Cell Fate Switch during In Vitro Plant Organogenesis

Abstract

Plant mature cells have the capability to reverse their state of differentiation and produce new organs under cultured conditions. Two phases, dedifferentiation and redifferentiation, are commonly characterized during in vitro organogenesis. In these processes, cells undergo fate switch several times regulated by both extrinsic and intrinsic factors, which are associated with reentry to the cell cycle, the balance between euchromatin and heterochromatin, reprogramming of gene expression, and so forth. This short article reviews the advances in the mechanism of organ regeneration from plant somatic cells in molecular, genomic and epigenetic aspects, aiming to provide important information on the mechanism underlying cell fate switch during in vitro plant organogenesis.

Germinación y micropropagación de tomate de cáscara (Physalis ixocarpa) tetraploide

“…Also in this case, the root system developed spontaneously eliminating the need of a medium with auxin before acclimatization. According to Zhao et al (2008) eliminando la necesidad de medio con auxinas antes de la aclimatización. De acuerdo con Zhao et al (2008) una relación alta entre citocinina y auxina promueve la regeneración de raíz.…”

Immature Zygotic Embryo Cultures of Arabidopsis. A Model System for Molecular Studies on Morphogenic Pathways Induced In Vitro

“…Molecular events related to shoot ORG induced in vitro have been analyzed in cultures of root explants (Barton and Poethig, 1993;Ozawa et al, 1998;Cary et al, 2001;Cary et al, 2002;Zuo et al, 2002;Gordon et al, 2007;Zhao et al, 2008;Bao et al, 2009). To reveal the molecular mechanisms involved in the embryogenic transition and somatic embryo formation, culture of IZE explants of Arabidopsis has been recommended (Gaj, 2004) and successfully applied (Gaj et al, 2005;Su et al, 2009).…”

Reprogramming adult cells during organ regeneration in forest species

“…Sena et al 7 have described in Arabidopsis thaliana that a reversion to stem cell niche activity is not necessary for in vivo early-root tip regeneration from developmentally young cells that have undergone only few divisions after the asymmetric division of a stem cell, and a set of markers associated with the competence of tissues is reported. However, Zhao et al 8 describe the formation of a stem cell niche from competent callus cells obtained from young seedlings during in vitro plant organogenesis. According to Birnbaum and Sánchez-Alvarado, 2 there could be many possible pathways and strategies from early to later stages of regeneration in both animals and plants depending on the progenitor cells.…”