Cell encapsulation in alginate-based microgels using droplet microfluidics; a review on gelation methods and applications

Mohajeri, Mohammad; Eskandari, Mahnaz; Ghazali, Zahra Sadat; Ghazali, Hanieh Sadat

doi:10.1088/2057-1976/ac4e2d

Cited by 20 publications

(12 citation statements)

References 118 publications

(178 reference statements)

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“…Among all the biomaterials used in this approach, hydrogel-based scaffolds have shown great potential for easy cell encapsulation with biocompatibility and biomimetic of ECM properties, as they can mimic the 3D cell environment of natural tissues. The hydrogel-based scaffold should induce changes in cell functions to regulate cell fate (proliferation, migration, and differentiation) by withstanding biomechanical loads and allowing nutrient transport [67,68]. To design a hydrogel scaffold that delivers biomechanical stimulation to cells, it is important to study its polymer species and their properties and stimulation approaches.…”

Section: D Culture Microenvironment In Cell-encapsulated Hydrogelsmentioning

confidence: 99%

Progress in biomechanical stimuli on the cell-encapsulated hydrogels for cartilage tissue regeneration

Taheri

Ghazali

et al. 2023

Biomater Res

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Background Worldwide, many people suffer from knee injuries and articular cartilage damage every year, which causes pain and reduces productivity, life quality, and daily routines. Medication is currently primarily used to relieve symptoms and not to ameliorate cartilage degeneration. As the natural healing capacity of cartilage damage is limited due to a lack of vascularization, common surgical methods are used to repair cartilage tissue, but they cannot prevent massive damage followed by injury. Main body Functional tissue engineering has recently attracted attention for the repair of cartilage damage using a combination of cells, scaffolds (constructs), biochemical factors, and biomechanical stimuli. As cyclic biomechanical loading is the key factor in maintaining the chondrocyte phenotype, many studies have evaluated the effect of biomechanical stimulation on chondrogenesis. The characteristics of hydrogels, such as their mechanical properties, water content, and cell encapsulation, make them ideal for tissue-engineered scaffolds. Induced cell signaling (biochemical and biomechanical factors) and encapsulation of cells in hydrogels as a construct are discussed for biomechanical stimulation-based tissue regeneration, and several notable studies on the effect of biomechanical stimulation on encapsulated cells within hydrogels are discussed for cartilage regeneration. Conclusion Induction of biochemical and biomechanical signaling on the encapsulated cells in hydrogels are important factors for biomechanical stimulation-based cartilage regeneration.

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Section: D Culture Microenvironment In Cell-encapsulated Hydrogelsmentioning

confidence: 99%

Progress in biomechanical stimuli on the cell-encapsulated hydrogels for cartilage tissue regeneration

Taheri

Ghazali

et al. 2023

Biomater Res

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“…In the literature, natural tissue conjugates such as organ or tissue decellularized matrices or collagen, gelatin, Matrigel, alginate have been suggested as ideal structures for scaffold based 3D culture [68][69][70][71][72][73][74][75][76]. The autologous cell derived extracellular matrix developed within the scope of this study can be also a good resource that would be used as an alternative to these materials or in combination with them.…”

Section: Resultsmentioning

confidence: 99%

The role of decellularized cell derived extracellular matrix in the establishment and culture of in vitro breast cancer tumor model

Tevlek

2024

Biomed. Mater.

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Decades of research have shown that 2D cell culture studies are insufficient for preclinical cancer diagnosis and treatment, and that cancer cells in 3D culture systems have better cell-cell and cell-matrix interactions, gene expression, heterogeneity, and structural complexity that more closely resemble in vivo tumors. Researchers are still optimizing 3D culturing settings for different cancers. Despite promising tumor spheroid research, tumor cell-only aggregates lack the tumor microenvironment and cannot model tumors. Here, MCF-7 breast cancer cell derived decellularized extracellular matrix (CD-dECMs) were obtained and converted into autologous, biologically active, biocompatible, and non-immunogenic hydrogels to be used as micro-environment in both organoid formation and culture. For the production of organoids, CD-dECM doping concentrations ranging from 0.1 mg/mL to 1.5 mg/mL were evaluated, and the lowest concentration was found to be the most effective. For organoid culture, 8 mg/mL CD-dECM, 4 mg/mL rat tendon collagen type I (Col I) (4 mg/mL) and a 1:1 (v/v) mixture of these two were used and the most viable and the biggest organoids were discovered in CD-dECM/Col I (1:1) group. The results show that autologous CD-dECM can replace hydrogels in tumor organoid generation and culture at low and high concentrations, respectively.

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“…Alginate can encapsulate cells, is easily crosslinked, and can be chemically modified for additional crosslinking capabilities. [48,50] It is most often extracted from brown algae and then further processed to obtain alginic acid, but alginate can also be biosynthesized by some species of Azotobacter as well as Pseudomonas. [48] Carrageenans are polysaccharides extracted from red algae of the class Rhodophyceae.…”

Section: Natural Bioinksmentioning

confidence: 99%

3D Bioprinting and Its Role in a Wound Healing Renaissance

Tanfani,

Monpara,

Jonnalagadda

2023

Adv Materials Technologies

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Abstract3D printing (3DP) is an accessible platform being increasingly utilized by independent researchers in fields ranging from industrial manufacturing to medicine. 3D bioprinting is a form of 3D printing that makes use of “bioinks,” organic or synthetic polymer formulations that are often laden with cells. The wide range of materials available means that bioinks can be tailored to the types of tissue that they are meant to emulate. Human skin has a complex microenvironment consisting of numerous layers, cell types, growth factors, and molecular signaling pathways. 3D bioprinting's flexibility and ability to create complex, bioactive structures means that it has great potential in creating artificial skin constructs for skin wound regeneration. While the available materials and cell types for 3DP are large, an ideal bioink for printing artificial skin remains yet to be found. This review will cover the various types of 3DP, the bioinks commonly used, the functionalization of printed artificial skin constructs, challenges that arise in the 3D bioprinting of skin, and future directions for the field. The structure of skin and the wound healing process will also be discussed, as sufficient understanding of these subjects is key to the development of therapeutic artificial skin constructs.

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Cell encapsulation in alginate-based microgels using droplet microfluidics; a review on gelation methods and applications

Cited by 20 publications

References 118 publications

Progress in biomechanical stimuli on the cell-encapsulated hydrogels for cartilage tissue regeneration

Progress in biomechanical stimuli on the cell-encapsulated hydrogels for cartilage tissue regeneration

The role of decellularized cell derived extracellular matrix in the establishment and culture of in vitro breast cancer tumor model

3D Bioprinting and Its Role in a Wound Healing Renaissance

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