Cell entry of avian reovirus follows a caveolin-1-mediated and dynamin-2-dependent endocytic pathway that requires activation of p38 mitogen-activated protein kinase (MAPK) and Src signaling pathways as well as microtubules and small GTPase Rab5 protein.

Huang, Wei-Ru; Wang, Ying C.; I., Pei; Wang, Lai; Wang, Chi Y.; Lin, Chi; Liu, Hung-Jen

doi:10.1074/jbc.a111.257154

Cited by 14 publications

(22 citation statements)

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“…ARV has been reported to activate Ras, p38 MAPK, and src followed with caveolin-1 mediated endocytic pathway in dynamin-2 dependent [10]. ARV-induced phosphorylation of caveolin-1 (Tyr14) and dynamin-2 expression as well as Rac1 activation through activation of p38 MAPK and Src in the early stage of the virus life cycle is bene�cial for virus entry [10]. It was demonstrated that both p38 MAPK and Src are associated with cellular proteins caveolin-1 and dynamin-2.…”

Section: Caveolae/lipid Raft-dependent Endocytosismentioning

confidence: 99%

“…It was demonstrated that both p38 MAPK and Src are associated with cellular proteins caveolin-1 and dynamin-2. A novel role for p38 MAPK and Src signaling has been found to exert positive effects on caveolin 1 and dynamin 2 expressions, thereby enhancing ARV entry [10]. ARV traffics to early endosomes require the participation of microtubules and small GTPase Rab5 [10].…”

Section: Caveolae/lipid Raft-dependent Endocytosismentioning

confidence: 99%

“…A novel role for p38 MAPK and Src signaling has been found to exert positive effects on caveolin 1 and dynamin 2 expressions, thereby enhancing ARV entry [10]. ARV traffics to early endosomes require the participation of microtubules and small GTPase Rab5 [10]. In addition to regulation of virus entry, it has also been found that p38 MAPK activation is bene�cial for ARV replication [52].…”

Section: Caveolae/lipid Raft-dependent Endocytosismentioning

confidence: 99%

“…e cell endocytic mechanism also provides a route for virus internalization. Recent research has discovered that numerous viruses favor the endocytosis as clathrin-mediated endocytosis (CME) [2][3][4][5][6][7], caveolae/lipid ra-mediated endocytosis [8][9][10], macropinocytosis [11][12][13], and several other unusual pathways [14,15] by eliciting the endocytic signaling pathways. is review focuses on the elements that are involved in regulating the mechanism of virus entry and their traffic systems.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Signaling and Cellular Pathways for Virus Entry

Chi

Liu

2013

ISRN Virology

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The cell signaling plays a pivotal role in regulating cellular processes and is often manipulated by viruses as they rely on the functions offered by cells for their propagation. The first stage of their host life is to pass the genetic materials into the cell. Although some viruses can directly penetrate into cytosol, in fact, most virus entry into their host cells is through endocytosis. This machinery initiates with cell type specific cellular signaling pathways, and the signaling compounds can be proteins, lipids, and carbohydrates. The activation can be triggered in a very short time after virus binds on target cells, such as receptors. The signaling pathways involved in regulation of viral entry are wide diversity that often cross-talk between different endocytosis results. Furthermore, some viruses have the ability to use the multiple internalization pathways which leads to the regulation being even more complex. In this paper, we discuss some recent advances in our understanding of cellular pathways for virus entry, molecular signaling during virus entry, formation of endocytic vesicles, and the traffic.

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Section: Caveolae/lipid Raft-dependent Endocytosismentioning

confidence: 99%

Section: Caveolae/lipid Raft-dependent Endocytosismentioning

confidence: 99%

Section: Caveolae/lipid Raft-dependent Endocytosismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Signaling and Cellular Pathways for Virus Entry

Chi

Liu

2013

ISRN Virology

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“…A number of viruses have been proved to enter host cells via the clathrin-dependent endocytosis pathway (12,33,36). Many other viruses have also been reported to enter host cells through the caveola-dependent pathway (3,17).…”

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confidence: 99%

Bovine Ephemeral Fever Virus Uses a Clathrin-Mediated and Dynamin 2-Dependent Endocytosis Pathway That Requires Rab5 and Rab7 as Well as Microtubules

Cheng

Shih

Huang

et al. 2012

J Virol

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The specific cell pathways involved in bovine ephemeral fever virus (BEFV) cell entry have not been determined. In this work, colocalization of the M protein of BEFV with clathrin or dynamin 2 was observed under a fluorescence microscope. To better understand BEFV entry, we carried out internalization studies with a fluorescently labeled BEFV by using a lipophilic dye, 3,30-dilinoleyloxacarbocyanine perchlorate (DiO), further suggesting that BEFV uses a clathrin-mediated endocytosis pathway. Our results suggest that clathrin-mediated and dynamin 2-dependent endocytosis is an important avenue of BEFV entry. Suppression of Rab5 or Rab7a through the use of a Rab5 dominant negative mutant and Rab7a short hairpin RNA (shRNA) demonstrated that BEFV requires both early and late endosomes for endocytosis and subsequent infection in MDBK and Vero cells. Treatment of BEFV-infected cells with nocodazole significantly decreased the M protein synthesis and viral yield, indicating that microtubules play an important role in BEFV productive infection, likely by mediating trafficking of BEFV-containing endosomes. Furthermore, BEFV infection was strongly blocked by different inhibitors of endosomal acidification, suggesting that virus enters host cells by clathrin-mediated and dynamin 2-dependent endocytosis in a pH-dependent manner.

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Expression of caveolin‐1 in the interfollicular but not the follicle‐associated epithelial cells in the bursa of fabricius of chickens

Bódi

Minkó

Fölker

et al. 2017

Journal of Morphology

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The surface epithelium of the bursa of Fabricius consists of interfollicular (IFE) and follicleassociated epithelium (FAE). The IFE comprises (i) cylindrical-shaped secretory cells (SC) and (ii) cuboidal basal cells (BCs). The FAE provides histological and two-way functional connections between the bursal lumen and medulla of the follicle. We used a carbon solution and anticaveolin-1 (Cav-1) to study the endocytic activity of FAE. Carbon particles entered the intercellular space of FAE, but the carbon particles were not internalized by the FAE cells.

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Cell entry of avian reovirus follows a caveolin-1-mediated and dynamin-2-dependent endocytic pathway that requires activation of p38 mitogen-activated protein kinase (MAPK) and Src signaling pathways as well as microtubules and small GTPase Rab5 protein.

Abstract: Authors are urged to introduce these corrections into any reprints they distribute. Secondary (abstract) services are urged to carry notice of these corrections as prominently as they carried the original abstracts.

Cited by 14 publications

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Molecular Signaling and Cellular Pathways for Virus Entry

Molecular Signaling and Cellular Pathways for Virus Entry

Bovine Ephemeral Fever Virus Uses a Clathrin-Mediated and Dynamin 2-Dependent Endocytosis Pathway That Requires Rab5 and Rab7 as Well as Microtubules

Expression of caveolin‐1 in the interfollicular but not the follicle‐associated epithelial cells in the bursa of fabricius of chickens

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