Cell Envelope Phospholipid Composition of Burkholderia multivorans

Abstract

Burkholderia multivorans causes opportunistic pulmonary infections in cystic fibrosis and immunocompromised patients. The purpose of the present study was to determine the nature of the phospholipids and their fatty acid constituents comprising the cell envelope membranes of strains isolated from three disparate sources. A conventional method for obtaining the readily extractable lipids fraction from bacteria was employed to obtain membrane lipids for thin-layer chromatographic and gas chromatography-mass spectrophotometric analyses. Major fatty acid components of the B. multivorans readily extractable lipid fractions included C(16:0) (palmitic acid), C(16:1) (palmitoleic acid), and C(18:1) (oleic acid), while C(14:0) (myristic acid), ΔC(17:0) (methylene hexadecanoic acid), C(18:0) (stearic acid), and ΔC(19:0) (methylene octadecanoic acid) were present in lesser amounts. Fatty acid composition differed quantitatively among strains with regard to C(16:0), C(16:1), ΔC(17:0), C(18:1), and ΔC(19:0) with the unsaturated:saturated fatty acid ratios being significantly less in a cystic fibrosis type strain than either environmental or chronic granulomatous disease strains. Phospholipids identified in all B. multivorans strains included lyso-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol in similar ratios. These data support the conclusion that the cell envelope phospholipid profiles of disparate B. multivorans strains are similar, while their respective fatty acyl substituent profiles differ quantitatively under identical cultivation conditions.

Identification and characterization of Burkholderia multivorans CCA53

“…The unsaturated fatty acids C 16:1 ω6c, C 16:1 ω7c, C 18:1 ω6c and C 18:1 ω7c were major components of B. multivorans CCA53. This fatty acid profile conformed to the profiles of B. multivorans ATCC 17616 [32] and B. multivorans CGD2 [32]. …”

Metabolomic profiling of Burkholderia cenocepacia in synthetic cystic fibrosis sputum medium reveals nutrient environment-specific production of virulence factors

“…S11 b). Production of cyclopropane acids have been previously reported in B. multivorans 107 . Cyclopropane fatty acids such as phytomonic acid are suggested to regulate membrane fluidity and stability and have been shown to increase extracellular survival in acidic or under conditions of high osmolarity 108 , 109 .…”

Membrane‐Vesicle‐Mediated Interbacterial Communication Activates Silent Secondary Metabolite Production

“…[19][20][21] In scanning electron microscope (SEM) images, B. multivorans (around 500 nm in length) budded MVs with a diameter of around 100 nm (Figure 4a, Figure S1b), which were fractionated by ultracentrifugation according to previous reports. [13,22] GC-MS analyses of membrane lipid compositions showed that the most abundant fatty acid in the MVs was palmitic acid, which is also a major component of the outer membrane of B. multivorans [23] (Figure S1e). Bacterial MVs usually accumulate unique fatty acids containing cyclopropane rings as membrane components.…”