2020
DOI: 10.1101/2020.06.15.153916
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Cell entry of SARS-CoV-2 conferred by angiotensin-converting enzyme 2 (ACE2) of different species

Abstract: 1The outbreak of the severe acute respiratory syndrome coronavirus 2 2 (SARS-CoV-2) poses a huge threat to many countries around the world. However, 3 where is it origin and which animals are sensitive to cross-species transmission is 4 unclear. The interaction of virus and cell receptor is a key determinant of host range 5 for the novel coronavirus. Angiotensin-converting enzyme 2 (ACE2) is demonstrated 6 as the primary entry receptor for SARS-CoV-2. In this study, we evaluated the 7 SARS-CoV-2 entry mediated… Show more

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Cited by 9 publications
(12 citation statements)
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“…However, these analyses are limited as it does not determine expression biochemically on epithelial cells that make contact to the outside world [29]. Several groups have transfected variants of ACE2 in cells to analyze transduction by SARS-CoV-pseudo viruses, although informative these studies do not provide information on the natural expression pattern of ACE2 in susceptible hosts [30, 31]. A detailed understanding of the difference between animal species and cell-specific expression of ACE2 at the molecular level is essential, as this can provide valuable knowledge on potential hosts that can be susceptible to SARS-like coronaviruses.…”
Section: Discussionmentioning
confidence: 99%
“…However, these analyses are limited as it does not determine expression biochemically on epithelial cells that make contact to the outside world [29]. Several groups have transfected variants of ACE2 in cells to analyze transduction by SARS-CoV-pseudo viruses, although informative these studies do not provide information on the natural expression pattern of ACE2 in susceptible hosts [30, 31]. A detailed understanding of the difference between animal species and cell-specific expression of ACE2 at the molecular level is essential, as this can provide valuable knowledge on potential hosts that can be susceptible to SARS-like coronaviruses.…”
Section: Discussionmentioning
confidence: 99%
“… Tang et al (2020) conducted virus infectivity studies using HEK293T cells expressing ACE2 from 11 species of animals, and found that pangolin-ACE2 could mediate SARS-CoV-2 entry. Besides, ACE2 of Rhinolophus ferrumequinum cannot support the entry of SARS-CoV-2 that is a capability of ACE2 of Rhinolophus sinicus in experiments conducted by Tang et al (2020) and Zhou P. et al (2020) .…”
Section: Wildlife As the Potential Intermediate Hostsmentioning
confidence: 99%
“… Tang et al (2020) conducted virus infectivity studies using HEK293T cells expressing ACE2 from 11 species of animals, and found that pangolin-ACE2 could mediate SARS-CoV-2 entry. Besides, ACE2 of Rhinolophus ferrumequinum cannot support the entry of SARS-CoV-2 that is a capability of ACE2 of Rhinolophus sinicus in experiments conducted by Tang et al (2020) and Zhou P. et al (2020) . By using pseudotyping particles of SARS-CoV-2 Spike mimics particle entry and quantitative cell-cell fusion assay, Conceicao et al (2020) observed that pangolin-ACE2 sustained higher levels of entry than hACE2.…”
Section: Wildlife As the Potential Intermediate Hostsmentioning
confidence: 99%
“…Furthermore, N-glycosylation motifs (e.g., N53) are conserved within all species and could potentially attach to the S-protein [ 54 ]. Based on protein modeling approaches, extensive vertebrate diversity has been reported as potentially susceptible to SARS-CoV-2 [ 122 , 125 ]. However, further experimental data will need to be conducted to confirm the results of the ACE-2 sequencing data [ 122 ].…”
Section: Sars-cov-2 Spike Protein and Ace-2mentioning
confidence: 99%
“…Moreover, the ACE-2 receptors of pigs could potentially be used by SARS-CoV-2 [ 277 ]. It is speculated that pig ACE-2 receptors could genetically recombine to mediate SARS-CoV-2 entry into other species similar to other swine recombinant events [ 125 ].…”
Section: Animal Host Diversity Of Sars-cov-2mentioning
confidence: 99%