Cell Envelope of an Iron-Oxidizing Bacterium: Studies of Lipopolysaccharide and Peptidoglycan

Wang, W. S.; Korcynski, M. S.; Lundgren, D. G.

doi:10.1128/jb.104.1.556-565.1970

Cited by 23 publications

(11 citation statements)

References 10 publications

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“…In addition to phosphatidyl ethanolamine, phosphatidyl glyceroland phosphatidyl serine were used as references. They both had a slower migration rate than phosphatidyl ethanolamine and did not correspond to any of the spots seen on sulfuric the same as those of the sugar analysis described previously and also described byWang et al (1970). The results of this analysis are shown inTable 942%) and stearic acid (0.19%).…”

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confidence: 71%

Characterization of an immunizing antigen with endotoxin-like properties isolated from Pasteurella multocida

Ganfield

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The Antigens and Toxins of Pasteurella multocida 4 Bacterial Toxins Extracellular or Free Endotoxins 18 MATERIALS Sources of Chemicals and Biochemicals Source of Animals Equipment METHODS Bacteriological Methods Isolation of the Immunogenic and Toxic Complex 27 Chemical Methods Physical Methods 35 Biological Methods 45 Serological Methods 49 RESULTS 51 Isolation Procedures 52 Biological and Serological Studies of the 40,000 rpm Preparations 55 Chemical Analysis of the Toxic and Immunogenic 40-P Preparations 69 Isoelectric Focusing Experiments with the 40-P Preparations 83 iii Page Fractionation by Gel Filtration Chromatography The Results of Biological Studies of the Gel Filtration Fractions Particle Size Determination of the Gel Filtration Fraction S-II Estimation of the Particle Weight of the Sepharose 2B Fraction S-II Electrophoretic Studies on the Sepharose 2B Fraction S-II DISCUSSION OF RESULTS 161 Comparison of These Results with Other P. multocida Studies 174 SUMMARY 179 LITERATURE CITED 183 ACKNOWLEDGEMENTS 195 P. multocida. Both encapsulated, virulent and nonencapsulated, avirulent forms of P. multocida have been described by Heddleston (1962) and both forms of the organisms have been found to be highly immunogenic in chickens when used as killed bacterins. The substance characterized in this investigation was isolated from saline extracts of the avirulent nonencapsulated P. multocida strain P-1059 Gray. Since this organism did not have a capsule, it was felt it would be easier to isolate and characterize the immunogenic substance. The P-1059 strain (originally a turkey isolate) has been described by Heddleston and co-workers (1962, 1966) and is considered to represent the most prevalent P. multocida immunogenic type found in field cases of fowl cholera.

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confidence: 71%

Characterization of an immunizing antigen with endotoxin-like properties isolated from Pasteurella multocida

Ganfield

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“…spp. (10,19,21,27,28,30). The goals of this study were to develop a method to study LPS chemotypes of thiobacilli grown in tailings and in synthetic media, and to determine whether LPS heterogeneity exists among Thiobacillus spp.…”

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Examination of Lipopolysaccharide (O-Antigen) Populations of Thiobacillus ferrooxidans from Two Mine Tailings

Southam

Beveridge

1993

Appl Environ Microbiol

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Net acid-generating capacities of 39.74 kg of H2SO4 per ton (ca. 0.05 kg/kg) (pH 2.68) for the Lemoine copper mine tailings (closed ca. 8 years ago; located 40 km west of Chibougamau, Quebec, Canada) and 16.07 kg of H2SO4 per ton (ca. 0.02 kg/kg) (pH 3.01) for the Copper Rand tailings (in current use and 50 km distant [east] from those of Lemoine) demonstrate that these sulfide tailings can support populations of acidophilic thiobacilli. Oxidized regions in both tailings environments were readily visible, were extremely acidic (Lemoine, pH 2.36; Copper Rand, pH 3.07), and provided natural isolates for our study. A 10%Yo (wt/vol) oxalic acid treatment, which solubilizes both ferric sulfate and ferric hydroxide precipitates (B. Ramsay, J. Ramsay, M. deTremblay, and C. Chavarie, Geomicrobiol. J. 6:171-177, 1988), enabled the recovery of intact bacterial cells from the tailings material and from liquid synthetic medium for lipopolysaccharide analysis. No viable cells could be cultured after this oxalic acid treatment. Sodium dodecyl sulfate-polyacrylamide gel electro

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“…The effect of this cation on some of their biological properties and the conditions that led to the possible release of Cu2" from the complex were studied. Other cations, however, are also present in cell walls and in LPS (3,5,8,23) and, under certain conditions, may influence their properties. It is not clear to what extent they are structural components of the complex with a definite function or are non-specifically adsorbed as a consequence of contamination during the course of the isolation and purification procedures.…”

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confidence: 99%

Effects of Certain Cations (Fe, Zn, Mg, and Ca) on Bacterial Endotoxins

1978

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The natural occurrence of cations Fe, Zn, Mg, and Ca in the lipopolysaccharide (LPS) of both the S and R forms of Shigella dysenteriae 1 was studied. LPS preparations were obtained either by phenol-water extraction (according to the method of Westphal et al., Z. Naturforsch. 7b:148-155, 1952) or by extraction of cells with hypertonic sodium chloride-sodium citrate (according to the method of Raynaud and Digeon, C. R. Acad. Sci. (Paris) 229:564-566,1949), with subsequent chromatographic purification on Sephadex G200 and Sepharose 4B columns. The cation in highest concentration in the Westphal extract was Mg2+ (as much as 30 ,ug/mg), and the lowest one was Fe (ca. 0.10 ,ug/mg). In LPS of the Raynaud type, the cation in highest concentration was Ca2+ (as much as 13 ,ug/mg), and the lowest one was Fe (ca. 0.10 ,.g/mg). The effects of increasing and decreasing the concentrations of cations (Fe, Zn, Mg, Ca) upon the biological activity of the endotoxins was evaluated by using toxicity in mice and the Limulus test. It

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Cell Envelope of an Iron-Oxidizing Bacterium: Studies of Lipopolysaccharide and Peptidoglycan

Cited by 23 publications

References 10 publications

Characterization of an immunizing antigen with endotoxin-like properties isolated from Pasteurella multocida

Characterization of an immunizing antigen with endotoxin-like properties isolated from Pasteurella multocida

Examination of Lipopolysaccharide (O-Antigen) Populations of Thiobacillus ferrooxidans from Two Mine Tailings

Effects of Certain Cations (Fe, Zn, Mg, and Ca) on Bacterial Endotoxins

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