure of the fibrosa layer. In heart valve tissue engineering, generation of this layer is challenging. This study aimed to develop an artificial fibrosa layer of a native aortic leaflet. A unique morphologically biomimicked, pliable, but standalone substrate with circumferentially oriented nanofibers was fabricated by electrospinning on a novel collector designed for this study. The substrate had low-bulk tensile stiffness and ultimate strength; thus, cultured valvular interstitial cells (VICs) showed a fibroblast phenotype that is generally observed in a healthy aortic leaflet. Furthermore, gene and protein expression and morphology of VICs in substrates were close to those in the fibrosa layer of a native aortic leaflet. This artificial fibrosa layer can be useful for in vitro studies of valvular dysfunctions.

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Cell-Fiber Interactions on Aligned and Suspended Nanofiber Scaffolds

Cited by 23 publications

References 0 publications

Anisotropic Materials for Skeletal‐Muscle‐Tissue Engineering

Anisotropic Materials for Skeletal‐Muscle‐Tissue Engineering

Aligned Nanofiber Topography Directs the Tenogenic Differentiation of Mesenchymal Stem Cells

In Vitro Model of a Fibrosa Layer of a Heart Valve

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