Cell encapsulation: technical and clinical advances

Orive, Gorka; Santos, Edorta; Poncelet, Denis; Hernández, Rosa Marı́a; Pedraz, José Luís; Wahlberg, Lars U.; Vos, Paul de; Emerich, Dwaine F.

doi:10.1016/j.tips.2015.05.003

Cited by 157 publications

(102 citation statements)

References 93 publications

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“…Because of its inherent biocompatibility, alginate hydrogel has been recognized as a promising biomaterial for biomedical applications. 49 Other unique properties of alginates include modifiable immunosuppression 50 and degradation 51 properties, making alginate an FDA approved biomaterials. 52 …”

Section: Introductionmentioning

confidence: 99%

Dental and orofacial mesenchymal stem cells in craniofacial regeneration: The prosthodontist’s point of view

Ansari

Seagroves

Chen

et al. 2017

The Journal of Prosthetic Dentistry

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Of the available regenerative treatment options, craniofacial tissue regeneration using mesenchymal stem cells (MSCs) shows promise. The ability of stem cells to produce multiple specialized cell types along with their extensive distribution in many adult tissues have made them an attractive target for applications in tissue engineering. MSCs reside in a wide spectrum of postnatal tissue types and have been successfully isolated from orofacial tissues. These dental-or orofacial-derived MSCs possess self-renewal and multilineage differentiation capacities. The craniofacial system is composed of complex hard and soft tissues derived from sophisticated processes starting with embryonic development. Because of the complexity of the craniofacial tissues, the application of stem cells presents challenges in terms of the size, shape, and form of the engineered structures, the specialized final developed cells, and the modulation of timely blood supply while limiting inflammatory and immunological responses. The cell delivery vehicle has an important role in the in vivo performance of stem cells and could dictate the success of the regenerative therapy. Among the available hydrogel biomaterials for cell encapsulation, alginate-based hydrogels have shown promising results in biomedical applications. Alginate scaffolds encapsulating MSCs can provide a suitable microenvironment for cell viability and differentiation for tissue regeneration applications. This review aims to summarize current applications of dental-derived stem cell therapy and highlight the use of alginate-based hydrogels for applications in craniofacial tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Dental and orofacial mesenchymal stem cells in craniofacial regeneration: The prosthodontist’s point of view

Ansari

Seagroves

Chen

et al. 2017

The Journal of Prosthetic Dentistry

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“…The key feature of the microencapsulation technique is that cells are embedded in a semipermeable polymerized structure with the aim of protecting them from a host immune attack while allowing the diffusion of nutrients, oxygen, and metabolic products that ensure cell function and survival 18, 19. Primary human hepatocytes cultured on alginate microbeads in vitro for 3 days showed albumin and urea production.…”

Section: Implantable Technologies For Liver Therapiesmentioning

confidence: 99%

Liver tissue engineering: From implantable tissue to whole organ engineering

Mazza

Al‐Akkad

Rombouts

et al. 2017

Hepatology Communications

101

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The term “liver tissue engineering” summarizes one of the ultimate goals of modern biotechnology: the possibility of reproducing in total or in part the functions of the liver in order to treat acute or chronic liver disorders and, ultimately, create a fully functional organ to be transplanted or used as an extracorporeal device. All the technical approaches in the area of liver tissue engineering are based on allocating adult hepatocytes or stem cell‐derived hepatocyte‐like cells within a three‐dimensional structure able to ensure their survival and to maintain their functional phenotype. The hosting structure can be a construct in which hepatocytes are embedded in alginate and/or gelatin or are seeded in a pre‐arranged scaffold made with different types of biomaterials. According to a more advanced methodology termed three‐dimensional bioprinting, hepatocytes are mixed with a bio‐ink and the mixture is printed in different forms, such as tissue‐like layers or spheroids. In the last decade, efforts to engineer a cell microenvironment recapitulating the dynamic native extracellular matrix have become increasingly successful, leading to the hope of satisfying the clinical demand for tissue (or organ) repair and replacement within a reasonable timeframe. Indeed, the preclinical work performed in recent years has shown promising results, and the advancement in the biotechnology of bioreactors, ex vivo perfusion machines, and cell expansion systems associated with a better understanding of liver development and the extracellular matrix microenvironment will facilitate and expedite the translation to technical applications. (Hepatology Communications 2018;2:131–141)

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“…At the same time, the semi-permeable nature of the membrane prevents the grafted cells being exposed to host immune cells and antibodies, avoiding their destruction (Fig. 4) [158, 159]. Through the release of therapeutic proteins, the grafted encapsulated cells can modify a circumscribed brain microenvironment or the whole brain milieu when transplanted into the CSF, and provide clinical benefits [132, 160, 161].…”

Section: Microencapsulation Permits Use Of Allo- and Xeno-grafting Wimentioning

confidence: 99%

“…At present, alginates are regarded as the most suitable biomaterials for cell encapsulation due to their abundance and excellent biocompatibility properties [162, 163]. New polymers are being tested to be used as carriers and scaffolds for biomolecules and cell delivery in tissue engineering applications [159]. Encapsulation devices range from ‘microscale’ devices (100 nm–1 mm) to ‘macroscale’ (3–8 cm).…”

Section: Microencapsulation Permits Use Of Allo- and Xeno-grafting Wimentioning

confidence: 99%

Blood–brain barrier and foetal-onset hydrocephalus, with a view on potential novel treatments beyond managing CSF flow

Guerra

Blázquez

Rodríguez

2017

Fluids Barriers CNS

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Despite decades of research, no compelling non-surgical therapies have been developed for foetal hydrocephalus. So far, most efforts have pointed to repairing disturbances in the cerebrospinal fluid (CSF) flow and to avoid further brain damage. There are no reports trying to prevent or diminish abnormalities in brain development which are inseparably associated with hydrocephalus. A key problem in the treatment of hydrocephalus is the blood–brain barrier that restricts the access to the brain for therapeutic compounds or systemically grafted cells. Recent investigations have started to open an avenue for the development of a cell therapy for foetal-onset hydrocephalus. Potential cells to be used for brain grafting include: (1) pluripotential neural stem cells; (2) mesenchymal stem cells; (3) genetically-engineered stem cells; (4) choroid plexus cells and (5) subcommissural organ cells. Expected outcomes are a proper microenvironment for the embryonic neurogenic niche and, consequent normal brain development.

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Cell encapsulation: technical and clinical advances

Cited by 157 publications

References 93 publications

Dental and orofacial mesenchymal stem cells in craniofacial regeneration: The prosthodontist’s point of view

Dental and orofacial mesenchymal stem cells in craniofacial regeneration: The prosthodontist’s point of view

Liver tissue engineering: From implantable tissue to whole organ engineering

Blood–brain barrier and foetal-onset hydrocephalus, with a view on potential novel treatments beyond managing CSF flow

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